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CLC number: TS225.1; TS201.4

On-line Access: 2020-08-04

Received: 2020-03-08

Revision Accepted: 2020-05-11

Crosschecked: 2020-07-10

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Citations:  Bibtex RefMan EndNote GB/T7714


Li-rong Shen


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Journal of Zhejiang University SCIENCE B 2020 Vol.21 No.8 P.657-667


New observations on the effect of camellia oil on fatty liver disease in rats

Author(s):  Chun-xue Li, Li-rong Shen

Affiliation(s):  Department of Food Science and Nutrition, College of Biosystems Engineering and Food Science, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   shenlirong@zju.edu.cn

Key Words:  Camellia oil, Fatty acid, Lipid droplet, Hepatocyte ultrastructure, Organelle

Chun-xue Li, Li-rong Shen. New observations on the effect of camellia oil on fatty liver disease in rats[J]. Journal of Zhejiang University Science B, 2020, 21(8): 657-667.

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author="Chun-xue Li, Li-rong Shen",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T New observations on the effect of camellia oil on fatty liver disease in rats
%A Chun-xue Li
%A Li-rong Shen
%J Journal of Zhejiang University SCIENCE B
%V 21
%N 8
%P 657-667
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%D 2020
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2000101

T1 - New observations on the effect of camellia oil on fatty liver disease in rats
A1 - Chun-xue Li
A1 - Li-rong Shen
J0 - Journal of Zhejiang University Science B
VL - 21
IS - 8
SP - 657
EP - 667
%@ 1673-1581
Y1 - 2020
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B2000101

camellia oil has become an important plant oil in China in recent years, but its effects on non-alcoholic fatty liver disease (NAFLD) have not been documented. In this study, the effects of camellia oil, soybean oil, and olive oil on NAFLD were evaluated by analyzing the fatty acid profiles of the plant oils, the serum lipids and lipoproteins of rats fed different oils, and by cytological and ultrastructural observation of the rats’ hepatocytes. Analysis of fatty acid profiles showed that the polyunsaturated fatty acid (PUFA) n-6/n-3 ratio was 33.33 in camellia oil, 12.50 in olive oil, and 7.69 in soybean oil. Analyses of serum lipids and lipoproteins of rats showed that the levels of total cholesterol and low-density lipoprotein cholesterol in a camellia oil-fed group (COFG) were lower than those in an olive oil-fed group (OOFG) and higher than those in a soybean oil-fed group (SOFG). However, only the difference in total cholesterol between the COFG and SOFG was statistically significant. Cytological observation showed that the degree of lipid droplet (LD) accumulation in the hepatocytes in the COFG was lower than that in the OOFG, but higher than that in the SOFG. Ultrastructural analysis revealed that the size and number of the LDs in the hepatocytes of rats fed each of the three types of oil were related to the degree of damage to organelles, including the positions of nuclei and the integrity of mitochondria and endoplasmic reticulum. The results revealed that the effect of camellia oil on NAFLD in rats was greater than that of soybean oil, but less than that of olive oil. Although the overall trend was that among the three oil diets, those with a lower n-6/n-3 ratio were associated with a lower risk of NAFLD, and the effect of camellia oil on NAFLD was not entirely related to the n-6/n-3 ratio and may have involved other factors. This provides new insights into the effect of oil diets on NAFLD.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1]Araya J, Rodrigo R, Videla LA, et al., 2004. Increase in long-chain polyunsaturated fatty acid n-6/n-3 ratio in relation to hepatic steatosis in patients with non-alcoholic fatty liver disease. Clin Sci, 106(6):635-643.

[2]Boudour-Benrachou N, Plard J, Pinatel C, et al., 2017. Fatty acid compositions of olive oils from six cultivars from East and South-Western Algeria. Adv Food Technol Nutr Sci Open J, 3:1-5.


[4]Caldwell SH, Swerdlow RH, Khan EM, et al., 1999. Mitochondrial abnormalities in non-alcoholic steatohepatitis. J Hepatol, 31(3):430-434.

[5]Cheng YT, Lu CC, Yen GC, 2015. Beneficial effects of camellia oil (Camellia oleifera Abel.) on hepatoprotective and gastroprotective activities. J Nutr Sci Vitaminol, 61(S1):S100-S102.

[6]Clemente TE, Cahoon EB, 2009. Soybean oil: genetic approaches for modification of functionality and total content. Plant Physiol, 151(3):1030-1040.

[7]Ellatif MA, El-Karib AO, Dallak M, et al., 2018. Vitamin E protects against hepatocyte ultrastructural damage induced by high fat diet in a rat model of pre-diabetes. Int J Morphol, 36(4):1350-1355.

