JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 1998 Vol.-1 No.-1 P.

Single-cell profiling of liver in offspring with intrauterine hyperglycemia reveals acute-phase response deficiency

Author(s): Jiahang MO^{1^}, Jing YAN^{1^}, Yi CHENG^{2^*}, Kaixuan DONG³, Tianyou WANG⁴, Jie LI¹, Meijun PAN¹, Guolian DING^1, ⁵, Hong ZHU^1, ⁵, Hefeng HUANG^1, ^5, ^6, ⁷
Affiliation(s): ¹Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan University, Shanghai 200090, China ²The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China ³Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China ⁴Department of Gynecology Oncology, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200090, China ⁵Shanghai Key Laboratory of Reproduction and Development, Shanghai 200032, China ⁶Key Laboratory of Reproductive Genetics (Ministry of Education), Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China; more
Corresponding email(s): Hong ZHU, Zhuhong-hope@163.com Hefeng HUANG, huanghefg@hotmail.com
Key Words: Intrauterine hyperglycemia (IHG), Single-cell RNA sequencing (sc-RNA seq), Liver, Myeloid cell, Inflammation, Acute-phase response

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Jiahang MO^{1^*}, Jing YAN^{1^*}, Yi CHENG^{2^*}, Kaixuan DONG³, Tianyou WANG⁴, Jie LI¹, Meijun PAN¹, Guolian DING^1,⁵, Hong ZHU^1,⁵, Hefeng HUANG^1,^5,^6,⁷. Single-cell profiling of liver in offspring with intrauterine hyperglycemia reveals acute-phase response deficiency[J]. Journal of Zhejiang University Science B, 1998, -1(-1): .

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author="Jiahang MO^{1^*}, Jing YAN^{1^*}, Yi CHENG^{2^*}, Kaixuan DONG³, Tianyou WANG⁴, Jie LI¹, Meijun PAN¹, Guolian DING^1,⁵, Hong ZHU^1,⁵, Hefeng HUANG^1,^5,^6,⁷",
journal="Journal of Zhejiang University Science B",
volume="-1",
number="-1",
pages="",
year="1998",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B2500269"
}

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%A Jiahang MO^{1^*}
%A Jing YAN^{1^*}
%A Yi CHENG^{2^*}
%A Kaixuan DONG³
%A Tianyou WANG⁴
%A Jie LI¹
%A Meijun PAN¹
%A Guolian DING^1
%A⁵
%A Hong ZHU^1
%A⁵
%A Hefeng HUANG^1
%A^5
%A^6
%A⁷
%J Journal of Zhejiang University SCIENCE B
%V -1
%N -1
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%@ 1673-1581
%D 1998
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B2500269

TY - JOUR
T1 - Single-cell profiling of liver in offspring with intrauterine hyperglycemia reveals acute-phase response deficiency
A1 - Jiahang MO^{1^*}
A1 - Jing YAN^{1^*}
A1 - Yi CHENG^{2^*}
A1 - Kaixuan DONG³
A1 - Tianyou WANG⁴
A1 - Jie LI¹
A1 - Meijun PAN¹
A1 - Guolian DING^{1
A1 -}⁵
A1 - Hong ZHU^{1
A1 -}⁵
A1 - Hefeng HUANG^{1
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A1 -}^{6
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J0 - Journal of Zhejiang University Science B
VL - -1
IS - -1
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%@ 1673-1581
Y1 - 1998
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B2500269

Abstract
Chinese Summary
Academic Network
Reviewer Comment

Abstract: Gestational diabetes mellitus (GDM) has long-term effects on offspring health. In the development of this chronic disease, inflammatory profiles play important roles. However, the assessment of GDM fetuses for their inflammatory status has been limited. Thus, single-cell RNA sequencing (scRNA-seq) is urgently needed to delineate the inflammatory characteristics of offspring with intrauterine hyperglycemia (IHG). Methods: A mouse IHG model was induced by streptozotocin (STZ). liver samples of offspring at the fetal and adult periods were collected for scRNA-seq analysis and further experiments. CytoTRACE and Monocle were used for the developmental analysis of fetal liver after dimension reduction and clustering. The CellChat algorithm was applied for the cellular interaction analysis of adult livers. A lipopolysaccharide (LPS)-induced sepsis model was built in adult offspring to evaluate the immune response. Subsequently, primary cell flow cytometry (FCM) of the bone marrow (BM) and liver was performed for myeloid lineage detection. Finally, the transcriptional and translational levels of acute-phase response cytokines were quantified. Results: Our study has mapped the single-cell profile of livers in offspring with IHG. Myeloid polarization was identified, accompanied by the developmental retardation of multiple cell lineages in IHG offspring. In adults, myeloid polarization was restored to some extent, while the proinflammatory potential of these myeloid cells seemed to be deeply molded. Adult offspring with IHG also showed deficiency in acute-phase response in the LPS-induced sepsis model. Conclusions: The single-cell profile of livers from offspring with IHG highlighted the comprehensive inflammatory status of the myeloid lineage and hinted acute-phase response deficiency in sepsis.

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