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Abstract: Resin-dentin bond degradation is a major cause of restoration failures. The major aim of the current study was to evaluate the impact of a remineralization medium on collagen matrices of hybrid layers of three different adhesive resins using nanotechnology methods. Coronal dentin surfaces were prepared from freshly extracted premolars and bonded to composite resin using three adhesive resins (FluoroBond II, Xeno-III-Bond, and iBond). From each tooth, two central slabs were selected for the study. The slabs used as controls were immersed in a simulated body fluid (SBF). The experimental slabs were immersed in a Portland cement-based remineralization medium that contained two biomimetic analogs (biomineralization medium (BRM)). Eight slabs per group were retrieved after 1, 2, 3, and 4 months, respectively and immersed in Rhodamine B for 24 h. Confocal laser scanning microscopy was used to evaluate the permeability of hybrid layers to Rhodamine B. Data were analyzed by analysis of variance (ANOVA) and Tukey’s honest significant difference (HSD) tests. After four months, all BRM specimens exhibited a significantly smaller fluorescent area than SBF specimens, indicating a remineralization of the hybrid layer (P≤0.05). A clinically applicable biomimetic remineralization delivery system could potentially slow down bond degradation.

牙本质粘结混合层仿生再矿化的体外研究

[1]Baht, G.S., Hunter, G.K., Goldberg, H.A., 2008. Bone sialoprotein-collagen interaction promotes hydroxyapatite nucleation. Matrix Biol., 27(7):600-608.

[2]Cai, Y., Tang, R., 2008. Calcium phosphate nanoparticles in biomineralization and biomaterials. J. Mater. Chem., 18:3775-3787.

[3]Carrilho, M.R., Tay, F.R., Pashley, D.H., et al., 2005. Mechanical stability of resin-dentin bond components. Dent. Mater., 21(3):232-241.

[4]Carrilho, M.R., Geraldeli, S., Tay, F., et al., 2007. In vivo preservation of the hybrid layer by chlorhexidine. J. Dent. Res., 86(6):529-533.

[5]Eanes, E., 2001. Amorphous calcium phosphate. In: Chow, L.C., Eanes, E.D. (Eds.), Octacalcium Phosphate. Monographs in Oral Science, Karger, Basel, Vol. 18, p.130-147.

[6]Fontana, M., Li, Y., Dunipace, A.J., et al., 1996. Measurement of enamel demineralization using microradiography and confocal microscopy. A correlation study. Caries Res., 30(5):317-325.

[7]Gajjeraman, S., Narayanan, K., Hao, J., et al., 2007. Matrix macromolecules in hard tissues control the nucleation and hierarchical assembly of hydroxyapatite. J. Biol. Chem., 282(2):1193-1204.

[8]Glimcher, M., 2006. Bone: nature of the calcium phosphate crystals and cellular, structural, and physical chemical mechanisms in their formation. Rev. Mineral. Geochem., 64(1):223-282.

[9]Gu, L.S., Huffman, B.P., Arola, D.D., et al., 2010. Changes in stiffness of resin-infiltrated demineralized dentin after remineralization by a bottom-up biomimetic approach. Acta Biomater., 6(4):1453-1461.

[10]Hashimoto, M., 2010. A review—micromorphological evidence of degradation in resin-dentin bonds and potential preventional solutions. J. Biomed. Mater. Res. B Appl. Biomater., 92(1):268-280.

[11]Hashimoto, M., Nakamura, K., Kaga, M., et al., 2008. Crystal growth by fluoridated adhesive resins. Dent. Mater., 24(4):457-463.

[12]He, G., Gajjeraman, S., Schultz, D., et al., 2005. Spatially and temporally controlled biomineralization is facilitated by interaction between self-assembled dentin matrix protein 1 and calcium phosphate nuclei in solution. Biochemistry, 44(49):16140-16148.

[13]Jardine, A.P., da Rosa, R.A., Santini, M.F., et al., 2016. The effect of final irrigation on the penetrability of an epoxy resin-based sealer into dentinal tubules: a confocal microscopy study. Clin. Oral Invest., 20(1):117-123.

[14]Kim, J., Vaughn, R.M., Gu, L., et al., 2010. Imperfect hybrid layers created by an aggressive one-step self-etch adhesive in primary dentin are amendable to biomimetic remineralization in vitro. J. Biomed. Mater. Res. A, 93(4):1225-1234.

[15]Kim, Y.K., Mai, S., Mazzoni, A., et al., 2010a. Biomimetic remineralization as a progressive dehydration mechanism of collagen matrices—implications in the aging of resin-dentin bonds. Acta Biomater., 6(9):3729-3739.

[16]Kim, Y.K., Gu, L.S., Bryan, T.E., et al., 2010b. Mineralisation of reconstituted collagen using polyvinylphosphonic acid/polyacrylic acid templating matrix protein analogues in the presence of calcium, phosphate and hydroxyl ions. Biomaterials, 31(25):6618-6627.

[17]Kinney, J.H., Habelitz, S., Marshall, S.J., et al., 2003. The importance of intrafibrillar mineralization of collagen on the mechanical properties of dentin. J. Dent. Res., 82(12):957-961.

[18]Kokubo, T., Kushitani, H., Sakka, S., et al., 1990. Solutions able to reproduce in vivo surface-structure changes in bioactive glass-ceramic A-W. J. Biomed. Mater. Res. A, 24(6):721-734.

[19]Li, H., Burrow, M.F., Tyas, M.J., 2000. Nanoleakage patterns of four dentin bonding systems. Dent. Mater., 16(1):48-56.

