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CLC number: R96

On-line Access: 2011-10-08

Received: 2011-03-04

Revision Accepted: 2011-05-19

Crosschecked: 2011-09-14

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.10 P.835-845

http://doi.org/10.1631/jzus.B1100067


Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells


Author(s):  Yu-hua Ran, Hai Wang

Affiliation(s):  Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Beijing 100850, China, Thadweik Academy of Medicine, Beijing 100039, China

Corresponding email(s):   wh9558@yahoo.cn

Key Words:  Neurovascular unit, Cerebral ischemia/reperfusion (I/R) injury, ATP-sensitive potassium channel opener, Neuroprotection, Apoptosis


Yu-hua Ran, Hai Wang. Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells[J]. Journal of Zhejiang University Science B, 2011, 12(10): 835-845.

@article{title="Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells",
author="Yu-hua Ran, Hai Wang",
journal="Journal of Zhejiang University Science B",
volume="12",
number="10",
pages="835-845",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100067"
}

%0 Journal Article
%T Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
%A Yu-hua Ran
%A Hai Wang
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 10
%P 835-845
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100067

TY - JOUR
T1 - Iptakalim, an ATP-sensitive potassium channel opener, confers neuroprotection against cerebral ischemia/reperfusion injury in rats by protecting neurovascular unit cells
A1 - Yu-hua Ran
A1 - Hai Wang
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 10
SP - 835
EP - 845
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100067


Abstract: 
Objective: To investigate the role of iptakalim, an ATP-sensitive potassium channel opener, in transient cerebral ischemia/reperfusion (I/R) injury and its involved mechanisms. Methods: Intraluminal occlusion of middle cerebral artery (MCAO) in a rat model was used to investigate the effect of iptakalim at different time points. Infarct volume was measured by staining with 2,3,5-triphenyltetrazolium chloride, and immunohistochemistry was used to evaluate the expressions of Bcl-2 and Bax. In vitro, neurovascular unit (NVU) cells, including rat primary cortical neurons, astrocytes, and cerebral microvascular endothelial cells, were cultured and underwent oxygen-glucose deprivation (OGD). The protective effect of iptakalim on NVU cells was investigated by cell viability and injury assessments, which were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and release of lactate dehydrogenase. Caspase-3, Bcl-2 and Bax mRNA expressions were evaluated by real-time polymerase chain reaction (PCR). Results: Administration of iptakalim 0 or 1 h after reperfusion significantly reduced infarct volumes, improved neurological scores, and attenuated brain edema after cerebral I/R injury. Iptakalim treatment (0 h after reperfusion) also reduced caspase-3 expression and increased the ratio of Bcl-2 to Bax by immunohistochemistry. Iptakalim inhibited OGD-induced cell death in cultured neurons and astrocytes, and lactate dehydrogenase release from cerebral microvascular endothelial cells. Iptakalim reduced mRNA expression of caspase-3 and increased the ratio of Bcl-2 to Bax in NVU cells. Conclusions: Iptakalim confers neuroprotection against cerebral I/R injury by protecting NVU cells via inhibiting of apoptosis.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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Open peer comments: Debate/Discuss/Question/Opinion

<1>

Cuiping Liu@nanfang hosptial<bullebell@163.com>

2011-10-11 10:16:19

this is a very interesting paperm and I want to read the fulltext

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