Full Text:   <2993>

CLC number: Q786; Q939.13

On-line Access: 

Received: 2004-10-20

Revision Accepted: 2004-12-19

Crosschecked: 0000-00-00

Cited: 2

Clicked: 6699

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.464-469


Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor

Author(s):  ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan

Affiliation(s):  School of Life Science, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   lyq1962@yahoo.com

Key Words:  Streptomyces coelicolor, EYFP, Temporal and spatial expression, Pathway-specific gene redD

ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan. Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor[J]. Journal of Zhejiang University Science B, 2005, 6(6): 464-469.

@article{title="Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor",
author="ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor
%A ZHOU Li-hua
%A LI Yu-qin
%A LI Yong-quan
%A WU Dan
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 464-469
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0464

T1 - Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor
A1 - ZHOU Li-hua
A1 - LI Yu-qin
A1 - LI Yong-quan
A1 - WU Dan
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 464
EP - 469
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0464

Confocal laser scanning microscopy was used to observe the spatio-temporal expression of the pathway-specific gene redD during S. coelicolor cell cultivation. The corresponding mutant S. coelicolor lyqRY1522 carrying redD::EYFP in the chromosome was constructed. The temporal expression results of the fusion protein during submerged cultivation demonstrated that expression of redD began in the transition phase, continuing through the exponential growth phase to the stationary phase, and reached maximum in the stationary phase. On the other hand, redD was expressed only in substrate mycelia during solid-state culture, while aerial mycelia remained essentially non-fluorescent throughout culture. Results demonstrated that the expression pattern of redD coincides with that of the biosynthesis of the antibiotics during culture, revealing a direct correlation between the spatio-temporal distribution of regulatory gene expression and second metabolism.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Beavis, A.J., Kalejta, R.F., 1999. Simultaneous analysis of the cyan, yellow and green fluoresent proteins by flow cytometry using single-laser excitation at 458 nm. Cytometry, 37:68-73.

[2] Bierman, M., Logan, R., O’Brien, K., Seno, E.T., Rao, R.N., Schoner, B.E., 1992. Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene, 146:43-49.

[3] Brendan, P.C., Raphael, H.V., Stanley, F., 1996. FACS-optimized mutants of green fluorescence protein. Gene, 173:33-38.

[4] Fernandez-Moreno, M.A., Martinez, E., Caballero, J.L., Ichinose, K., Hopwood, D.A., Malpartida, F., 1994. DNA sequence and functions of the actVI region of the actinorhodin biosynthetic gene cluster of Streptomyces coelicolor A3(2). Journal of Biological Chemistry, 269(40):24854-24863.

[5] Gary, M.D., Stephen, C.W., 1999. Cell-Cell Signaling in Bacteria. American Society for Microbiology Press, Washington D.C., p.27-46.

[6] Hopwood, D.A., Chater, K.F., Bibb, M.J., 1995. Genetics of antibiotic production in Streptomyces coelicolor A3(2), a model streptomyces. Biotechnology, 28:65-102.

[7] Huang, J., Lih, C.J., Pan, K.H., Cohen, S.N., 2001. Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways in Streptomyces coelicolor using DNA microarrays. Genes & Development, 15:3183-3192.

[8] Kieser, T., Bibb, M.J., Chater, K.F., Hopwood, D.A., 2000. Practical Streptomyces Genetics. John Innes Foundation, Norwich, United Kingdom.

[9] Kyung, Y.S., Hu, W.S., Sherman, D.H., 2001. Analysis of temporal and spatial expression of the CcaR regulatory element in the cephamycin C biosynthetic pathway using green fluorescent protein. Molecular Microbiology, 40(3):530-541.

[10] Prasher, D.C., Eckenrode, V.K., Ward, W.W., Prendergast, F.G., Cormier, M.J., 1992. Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 111:229-233.

[11] Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Molecular Cloning, a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor Laboratory.

[12] Takano, E., Gramajo, H.C., Strauch, E., Andres, N., White, J., Bibb, M.J., 1992. Transcriptional regulation of the redD transcriptional activator gene accounts for growth-phase-dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2). Molecular Microbiology, 6(19):2797-2804.

[13] Yang, T.T., Sinai, P., Green, G., Kitts, P.A., Chen, Y.T., Lybarger, L., Chervenak, R., Patterson, G.H., Piston, D.W., Kain, S.R., 1998. Improved fluorescence and dual color detection with enhanced blue and green variants of the green fluorescent protein. Journal of Biological Chemistry, 273:8212-8216.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE