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On-line Access: 2020-02-01

Received: 2020-02-07

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Bio-Design and Manufacturing  2020 Vol.3 No.1 P.30-39

http://doi.org/10.1007/s42242-020-00062-y


3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration


Author(s):  Lei Shao, Qing Gao, Chaoqi Xie, Jianzhong Fu, Meixiang Xiang, Zhenjie Liu, Liulin Xiang, Yong He

Affiliation(s):  State Key Laboratory of Fluid Power and Mechatronic Systems, College of Mechanical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   gaoqingvc@zju.edu.cn, lawson4001@zju.edu.cn, yongqin@zju.edu.cn

Key Words:  Sacrifcial microgel, Gelatin methacryloyl (GelMA), 3D bioprinting, Mesoscale pore networks (MPNs), Tissue engineering


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Lei Shao, Qing Gao, Chaoqi Xie, Jianzhong Fu, Meixiang Xiang, Zhenjie Liu, Liulin Xiang, Yong He. 3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration[J]. Journal of Zhejiang University Science D, 2020, 3(1): 30-39.

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author="Lei Shao, Qing Gao, Chaoqi Xie, Jianzhong Fu, Meixiang Xiang, Zhenjie Liu, Liulin Xiang, Yong He",
journal="Journal of Zhejiang University Science D",
volume="3",
number="1",
pages="30-39",
year="2020",
publisher="Zhejiang University Press & Springer",
doi="10.1007/s42242-020-00062-y"
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%T 3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration
%A Lei Shao
%A Qing Gao
%A Chaoqi Xie
%A Jianzhong Fu
%A Meixiang Xiang
%A Zhenjie Liu
%A Liulin Xiang
%A Yong He
%J Journal of Zhejiang University SCIENCE D
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%I Zhejiang University Press & Springer
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T1 - 3D printing of hydroxyapatite/tricalcium phosphate scafold with hierarchical porous structure for bone regeneration
A1 - Lei Shao
A1 - Qing Gao
A1 - Chaoqi Xie
A1 - Jianzhong Fu
A1 - Meixiang Xiang
A1 - Zhenjie Liu
A1 - Liulin Xiang
A1 - Yong He
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PB - Zhejiang University Press & Springer
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DOI - 10.1007/s42242-020-00062-y


Abstract: 
Three-dimensional (3D) bioprinting is a powerful approach that enables the fabrication of 3D tissue constructs that retain complex biological functions. However, the dense hydrogel networks that form after the gelation of bioinks often restrict the migration and proliferation of encapsulated cells. Herein, a sacrifcial microgel-laden bioink strategy was designed for directly bioprinting constructs with mesoscale pore networks (MPNs) for enhancing nutrient delivery and cell growth. The sacrifcial microgel-laden bioink, which contains cell/gelatin methacryloyl (GelMA) mixture and gelled gelatin microgel, is frst thermo-crosslinked to fabricate temporary predesigned cell-laden constructs by extrusion bioprinting onto a cold platform. Then, the construct is permanently stabilized through photo-crosslinking of GelMA. The MPNs inside the printed constructs are formed after subsequent dissolution of the gelatin microgel. These MPNs allowed for efective oxygen/nutrient difusion, facilitating the generation of bioactive tissues. Specifcally, osteoblast and human umbilical vein endothelial cells encapsulated in the bioprinted large-scale constructs (≥1 cm) with MPNs showed enhanced bioactivity during culture. The 3D bioprinting strategy based on the sacrifcial microgel-laden bioink provided a facile method to facilitate formation of complex tissue constructs with MPNs and set a foundation for future optimization of MPN-based tissue constructs with applications in diverse areas of tissue engineering.

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