[20]Lin, J., Mehl, C., Yang, B., et al., 2010. Durability of four composite resin cements bonded to dentin under simulated pulpal pressure. Dent. Mater., 26(10):1001-1009.

[21]Lin, J., Zheng, W.Y., Liu, P.R., et al., 2014. Influence of casein phosphopeptide-amorphous calcium phosphate application, smear layer removal, and storage time on resin-dentin bonding. J. Zhejiang Univ.-Sci. B (Biomed. & Biotechnol.), 15(7):649-660.

[22]Liu, Y., Tjäderhane, L., Breschi, L., et al., 2011. Limitations in bonding to dentin and experimental strategies to prevent bond degradation. J. Dent. Res., 90(8):953-968.

[23]Luo, X.J., Yang, H.Y., Niu, L.N., et al., 2016. Translation of a solution-based biomineralization concept into a carrier-based delivery system via the use of expanded-pore mesoporous silica. Acta Biomater., 31(10):378-387.

[24]Mai, S., Kim, Y.K., Toledano, M., et al., 2009. Phosphoric acid esters cannot replace polyvinylphosphonic acid as phosphoprotein analogs in biomimetic remineralization of resin-bonded dentin. Dent. Mater., 25(10):1230-1239.

[25]Meyer, J.L., Weatherall, C.C., 1982. Amorphous to crystalline calcium phosphate phase transformation at elevated pH. J. Colloid Interface Sci., 89(1):257-267.

[26]Olszta, M.J., Odom, D.J., Douglas, E.P., et al., 2003. A new paradigm for biomineral formation: mineralization via an amorphous liquid-phase precursor. Connect. Tissue Res., 44(1):326-334.

[27]Pashley, D.H., Tay, F.R., Yiu, C., et al., 2004. Collagen degradation by host-derived enzymes during aging. J. Dent. Res., 83(3):216-221.

[28]Pashley, D.H., Tay, F.R., Carvalho, R.M., et al., 2007. From dry bonding to water-wet bonding to ethanol-wet bonding. A review of the interactions between dentin matrix and solvated resins using a macromodel of the hybrid layer. Am. J. Dent., 20(1):7-20.

[29]Pfarrer, A., Faller, R., Dusehner, H., 1996. Application of confocal laser scanning microscopy for studying remineralization and demineralization processes. J. Dent. Res., 1(75):543.

[30]Sauro, S., Osorio, R., Watson, T.F., et al., 2015. Influence of phosphoproteins’ biomimetic analogs on remineralization of mineral-depleted resin‒dentin interfaces created with ion-releasing resin-based systems. Dent. Mater., 31(7):759-777.

[31]Sidhu, S.K., Watson, T.F., 1998. Interfacial characteristics of resin-modified glass-ionomer materials: a study on fluid permeability using confocal fluorescence microscopy. J. Dent. Res., 77(9):1749-1759.

[32]Tay, F.R., Pashley, D.H., 2008. Guided tissue remineralisation of partially demineralised human dentine. Biomaterials, 29(8):1127-1137.

[33]Tay, F.R., Pashley, D.H., 2009. Biomimetic remineralization of resin-bonded acid-etched dentin. J. Dent. Res., 88(8):719-724.

[34]Tay, F.R., Pashley, D.H., Rueggeberg, F.A., et al., 2007. Calcium phosphate phase transformation produced by the interaction of the Portland cement component of white mineral trioxide aggregate with a phosphate-containing fluid. J. Endod., 33(11):1347-1351.

[35]Tjaderhane, L., Larjava, H., Sorsa, T., et al., 1998. The activation and function of host matrix metalloproteinases in dentin matrix breakdown in caries lesions. J. Dent. Res., 77(8):1622-1629.

[36]Toroian, D., Lim, J.E., Price, P.A., 2007. The size exclusion characteristics of type I collagen: implications for the role of noncollagenous bone constituents in mineralization. J. Biol. Chem., 282(31):22437-22447.

[37]Trebacz, H., Wojtowicz, K., 2005. Thermal stabilization of collagen molecules in bone tissue. Int. J. Biol. Macromol., 37(5):257-262.

[38]van der Veen, M.H., Tsuda, H., Arends, J., et al., 1996. Evaluation of sodium fluorescein for quantitative diagnosis of root caries. J. Dent. Res., 75(1):588-593.

[39]Wang, Y., Spencer, P., 2005. Interfacial chemistry of class II composite restoration: structure analysis. J. Biomed. Mater. Res. A, 75(3):580-587.

[40]Watson, T.F., Cook, R.J., Festy, F., et al., 2008. Optical imaging techniques for dental biomaterials interfaces. In: Curtis, R.V., Watson, T.F. (Eds.), Dental Biomaterials: Imaging, Testing and Modelling. Woodhead Publishing, Cambridge, Chapter 2, p.37-57.

[41]Xu, A.W., Ma, Y., Cölfen, H., 2007. Biomimetic mineralization. J. Mater. Chem., 17(5):415-449.

[42]Yang, B., Ludwig, K., Adelung, R., et al., 2006. Micro-tensile bond strength of three luting resins to human regional dentin. Dent. Mater., 22(1):45-56.

[43]Zhang, S., 2003. Fabrication of novel biomaterials through molecular self-assembly. Nat. Biotechnol., 21(10):1171-1178.

[44]Zhong, B., Peng, C., Wang, G., et al., 2015. Contemporary research findings on dentine remineralization. J. Tissue Eng. Regen. Med., 9(9):1004-1016.