Full Text:   <2471>

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CLC number: S855

On-line Access: 2017-06-05

Received: 2016-03-24

Revision Accepted: 2016-06-27

Crosschecked: 2017-05-10

Cited: 0

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Citations:  Bibtex RefMan EndNote GB/T7714


Da-wei Yao


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Journal of Zhejiang University SCIENCE B 2017 Vol.18 No.6 P.474-480


Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium

Author(s):  Da-wei Yao, Ze-zhong Yu, Na Li, Yu-nong Hou, Jia-rong Xu, De-ji Yang

Affiliation(s):  College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China; more

Corresponding email(s):   djyang@njau.edu.cn

Key Words:  Copper, Palygorskite, Diarrhea, Salmonella

Da-wei Yao, Ze-zhong Yu, Na Li, Yu-nong Hou, Jia-rong Xu, De-ji Yang. Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium[J]. Journal of Zhejiang University Science B, 2017, 18(6): 474-480.

@article{title="Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium",
author="Da-wei Yao, Ze-zhong Yu, Na Li, Yu-nong Hou, Jia-rong Xu, De-ji Yang",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium
%A Da-wei Yao
%A Ze-zhong Yu
%A Na Li
%A Yu-nong Hou
%A Jia-rong Xu
%A De-ji Yang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 6
%P 474-480
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600133

T1 - Copper-modified palygorskite is effective in preventing and treating diarrhea caused by Salmonella typhimurium
A1 - Da-wei Yao
A1 - Ze-zhong Yu
A1 - Na Li
A1 - Yu-nong Hou
A1 - Jia-rong Xu
A1 - De-ji Yang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 6
SP - 474
EP - 480
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600133

The aim of this research was to develop effective alternative therapies to reduce antibiotic use in animal agriculture. In this study, the efficacy of copper-modified palygorskite (CM-Pal) in preventing diarrhea caused by Salmonella was specifically examined both in vitro and in vivo. The CM-Pal was prepared with palygorskite (Pal) and copper nitrate. The antibacterial activity of the CM-Pal was detected by comparing the differences in cell numbers on plate count agar before and after adding the CM-Pal to Salmonella typhimurium cultures. Seventy ICR mice were then allocated into seven groups. Five groups (the treatment groups) were infected with S. typhimurium by intraperitoneal (i.p.) injection and were given Pal, CM-Pal, montmorillonite powder, gentamicin, and physiological saline, respectively. One group (the prevention group) was given CM-Pal before infection with S. typhimurium. Another group (the uninfected group) was not infected with S. typhimurium. The effects of Pal, CM-Pal, montmorillonite powder, and gentamicin on the treatment or prevention of diarrhea in the mice were examined by stool studies, fecal scoring, and assessment of growth performance and villus height. The CM-Pal had satisfactory anti-bacterial properties in vitro: the antibacterial rate was 100% after 2 h incubation with S. typhimurium NJS1 cultures (1×106 colony-forming units (CFU)/ml). In the in vivo experiment, the CM-Pal exerted superior effects in the treatment and prevention of diarrhea in mice compared with Pal, montmorillonite powder, and gentamicin. In the CM-Pal group, no mice showed signs of diarrhea at 24 h post infection (p.i.), and all mice fully recovered from infection. However, the Pal group, montmorillonite group, and gentamicin group only recovered after 48, 48, and 96 h, respectively. The villus height level in the CM-Pal treatment group recovered at 3 d p.i. However, the recovery time of the other groups was longer (at least 5 d). The CM-Pal prevention group had a better effect on weight gain than the other groups. This study suggested that CM-Pal may be an effective alternative to conventional antibiotics for the treatment and prevention of animal diarrhea caused by Salmonella.


结论:体外抑菌试验显示,CM-Pal与沙门氏菌作用2 h后抗菌率达100%。体内试验也显示,CM-Pal组小鼠24 h后腹泻症状消失,所有小鼠恢复正常。但是其他组小鼠(Pal组、蒙脱石散组和庆大霉素组)分别在48、48和96 h后才恢复正常。CM-Pal治疗组小鼠肠绒毛的高度在感染3 d后恢复正常,但是其他组小鼠肠绒毛高度恢复正常至少需要 5 d。CM-Pal预防组小鼠增重高于其他组小鼠。本研究表明,CM-Pal在预防和治疗由沙门氏菌引起的腹泻方面能够替代抗生素具有较好的效果。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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