CLC number: R735.2
On-line Access: 2017-01-03
Received: 2016-05-10
Revision Accepted: 2016-08-01
Crosschecked: 2016-12-13
Cited: 1
Clicked: 4736
Zhong-dong Wang, Fan-yong Qu, Yuan-yuan Chen, Zhang-shen Ran, Hai-yan Liu, Hai-dong Zhang. Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells[J]. Journal of Zhejiang University Science B, 2017, 18(1): 27-36.
@article{title="Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells",
author="Zhong-dong Wang, Fan-yong Qu, Yuan-yuan Chen, Zhang-shen Ran, Hai-yan Liu, Hai-dong Zhang",
journal="Journal of Zhejiang University Science B",
volume="18",
number="1",
pages="27-36",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600205"
}
%0 Journal Article
%T Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells
%A Zhong-dong Wang
%A Fan-yong Qu
%A Yuan-yuan Chen
%A Zhang-shen Ran
%A Hai-yan Liu
%A Hai-dong Zhang
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 1
%P 27-36
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600205
TY - JOUR
T1 - Involvement of microRNA-718, a new regulator of EGR3, in regulation of malignant phenotype of HCC cells
A1 - Zhong-dong Wang
A1 - Fan-yong Qu
A1 - Yuan-yuan Chen
A1 - Zhang-shen Ran
A1 - Hai-yan Liu
A1 - Hai-dong Zhang
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 1
SP - 27
EP - 36
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600205
Abstract: Objective: hepatocellular carcinoma (HCC) is still one of the most common death-related malignancies worldwide. Because the way onset and progression are hidden most, HCC diagnoses are made at an advanced stage, when they are unsuitable for surgical resection. microRNAs are a class of small non-coding RNAs, participating in many aspects of cancers. In this study, we tried to establish the role of microRNA-718 (miR-718) in the malignant phenotype of HCC cells and its possible role in HCC diagnosis. Methods: Here we first used a methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay, Transwell migration and invasion assays, and colony formation assay to evaluate the impact of miR-718 on the malignant phenotypes of HCC cells. Then, we used bioinformatic methods to predict the target gene of miR-718 and used green fluorescence protein (GFP) reporter assay, Western blot, and quantitative real-time polymerase chain reaction (qRT-PCR) to validate the regulation relationship. Finally, we determined the role of the target gene in the HCC phenotype. Results: We found that the expression of miR-718 was significantly reduced in various HCC cell lines and HCC tissues. Re-expression of miR-718 significantly reduced the cellular viability and colony formation ability as well as inhibited the migration and invasion abilities of HCC cell lines. early growth response protein 3 (EGR3) is a direct target of miR-718 and is negatively regulated by miR-718. EGR3 could increase the viability and proliferation of HCC cells, and promot the migration and invasion of HCC cells. Conclusions: miR-718 acts as a tumor suppressive microRNA in HCC via regulating the expression of EGR3, which may provide a new diagnostic marker and treatment target for HCC.
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