Full Text:   <1893>

CLC number: R742.1

On-line Access: 

Received: 2010-08-30

Revision Accepted: 2011-07-27

Crosschecked: 2011-08-09

Cited: 1

Clicked: 4090

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.9 P.704-711

http://doi.org/10.1631/jzus.B1000316


Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus


Author(s):  Zhou-yan Feng, Xiao-jing Zheng, Cong Tian, Yang Wang, Hao-yu Xing

Affiliation(s):  Ministry of Education Key Lab of Biomedical Engineering, College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   hnfzy@yahoo.com.cn

Key Words:  Epilepsy, Paired-pulse depression, γ, -Aminobutyric acid (GABA), Picrotoxin (PTX), CA1 region, In-vivo


Zhou-yan Feng, Xiao-jing Zheng, Cong Tian, Yang Wang, Hao-yu Xing. Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus[J]. Journal of Zhejiang University Science B, 2011, 12(9): 704-711.

@article{title="Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus",
author="Zhou-yan Feng, Xiao-jing Zheng, Cong Tian, Yang Wang, Hao-yu Xing",
journal="Journal of Zhejiang University Science B",
volume="12",
number="9",
pages="704-711",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000316"
}

%0 Journal Article
%T Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus
%A Zhou-yan Feng
%A Xiao-jing Zheng
%A Cong Tian
%A Yang Wang
%A Hao-yu Xing
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 9
%P 704-711
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000316

TY - JOUR
T1 - Changes of paired-pulse evoked responses during the development of epileptic activity in the hippocampus
A1 - Zhou-yan Feng
A1 - Xiao-jing Zheng
A1 - Cong Tian
A1 - Yang Wang
A1 - Hao-yu Xing
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 9
SP - 704
EP - 711
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000316


Abstract: 
Dysfunction of inhibitory synaptic transmission can destroy the balance between excitatory and inhibitory synaptic inputs in neurons, thereby inducing epileptic activity. The aim of the paper is to investigate the effects of successive excitatory inputs on the epileptic activity induced in the absence of inhibitions. Paired-pulse orthodromic and antidromic stimulations were used to test the changes in the evoked responses in the hippocampus. picrotoxin (PTX), γ;-Aminobutyric acid (GABA) type A (GABAA) receptor antagonist, was added to block the inhibitory synaptic transmission and to establish the epileptic model. Extracellular evoked population spike (PS) was recorded in the CA1 region of the hippocampus. The results showed that the application of PTX induced a biphasic change in the paired-pulse ratio of PS amplitude. A short latency increase of the second PS (PS2) was later followed by a reappearance of PS2 depression. This type of depression was observed in both orthodromic and antidromic paired-pulse responses, whereas the GABAergic PS2 depression [called paired-pulse depression (PPD)] during baseline recordings only appeared in orthodromic-evoked responses. In addition, the depression duration at approximately 100 ms was consistent with a relative silent period observed within spontaneous burst discharges induced by prolonged application of PTX. In conclusion, the neurons may ignore the excitatory inputs and intrinsically generate bursts during epileptic activity. The depolarization block could be the mechanisms underlying the PPD in the absence of GABAA inhibitions. The distinct neuronal responses to stimulations during different epileptic stages may implicate the different antiepileptic effects of electrical stimulation.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Albertson, T.E., Walby, W.F., Stark, L.G., Joy, R.M., 1996. The effect of propofol on CA1 pyramidal cell excitability and GABAA-mediated inhibition in the rat hippocampal slice. Life Sci., 58(26):2397-2407.

[2]Alger, B.E., Nicoll, R.A., 1980. Epileptiform burst afterhyperolarization: calcium-dependent potassium potential in hippocampal CA1 pyramidal cells. Science, 210(4474):1122-1124.

[3]Alger, B.E., Nicoll, R.A., 1982. Feed-forward dendritic inhibition in rat hippocampal pyramidal cells studied in vitro. J. Physiol., 328:105-123.

[4]Badawy, R.A., Harvey, A.S., Macdonell, R.A., 2009. Cortical hyperexcitability and epileptogenesis: understanding the mechanisms of epilepsy–Part 1. J. Clin. Neurosci., 16(3):355-365.

[5]Bikson, M., Hahn, P.J., Fox, J.E., Jefferys, J.G., 2003. Depolarization block of neurons during maintenance of electrographic seizures. J. Neurophysiol., 90(4):2402-2408.

