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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.4 P.324~330

10.1631/jzus.2006.B0324


Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis


Author(s):  Sun Huai-Chang, Xue Fang-Ming, Qian Ke, Fang Hao-Xia, Qiu Hua-Lei, Zhang Xin-Yu, Yin Zhao-Hua

Affiliation(s):  School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China

Corresponding email(s):   sun78564@hotmail.com

Key Words:  Human lysozyme cDNA, Mammary-specific vector, Expression, Mastitis, Gene therapy


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Sun Huai-Chang, Xue Fang-Ming, Qian Ke, Fang Hao-Xia, Qiu Hua-Lei, Zhang Xin-Yu, Yin Zhao-Hua. Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis[J]. Journal of Zhejiang University Science B, 2006, 7(4): 324~330.

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author="Sun Huai-Chang, Xue Fang-Ming, Qian Ke, Fang Hao-Xia, Qiu Hua-Lei, Zhang Xin-Yu, Yin Zhao-Hua",
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year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0324"
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%T Intramammary expression and therapeutic effect of a human lysozyme-expressing vector for treating bovine mastitis
%A Sun Huai-Chang
%A Xue Fang-Ming
%A Qian Ke
%A Fang Hao-Xia
%A Qiu Hua-Lei
%A Zhang Xin-Yu
%A Yin Zhao-Hua
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A1 - Sun Huai-Chang
A1 - Xue Fang-Ming
A1 - Qian Ke
A1 - Fang Hao-Xia
A1 - Qiu Hua-Lei
A1 - Zhang Xin-Yu
A1 - Yin Zhao-Hua
J0 - Journal of Zhejiang University Science B
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%@ 1673-1581
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DOI - 10.1631/jzus.2006.B0324


Abstract: 
To develop a gene therapy strategy for treating bovine mastitis, a new mammary-specific vector containing human lysozyme (hLYZ) cDNA and kanamycin resistance gene was constructed for intramammary expression and clinical studies. After one time acupuncture or intracisternal infusion of healthy cows with 400 μg of the p215C3LYZ vector, over 2.0 μg/ml of rhLYZ could be detected by enzymatic assay for about 3 weeks in the milk samples. Western blotting showed that rhLYZ secreted into milk samples from the vector-injected cows had molecular weight similar to that of the natural hLYZ in human colostrums. Twenty days after the primary injection, the quarters were re-injected with the same vector by quarter acupuncture and even higher concentrations of rhLYZ could be detected. Indirect competitive ELISA of milk samples showed that the vector injection did not induce detectable humoral immune response against hLYZ. Clinical studies showed that twice acupuncture of quarters with the p215C3LYZ vector had overt therapeutic effect on clinical and subclinical mastitis previously treated with antibiotics, including disappearance of clinical symptoms and relatively high microbiological cure rates. These data provide a solid rationale for using the vector to develop gene therapy for treating bovine mastitis.

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vivek bahuguna@ndrikarnal<vn1bahuguna@gmail.com>

2012-05-25 12:15:07

nice article.

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