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Abstract: We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants. The cDNA encoding ChIFN-α was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system. The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays. Recombinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF). The results demonstrate that biologically active avian cytokine with potential pharmaceutical applications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.

[1] Ahmed, M., McKenzie, M.O., Puckett, S., Hojnacki, M., Poliquin, L., Lyles, D.S., 2003. Ability of the matrix protein of vesicular stomatitis virus to suppress beta interferon gene expression is genetically correlated with the inhibition of host RNA and protein synthesis. Journal of Virology, 77(8):4646-4657.

[2] Arazi, T., Slutsky, S.G., Shiboleth, Y.M., Wang, Y.Z., Rubinstein, M., Barak, S., Yang, J., Gal-On, A., 2001. Engineering zucchini yellow mosaic potyvirus as a non-pathogenic vector for expression of heterologous proteins in cucurbits. Journal of Biotechnology, 87(1):67-82.

[3] Boston, R.S., Viitanen, P.V., Vierling, E., 1996. Molecular chaperones and protein folding in plants. Plant Molecular Biology, 32(1-2):191-222.

[4] Cauthen, A.N., Swayne, D.E., Sekellick, M.J., Marcus, P.I., Suarez, D.L., 2007. Amelioration of influenza virus pathogenesis in chickens attributed to the enhanced interferon-inducing capacity of a virus with a truncated NS1 gene. Journal of Virology, 81(4):1838-1847.

[5] Crouch, M.L., Tenbarge, K.M., Simon, A.E., Ferl, R., 1983. cDNA clones for Brassica napus seed storage proteins: evidence from nucleotide sequence analysis that both subunits of napin are cleaved from a precursor polypeptide. Journal of Molecular and Applied Genetics, 2(3):273-283.

[6] Denecke, J., Carlsson, L.E., Vidal, S., Hoglund, A.S., Ek, B., van Zeijl, M.J., Sinjorgo, K.M., Palva, E.T., 1995. The tobacco homolog of mammalian calreticulin is present in protein complexes in vivo. Plant Cell, 7(4):391-406.

[7] Fredericksen, B.L., Keller, B.C., Fornek, J., Katze, M.G., Gale, M.Jr., 2008. Establishment and maintenance of the innate antiviral response to west nile virus involves both RIG-I and MDA5 signaling through IPS-1. Journal of Virology, 82(2):609-616.

[8] Habib, M., Hussain, I., Fang, W.H., Rajput, Z.I., Yang, Z.Z., Irshad, H., 2006. Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks. Journal of Zhejiang University SCIENCE B, 7(8):660-664.

[9] Jarosinski, K.W., Jia, W., Sekellick, M.J., Marcus, P.I., Schat, K.A., 2001. Cellular responses in chickens treated with IFN-alpha orally or inoculated with recombinant Marek’s disease virus expressing IFN-alpha. Journal of Interferon and Cytokine Research, 21(5):287-296.

[10] Jourdain, E., Gauthier-Clerc, M., Bicout, D.J., Sabatier, P., 2007. Bird migration routes and risk for pathogen dispersion into western Mediterranean wetlands. Emerging Infectious Diseases, 13(3):365-372.

[11] Khoudi, H., Laberge, S., Ferullo, J.M., Bazin, R., Darveau, A., Castonguay, Y., Allard, G., Lemieux, R., Vézina, L.P., 1999. Production of a diagnostic monoclonal antibody in perennial alfalfa plants. Biotechnology and Bioengineering, 64(2):135-143.

[12] Kochs, G., García-Sastre, A., Martínez-Sobrido, L., 2007. Multiple anti-interferon actions of the influenza A virus NS1 protein. Journal of Virology, 81(13):7011-7021.

[13] Li, J., Chen, M., Liu, X.W., Zhang, H.C., Shen, F.F., George, P.W., 2007. Transient expression of an active human interferon-beta in lettuce. Scientia Horticulturae, 112(3):258-265.

[14] Marcus, P.I., van der Heide, L., Sekellick, M.J., 1999. Interferon action on avian viruses. I. Oral administration of chicken interferon-alpha ameliorates new castle disease. Journal of Interferon and Cytokine Research, 19(8):881-885.

[15] Mo, C.W., Cao, Y.C., Lim, B.L., 2001. The in vivo and in vitro effects of chicken interferon alpha on infectious bursal disease virus and new castle disease virus infection. Avian Diseases, 45(2):389-399.

