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CLC number: R734.2

On-line Access: 2011-10-08

Received: 2011-05-20

Revision Accepted: 2011-08-30

Crosschecked: 2011-09-15

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.10 P.828-834

http://doi.org/10.1631/jzus.B1100165


Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma


Author(s):  Kai Wang, Min Zhang, Ying-ying Qian, Zhe-yuan Ding, Jun-hui Lv, Hua-hao Shen

Affiliation(s):  Department of Respiratory Medicine, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China, School of Medicine, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   hh_shen@yahoo.com.cn

Key Words:  Mitogen-activated protein kinase phosphatase-1 (MKP-1), Extracellular signal-regulated kinase (ERK), Lung squamous cell carcinoma (SCC), Prognostic factor


Kai Wang, Min Zhang, Ying-ying Qian, Zhe-yuan Ding, Jun-hui Lv, Hua-hao Shen. Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma[J]. Journal of Zhejiang University Science B, 2011, 12(10): 828-834.

@article{title="Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma",
author="Kai Wang, Min Zhang, Ying-ying Qian, Zhe-yuan Ding, Jun-hui Lv, Hua-hao Shen",
journal="Journal of Zhejiang University Science B",
volume="12",
number="10",
pages="828-834",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100165"
}

%0 Journal Article
%T Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma
%A Kai Wang
%A Min Zhang
%A Ying-ying Qian
%A Zhe-yuan Ding
%A Jun-hui Lv
%A Hua-hao Shen
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 10
%P 828-834
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100165

TY - JOUR
T1 - Imbalanced expression of mitogen-activated protein kinase phosphatase-1 and phosphorylated extracellular signal-regulated kinases in lung squamous cell carcinoma
A1 - Kai Wang
A1 - Min Zhang
A1 - Ying-ying Qian
A1 - Zhe-yuan Ding
A1 - Jun-hui Lv
A1 - Hua-hao Shen
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 10
SP - 828
EP - 834
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100165


Abstract: 
Objective: Mitogen-activated protein kinases (MAPKs) are correlated with a more malignant phenotype in many cancers. This study was designed to evaluate the predictive value of the expression of MAPK phosphatase-1 (MKP-1) and phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), as the key regulatory mechanism of the MAPKs, in lung squamous cell carcinoma (SCC). Methods: We assessed the expressions of MKP-1 and p-ERK1/2 in twenty subjects at different differentiation degree of SCC and five normal lungs by immunohistochemistry and real-time reverse transcriptase polymerase chain reaction (RT-PCR) analysis. Results: Immunohistochemistry and real-time RT-PCR assay showed that the expression of MKP-1 was gradually decreased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was negatively correlated with tumor differentiation (P<0.01). However, the expression of p-ERK1/2 or ERK1/2 was gradually increased as tissue type went from normal lung tissues to increasingly undifferentiated carcinoma, and it was positively correlated with tumor differentiation (P<0.01). Conclusions: Our data indicates the relevance of MKP-1 and p-ERK1/2 in SCC as a potential positive and negative prognostic factor. The imbalanced expression of MKP-1 and p-ERK1/2 may play a role in the development of SCC and these two molecules may be new targets for the therapy and prognosis of SCC.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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