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Journal of Zhejiang University SCIENCE B 2008 Vol.9 No.5 P.351~355

http://doi.org/10.1631/jzus.B0710596


Transient expression of chicken alpha interferon gene in lettuce


Author(s):  Li SONG, De-gang ZHAO, Yong-jun WU, Yi LI

Affiliation(s):  Guizhou Key Laboratory of Ago-Bioengineering, Guizhou University, Guiyang 550025, China; more

Corresponding email(s):   dgzhao@gzu.edu.cn

Key Words:  Chicken alpha interferon (ChIFN-&alpha, ), Expression, Transgenic lettuce, Bioactivity


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Li SONG, De-gang ZHAO, Yong-jun WU, Yi LI. Transient expression of chicken alpha interferon gene in lettuce[J]. Journal of Zhejiang University Science B, 2008, 9(5): 351~355.

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author="Li SONG, De-gang ZHAO, Yong-jun WU, Yi LI",
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%DOI 10.1631/jzus.B0710596

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T1 - Transient expression of chicken alpha interferon gene in lettuce
A1 - Li SONG
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A1 - Yi LI
J0 - Journal of Zhejiang University Science B
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B0710596


Abstract: 
We investigated the possibility of producing chicken alpha interferon (ChIFN-&alpha;) in transgenic plants. The cDNA encoding ChIFN-α was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF). The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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