[16] Negrouk, V., Eisner, G., Lee, H.I., Han, K., Taylor, D., Wong, H.C., 2005. Highly efficient transient expression of functional recombinant antibodies in lettuce. Plant Science, 169(2):433-438.

[17] Ohya, K., Matsumura, T., Ohashi, K., Onuma, M., Sugimoto, C., 2001. Expression of two subtypes of human IFN-α in transgenic potato plants. Journal of Interferon and Cytokine Research, 21(8):595-602.

[18] Ohya, K., Matsumura, T., Itchoda, N., Ohashi, K., Onuma, M., Sugimoto, C., 2005. Ability of orally administered IFN-α-containing transgenic potato extracts to inhibit Listeria monocytogenes infection. Journal of Interferon and Cytokine Research, 25(8):459-466.

[19] Pei, J., Sekellick, M.J., Marcus, P.I., Choi, I.S., Collisson, E.W., 2001. Chicken interferon type I inhibits infectious bronchitis virus replication and associated respiratory illness. Journal of Interferon and Cytokine Research, 21(12):1071-1077.

[20] Plachý, J., Weining, K.C., Kremmer, E., Puehler, F., Hala, K., Kaspers, B., Staeheli, P., 1999. Proctive effects of type I and type II interferons toward Rous sarcoma virus-induced tumors in chickens. Virology, 256(1):85-91.

[21] Rajput, Z.I., Xiao, C.W., Hu, S.H., Arijo, A.G., Soomro, N.M., 2007. Improvement of the efficacy of influenza vaccination (H5N1) in chicken by using extract of Cochinchina momordica seed (ECMS). Journal of Zhejiang University SCIENCE B, 8(5):331-337.

[22] Ruttanapumma, R., Nakamura, M., Takehara, K., 2005. High level expression of recombinant chicken interferon-alpha using baculovirus. The Journal of Veterinary Medical Science, 67(1):25-28.

[23] Sawahel, W.A., 2002. The production of transgenic potato plants expressing human alpha-interferon using lipofectin-mediated transformation. Cellular and Molecular Biology Letters, 7(1):19-29.

[24] Schultz, U., Kaspers, B., Rinderle, C., Sekellick, M.J., Marcus, P.I., Staeheli, P., 1995a. Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity. European Journal of Immunology, 25(3):847-851.

[25] Schultz, U., Rinderle, C., Sekellick, M.J., Marcus, P.I., Staeheli, P., 1995b. Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active. European Journal of Biochemistry, 229(1):73-76.

[26] Sekellick, M.J., Ferrandino, A.F., Hopkins, D.A., Marcus, P.I., 1994. Chicken interferon gene: cloning, expression, and analysis. Journal of Interferon Research, 14(2):71-79.

[27] Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Monecke, M., Schillberg, S., Fischer, R., 1999. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proceedings of the National Academy of Sciences, 96(20):11128-11133.

[28] Wei, Q., Peng, G.Q., Jin, M.L., Zhu, Y.D., Zhou, H.B., Guo, H.Y., Chen, H.C., 2006. Cloning, prokaryotic expression of chicken interferon-alpha gene and study on antiviral effect of recombinant chicken interferon-alpha. Sheng Wu Gong Cheng Xue Bao, 22(5):737-743 (in Chinese).

[29] Witter, R.L., 1997. Increased virulence of Marek’s disease virus field isolates. Avian Diseases, 41(1):149-163.

[30] Xia, C., Liu, J., Wu, Z.G., Lin, C.Y., Wang, M., 2004. The interferon-α genes from three chicken lines and its effects on H9N2 influenza viruses. Animal Biotechnology, 15(1):77-88.

[31] Zhu, Q., Yang, H., Chen, W., Cao, W., Zhong, G., Jiao, P., Deng, G., Yu, K., Yang, C., Bu, Z., Kawaoka, Y., Chen, H., 2008. A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens. Journal of Virology, 82(1):220-228.

[32] Zhu, Z., Hughes, K.W., Huang, L., Sun, B.L., Liu, C.M., Li, Y., 1994. Expression of human α-interferon cDNA in transgenic rice plants. Plant Cell Tissue and Organ Culture, 36(2):197-204.