[8]Ferramosca A, Zara V, 2014. Modulation of hepatic steatosis by dietary fatty acids. World J Gastroenterol, 20(7):1746-1755.

[9]Fujimoto T, Parton RG, 2011. Not just fat: the structure and function of the lipid droplet. Cold Spring Harb Perspect Biol, 3(3):a004838.

[10]Gluchowski NL, Becuwe M, Walther TC, et al., 2017. Lipid droplets and liver disease: from basic biology to clinical implications. Nat Rev Gastroenterol Hepatol, 14(6):343-355.

[11]Han KH, 2012. Omega-3-fatty acid and triglyceride. Korean J Med, 83(6):724-727.

[12]Ibdah JA, Perlegas P, Zhao YW, et al., 2005. Mice heterozygous for a defect in mitochondrial trifunctional protein develop hepatic steatosis and insulin resistance. Gastroenterology, 128(5):1381-1390.

[13]Juárez-Hernández E, Chávez-Tapia NC, Uribe M, et al., 2016. Role of bioactive fatty acids in nonalcoholic fatty liver disease. Nutr J, 15:72.

[14]Liu XH, Jia LY, Gao Y, et al., 2014. Anti-inflammatory activity of total flavonoids from seeds of Camellia oleifera Abel. Acta Biochim Biophys Sin, 46(10):920-922.

[15]Messina MJ, 1997. Soy foods: their role in disease prevention and treatment. In: Liu SK (Ed.), Soybeans. Springer, Boston, MA, USA, p.698-699.

[16]Monteiro J, Leslie M, Moghadasian MH, et al., 2014. The role of n-6 and n-3 polyunsaturated fatty acids in the manifestation of the metabolic syndrome in cardiovascular disease and non-alcoholic fatty liver disease. Food Funct, 5(3):426-435.

[17]NHFPC (National Health and Family Planning Commission of the People’s Republic of China), CFDA (China Food and Drug Administration), 2016. Determination of fatty acids in food, GB 5009.168-2016. Food Safety National Standard of People’s Republic of China.

[18]Oğraş ŞŞ, Kaban G, Kaya M, 2016. The effects of geographic region, cultivar and harvest year on fatty acid composition of olive oil. J Oleo Sci, 65(11):889-895.

[19]Pessayre D, Fromenty B, 2005. NASH: a mitochondrial disease. J Hepatol, 42(6):928-940.

[20]Prabakaran M, Lee KJ, An Y, et al., 2018. Changes in soybean (Glycine max L.) flour fatty-acid content based on storage temperature and duration. Molecules, 23(10):2713.

[21]Sanyal AJ, Campbell-Sargent C, Mirshahi F, et al., 2001. Nonalcoholic steatohepatitis: association of insulin resistance and mitochondrial abnormalities. Gastroenterology, 120(5):1183-1192.

[22]Wang L, Wang Y, Liang Y, et al., 2013. Specific accumulation of lipid droplets in hepatocyte nuclei of PFOA-exposed BALB/c mice. Sci Rep, 3:2174.

[23]Wang XQ, Zeng QM, Verardo V, et al., 2017a. Fatty acid and sterol composition of tea seed oils: their comparison by the “FancyTiles” approach. Food Chem, 233:302-310.

[24]Wang XQ, Zeng QM, Del Mar Contreras M, et al., 2017b. Profiling and quantification of phenolic compounds in Camellia seed oils: natural tea polyphenols in vegetable oil. Food Res Int, 102:184-194.

[25]Wei YZ, Rector RS, Thyfault JP, et al., 2008. Nonalcoholic fatty liver disease and mitochondrial dysfunction. World J Gastroenterol, 14(2):193-199.

[26]Wilfling F, Wang HJ, Haas JT, et al., 2013. Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Dev Cell, 24(4):384-399.

[27]Xiao XM, He LM, Chen YY, et al., 2017. Anti-inflammatory and antioxidative effects of Camellia oleifera Abel. components. Future Med Chem, 9(17):2069-2079.

[28]Yang CY, Liu XM, Chen ZY, et al., 2016. Comparison of oil content and fatty acid profile of ten new Camellia oleifera cultivars. J Lipids, 2016:3982486.

[29]Zeng W, Endo Y, 2019. Lipid characteristics of camellia seed oil. J Oleo Sci, 68(7):649-658.

[30]Zhang JZ, Chu FQ, Guo DP, et al., 2012. Cytology and ultrastructure of interactions between Ustilago esculenta and Zizania latifolia. Mycol Prog, 11(2):499-508.

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