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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.9 P.774-784

http://doi.org/10.1631/jzus.B1200277


Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting


Author(s):  Nadeem Akhtar Abbasi, Tariq Pervaiz, Ishfaq Ahmed Hafiz, Mehwish Yaseen, Azhar Hussain

Affiliation(s):  Plant Tissue Culture Laboratory, Department of Horticulture, Pir Mehr Ali Shah-Arid Agriculture University, Rawalpindi 46300, Pakistan

Corresponding email(s):   nadeemabbasi65@yahoo.com

Key Words:  Eriobotrya japonica, Micropropagation, Sterilization of loquat, Plant growth regulator, Shoot proliferation, Rhizogenesis


Nadeem Akhtar Abbasi, Tariq Pervaiz, Ishfaq Ahmed Hafiz, Mehwish Yaseen, Azhar Hussain. Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting[J]. Journal of Zhejiang University Science B, 2013, 14(9): 774-784.

@article{title="Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting",
author="Nadeem Akhtar Abbasi, Tariq Pervaiz, Ishfaq Ahmed Hafiz, Mehwish Yaseen, Azhar Hussain",
journal="Journal of Zhejiang University Science B",
volume="14",
number="9",
pages="774-784",
year="2013",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1200277"
}

%0 Journal Article
%T Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting
%A Nadeem Akhtar Abbasi
%A Tariq Pervaiz
%A Ishfaq Ahmed Hafiz
%A Mehwish Yaseen
%A Azhar Hussain
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 9
%P 774-784
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200277

TY - JOUR
T1 - Assessing the response of indigenous loquat cultivar Mardan to phytohormones for in vitro shoot proliferation and rooting
A1 - Nadeem Akhtar Abbasi
A1 - Tariq Pervaiz
A1 - Ishfaq Ahmed Hafiz
A1 - Mehwish Yaseen
A1 - Azhar Hussain
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 9
SP - 774
EP - 784
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200277


Abstract: 
In vitro cultures of loquat cultivar Mardan were established using shoot apices after treating with NaOCl (5%, 7%, 10%, 12%, 14% (v/v)) for 12 min and HgCl2 (0.01%, 0.05%, 0.10%, 0.20%, 0.25% (w/v)) for 2 min. A maximum survival rate of 70% was recorded after surface sterilization with 10% NaOCl. Caulogenic response was assessed on Murashige and Skoog (MS) medium fortified with assorted combinations of the cytokinins, benzylaminopurine (BAP), kinetin, and N6-(2-isopentyl)adenine (2iP). Treatment of BAP 1.5 mg/L combined with 2iP 9.0 mg/L and kinetin 1.5 mg/L was found to be optimum for shoot morphogenesis in terms of the number and subsequent growth of shoots, while the highest shoot length was yielded by the combination of BAP 0.5 mg/L, kinetin 0.5 mg/L, and 2iP 3 mg/L. Higher levels of cytokinins induced callogenesis, vitrification and stunted growth to some extent. For rhizogenesis, uniform sized micro-shoots were excised and transferred to half-strength MS medium containing auxins. The best rooting expression was observed with naphthaleneacetic acid (NAA) 1 mg/L combined with indole-3-butyric acid (IBA) 2 mg/L and paclobutrazol (PBZ) 1 mg/L.

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