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On-line Access: 2023-01-10
Received: 2022-08-30
Revision Accepted: 2022-10-26
Crosschecked: 2023-01-16
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Citations: Bibtex RefMan EndNote GB/T7714
Xun WANG, Jinqiu HUANG, Zongyan YIN, Ke XU, Dong JIANG, Lijin LIN, Xiaoai ZHANG, Zhihui WANG. Carotenoid components and their biosynthesis in a bud mutant of Shiranui mandarin (Citrus reticulata Blanco) with citrine flavedo[J]. Journal of Zhejiang University Science B,in press.Frontiers of Information Technology & Electronic Engineering,in press.https://doi.org/10.1631/jzus.B2200431 @article{title="Carotenoid components and their biosynthesis in a bud mutant of Shiranui mandarin (Citrus reticulata Blanco) with citrine flavedo", %0 Journal Article TY - JOUR
"不知火"黄色果皮芽变品种中类胡罗卜素组分及生物合成1四川农业大学果蔬研究所,中国成都市,611130 2四川农业大学园艺学院,中国成都市,611130 3四川省园艺作物技术推广总站,中国成都市,610041 4西南大学柑桔研究所,中国重庆市,400712 摘要:宽皮柑橘"柠檬不知火"是"不知火"的自然芽变品种,其果皮呈类似柠檬果皮的亮黄色,明显区别于"不知火"果皮的橘红色。本研究通过分析上述两种柑橘果皮中类胡罗卜素的含量和组分,及与其合成代谢相关基因的表达量,解析两种柑橘果皮颜色的差异机制。测定结果显示,"不知火"中总类胡罗卜素含量显著高于芽变品种"柠檬不知火",推测这是造成两种果皮颜色差异的主要原因。芽变品种中呈现橙色色素的组分β-隐黄质占比低于"不知火";而与绿-黄色调有关的叶黄素和新黄质这两种类胡罗卜素在芽变品种中的占比高于"不知火"。"不知火"中PSY基因的高表达可能为其类胡罗卜素合成提供了充足的底物,从而促使其果皮中较高水平的类胡罗卜素合成;HYb基因在所有基因中表达量最高,推测与其参与催化类胡罗卜素代谢途径中多个反应有关;而ZEP基因的低表达量可能与类胡罗卜素代谢途径下游产物的反馈调节有关。 关键词组: Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article
Reference[1]AgócsA, NagyV, SzabóZ, et al., 2007. Comparative study on the carotenoid composition of the peel and the pulp of different citrus species. Innov Food Sci Emerg Technol, 8(3):390-394. [2]AlquezarB, RodrigoMJ, ZacaríasL, 2008. Regulation of carotenoid biosynthesis during fruit maturation in the red-fleshed orange mutant Cara Cara. Phytochemistry, 69(10):1997-2007. [3]AlquezarB, RodrigoMJ, LadoJ, et al., 2013. A comparative physiological and transcriptional study of carotenoid biosynthesis in white and red grapefruit (Citrus paradisi Macf.). Tree Genet Genomes, 9(5):1257-1269. [4]ChenCX, 2020. Pigments in citrus fruit: mutants, compounds, genes, and beyond. In: Gentile A, la Malfa S, Deng ZN (Eds.), The Citrus Genome. Springer, Cham, p.195-209. [5]FengGZ, WuJX, XuYH, et al., 2021. High-spatiotemporal-resolution transcriptomes provide insights into fruit development and ripening in Citrus sinensis. Plant Biotechnol J, 19(7):1337-1353. [6]HirschbergJ, 2001. Carotenoid biosynthesis in flowering plants. Curr Opin Plant Biol, 4(3):210-218. [7]KatoM, IkomaY, MatsumotoH, et al., 2004. Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit. Plant Physiol, 134(2):824-837. [8]LiMY, LiJ, TanHH, et al., 2022. Comparative metabolomics provides novel insights into the basis of petiole color differences in celery (Apium graveolens L.). J Zhejiang Univ-Sci B (Biomed & Biotechnol), 23(4):300-314. [9]RodrigoMJ, MarcosJF, AlférezF, et al., 2003. Characterization of Pinalate, a novel Citrus sinensis mutant with a fruit-specific alteration that results in yellow pigmentation and decreased ABA content. J Exp Bot, 54(383):727-738. [10]Rodríguez-VillalónA, GasE, Rodríguez-ConcepciónM, 2009. Phytoene synthase activity controls the biosynthesis of carotenoids and the supply of their metabolic precursors in dark-grown Arabidopsis seedlings. Plant J, 60(3):424-435. [11]Ruiz-SolaMÁ, Rodríguez-ConcepciónM, 2012. Carotenoid biosynthesis in Arabidopsis: a colorful pathway. Arabidopsis Book, 2012(10):e0158. [12]SainiRK, NileSH, ParkSW, 2015. Carotenoids from fruits and vegetables: chemistry, analysis, occurrence, bioavailability and biological activities. Food Res Int, 76(Part 3):735-750. [13]SongXM, LiN, ZhangYC, et al., 2022. Transcriptomics and genomics analysis uncover the differentially expressed chlorophyll and carotenoid-related genes in celery. Int J Mol Sci, 23(16):8986. [14]StanleyL, YuanYW, 2019. Transcriptional regulation of carotenoid biosynthesis in plants: so many regulators, so little consensus. Front Plant Sci, 10:1017. [15]SumiasihIH, PoerwantoR, EfendiD, et al., 2017. β- [16]Cryptoxanthin and zeaxanthin pigments accumulation to induce orange color on citrus fruits. IOP Conf Ser Mater Sci Eng, 299:012074. [17]YuanPL, UmerMJ, HeN, et al., 2021. Transcriptome regulation of carotenoids in five flesh-colored watermelons (Citrullus lanatus). BMC Plant Biol, 21:203. Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou
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