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CLC number: Q786; Q939.13

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Received: 2004-10-20

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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.6 P.464~469

10.1631/jzus.2005.B0464


Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor


Author(s):  ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan

Affiliation(s):  School of Life Science, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   lyq1962@yahoo.com

Key Words:  Streptomyces coelicolor, EYFP, Temporal and spatial expression, Pathway-specific gene redD


ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan. Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor[J]. Journal of Zhejiang University Science B, 2005, 6(6): 464~469.

@article{title="Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor",
author="ZHOU Li-hua, LI Yu-qin, LI Yong-quan, WU Dan",
journal="Journal of Zhejiang University Science B",
volume="6",
number="6",
pages="464~469",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0464"
}

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%T Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor
%A ZHOU Li-hua
%A LI Yu-qin
%A LI Yong-quan
%A WU Dan
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 6
%P 464~469
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0464

TY - JOUR
T1 - Spatio-temporal expression of the pathway–specific regulatory gene redD in S. coelicolor
A1 - ZHOU Li-hua
A1 - LI Yu-qin
A1 - LI Yong-quan
A1 - WU Dan
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 6
SP - 464
EP - 469
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0464


Abstract: 
Confocal laser scanning microscopy was used to observe the spatio-temporal expression of the pathway-specific gene redD during S. coelicolor cell cultivation. The corresponding mutant S. coelicolor lyqRY1522 carrying redD::EYFP in the chromosome was constructed. The temporal expression results of the fusion protein during submerged cultivation demonstrated that expression of redD began in the transition phase, continuing through the exponential growth phase to the stationary phase, and reached maximum in the stationary phase. On the other hand, redD was expressed only in substrate mycelia during solid-state culture, while aerial mycelia remained essentially non-fluorescent throughout culture. Results demonstrated that the expression pattern of redD coincides with that of the biosynthesis of the antibiotics during culture, revealing a direct correlation between the spatio-temporal distribution of regulatory gene expression and second metabolism.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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