JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2021 Vol.22 No.9 P.767-773

Effects of S-adenosylmethionine on production of secondary metabolites in Streptomyces diastatochromogenes 1628

Author(s): Yefeng HU, Juan WANG, Jie XU, Zheng MA, Andreas BECHTHOLD, Xiaoping YU
Affiliation(s): Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, China; more
Corresponding email(s): mazheng520@163.com
Key Words: S. diastatochromogenes 1628, SAM, MetK, Toyocamycin, Tetraene macrolides

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Abstract: Streptomyces are famous for their ability to synthesize a large number of bioactive compounds as secondary metabolites containing antibiotics, enzyme inhibitors, and other small molecules with potential physiological activity (Niu et al., 2016; Song et al., 2019; Yin et al., 2019). Secondary metabolites are produced by a multi-step reaction of a primary metabolite as a precursor (Liu et al., 2013; Li et al., 2021). Therefore, it is of great research significance to increase the overall synthesis level of antibiotics by increasing the amount of synthesis of precursors.

S-腺苷甲硫氨酸对淀粉酶产色链霉菌1628次级代谢产物产量的影响

目的：考察S-腺苷甲硫氨酸（SAM）对淀粉酶产色链霉菌（Streptomyces diastatochromogenes）1628次级代谢产物产量的影响。
创新点：SAM是甲基的重要供体，可为次级代谢产物提供前体，同时也可以作为信号增强次级代谢产物合成基因的表达。因此，本研究首次探究了SAM与S. diastatochromogenes1628次级代谢产物合成的相关性。
方法：以S. diastatochromogenes1628为对象，通过体外添加SAM以及在S. diastatochromogenes1628体内过表达、敲除和回补SAM编码基因metK_sd，考察S. diastatochromogenes1628次级代谢产物产量的变化。
结论：由于渗透性差，外源添加SAM对S. diastatochromogenes1628合成次级代谢产物（丰加霉素和三种四烯大环内酯类抗生素：四霉素A、四霉素P和四烯菌素B）没有影响；敲除、回补和过表达基因metK_sd证实增加胞内SAM可提高S. diastatochromogenes1628三种四烯大环内酯类抗生素的产量，且可促进四烯大环内酯类化合物合成关键基因的表达，而胞内SAM浓度的变化对丰加霉素的产量和及其关键基因的表达均无明显影响。

关键词：淀粉酶产色链霉菌1628；S-腺苷甲硫氨酸；MetK；丰加霉素；四烯大环内酯类

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