[6]Cunha-Reis, D., Sebastiao, A.M., Wirkner, K., Illes, P., Ribeiro, J.A., 2004. VIP enhances both pre- and postsynaptic GABAergic transmission to hippocampal interneurones leading to increased excitatory synaptic transmission to CA1 pyramidal cells. Br. J. Pharmacol., 143(6):733-744.

[7]de Almeida, A.C., Rodrigues, A.M., Scorza, F.A., Cavalheiro, E.A., Teixeira, H.Z., Duarte, M.A., Silveira, G.A., Arruda, E.Z., 2008. Mechanistic hypotheses for nonsynaptic epileptiform activity induction and its transition from the interictal to ictal state–computational simulation. Epilepsia, 49(11):1908-1924.

[8]de Curtis, M., Avanzini, G., 2001. Interictal spikes in focal epileptogenesis. Prog. Neurobiol., 63(5):541-567.

[9]Dzhala, V.I., Staley, K.J., 2003. Transition from interictal to ictal activity in limbic networks in vitro. J. Neurosci., 23(21):7873-7880.

[10]Feng, Z.Y., Zheng, X.J., Wang, J., 2009. Effects of carnosine on the evoked potentials in hippocampal CA1 region. J. Zhejiang Univ. Sci.-B, 10(7):505-511.

[11]Freund, T.F., Buzsaki, G., 1996. Interneurons of the hippocampus. Hippocampus, 6(4):347-470.

[12]Hablitz, J.J., 1984. Picrotoxin-induced epileptiform activity in hippocampus: role of endogenous versus synaptic factors. J. Neurophysiol., 51(5):1011-1027.

[13]Hablitz, J.J., 2004. Regulation of circuits and excitability: implications for epileptogenesis. Epilepsy Curr., 4(4):151-153.

[14]Karnup, S., Stelzer, A., 1999. Temporal overlap of excitatory and inhibitory afferent input in guinea-pig CA1 pyramidal cells. J. Physiol., 516(P2):485-504.

[15]Kloosterman, F., Peloquin, P., Leung, L.S., 2001. Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation. J. Neurophysiol., 86(5):2435-2444.

[16]Leung, L.S., Peloquin, P., Canning, K.J., 2008. Paired-pulse depression of excitatory postsynaptic current sinks in hippocampal CA1 in vivo. Hippocampus, 18(10):1008-1020.

[17]Liu, X., Leung, L.S., 2004. Sodium-activated potassium conductance participates in the depolarizing afterpotential following a single action potential in rat hippocampal CA1 pyramidal cells. Brain Res., 1023(2):185-192.

[18]Margineanu, D.G., Wulfert, E., 2000. Differential paired-pulse effects of gabazine and bicuculline in rat hippocampal CA3 area. Brain Res. Bull., 51(1):69-74.

[19]Muller, W., Misgeld, U., 1991. Picrotoxin- and 4-amino-pyridine-induced activity in hilar neurons in the guinea pig hippocampal slice. J. Neurophysiol., 65(1):141-147.

[20]Papatheodoropoulos, C., Kostopoulos, G., 1998. Development of a transient increase in recurrent inhibition and paired-pulse facilitation in hippocampal CA1 region. Brain Res. Dev., 108(1-2):273-285.

[21]Schiller, Y., Bankirer, Y., 2007. Cellular mechanisms underlying antiepileptic effects of low- and high-frequency electrical stimulation in acute epilepsy in neocortical brain slices in vitro. J. Neurophysiol., 97(3):1887-1902.

[22]Su, Y., Radman, T., Vaynshteyn, J., Parra, L.C., Bikson, M., 2008. Effects of high-frequency stimulation on epileptiform activity in vitro: ON/OFF control paradigm. Epilepsia, 49(9):1586-1593.

[23]Uruno, K., O′Connor, M.J., Masukawa, L.M., 1995. Effects of bicuculline and baclofen on paired-pulse depression in the dentate gyrus of epileptic patients. Brain Res., 695(2):163-172.

[24]Wierenga, C.J., Wadman, W.J., 2003. Functional relation between interneuron input and population activity in the rat hippocampal cornu ammonis 1 area. Neuroscience, 118(4):1129-1139.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE