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Bio-Design and Manufacturing  2023 Vol.6 No.4 P.423-438

http://doi.org/10.1007/s42242-023-00237-3


Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering


Author(s):  Yuriy Snyder & Soumen Jana

Affiliation(s):  Department of Bioengineering, University of Missouri, Columbia, MO 65211, USA

Corresponding email(s):   sjgv7@missouri.edu

Key Words:  Heart valve leaflet, Trilayer, Tissue engineering, Fiber, Electrospinning


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Yuriy Snyder & Soumen Jana. Trilayer anisotropic structure versus randomly oriented structure in heart valve leaflet tissue engineering[J]. Journal of Zhejiang University Science D, 2023, 6(4): 423-438.

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Abstract: 
It has been hypothesized that leaflet substrates with a trilayer structure and anisotropic mechanical properties could be useful for the production of functional and long-lasting tissue-engineered leaflets. To investigate the influence of the anisotropic structural and mechanical characteristics of a substrate on cells, in this study, we electrospun trilayer anisotropic fibrous substrates and randomly oriented isotropic fibrous substrates (used as controls) from polycaprolactone polymers. Consequently, the random substrates had higher radial and lower circumferential tensile properties than the trilayer substrates; however, they had similar flexural properties. Porcine valvular interstitial cells cultured on both substrates produced random and trilayer cell-cultured constructs, respectively. The trilayer cell-cultured constructs had more anisotropic mechanical properties, 17% higher cellular proliferation, 14% more extracellular matrix (i.e., collagen and glycosaminoglycan) production, and superior gene and protein expression, suggesting that more cells were in a growth state in the trilayer constructs than in the random constructs. Furthermore, the random and radial layers of the trilayer constructs had more vimentin, collagen, transforming growth factor-beta 1 (TGF-1), transforming growth factor-beta 3 (TGF-3) gene expression than in the circumferential layer of the constructs. This study verifies that the differences in structural, tensile, and anisotropic properties of the trilayer and random substrates influence the characteristics of the cells and ECM in the constructs.

密苏里大学Soumen Jana等 | 三层各向异性结构与随机取向结构在心脏瓣膜组织工程中的比较

本研究论文聚焦具有定向特征的异质结构在以组织工程法制造心脏瓣膜组的应用。现有假设认为,具有三层结构和各向异性力学特性的瓣膜基质可能对功能性和持久性组织工程瓣膜的制造产生积极作用。为了研究基质的各向异性结构和力学特性对细胞的影响,本研究使用聚己内酯聚合物电纺制备了三层各向异性纤维基质和随机定向各向同性纤维基质(作为对照)。结果表明,随机基质的径向和周向拉伸性能高于具备各向异性的三层基质,但二者的弯曲性能相似。在这两种基质上培养的猪瓣膜间质细胞分别产生了随机和三层细胞培养构建物。三层细胞培养结构具有强的各向异性的力学特性,细胞增殖率高出17%,胞外基质(如胶原蛋白和粘多糖)产量增加14%,并且基因和蛋白表达更优,表明相较于各向同性结构,三层构建物中的细胞处于更好的生长状态。此外,三层构建物的随机和径向层比环向层具有更多的波形蛋白、胶原蛋白、转化生长因子-beta 1(TGF-ß1)和转化生长因子-beta 3(TGF-ß3)基因表达。该研究证实,三层和随机基质的结构、拉伸和各向异性特性可以差异影响所打印结构中细胞和胞外基质的特性。

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