CLC number: R51
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
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ZHU Hai-hong, CHEN Zhi, ZHANG Ming-tai, DING Lie-ming. CONSTRUCTION AND IDENTIFICATION OF THE RECOMBINANT OF THE AAV VECTOR AND HUMAN INTERFERON-GAMMA[J]. Journal of Zhejiang University Science A, 2001, 2(4): 445-447.
@article{title="CONSTRUCTION AND IDENTIFICATION OF THE RECOMBINANT OF THE AAV VECTOR AND HUMAN INTERFERON-GAMMA",
author="ZHU Hai-hong, CHEN Zhi, ZHANG Ming-tai, DING Lie-ming",
journal="Journal of Zhejiang University Science A",
volume="2",
number="4",
pages="445-447",
year="2001",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2001.0445"
}
%0 Journal Article
%T CONSTRUCTION AND IDENTIFICATION OF THE RECOMBINANT OF THE AAV VECTOR AND HUMAN INTERFERON-GAMMA
%A ZHU Hai-hong
%A CHEN Zhi
%A ZHANG Ming-tai
%A DING Lie-ming
%J Journal of Zhejiang University SCIENCE A
%V 2
%N 4
%P 445-447
%@ 1869-1951
%D 2001
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2001.0445
TY - JOUR
T1 - CONSTRUCTION AND IDENTIFICATION OF THE RECOMBINANT OF THE AAV VECTOR AND HUMAN INTERFERON-GAMMA
A1 - ZHU Hai-hong
A1 - CHEN Zhi
A1 - ZHANG Ming-tai
A1 - DING Lie-ming
J0 - Journal of Zhejiang University Science A
VL - 2
IS - 4
SP - 445
EP - 447
%@ 1869-1951
Y1 - 2001
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2001.0445
Abstract: To construct and identify further a recombinant of adeno-associated virus and interferon-gamma for gene therapy, the full-length IFN-γcDNA containing signal peptide was amplified by PCR, and then cloned into the pUC18. After screening, the fragment from the positive clone was then subcloned into pwpl9. After the correct recombinant was identified by digestion with SacI and BamHI, it was transfected into lymphocyte cell line H9 mediated by calcium phosphate, and the expression of IFN-γ was detected by RT-PCR and ELISA. The result showed that the IFN-γ were expressed in the H9 cells transfected with pwp/IFN-γ. The so constructed recombinant plasmid pwpl9/IFN-γ containing the full-length IFN-γ gene was expressed in mammalian cells.
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