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On-line Access: 2018-06-04

Received: 2017-05-03

Revision Accepted: 2017-08-09

Crosschecked: 2018-05-14

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 ORCID:

Hui Li

https://orcid.org/0000-0002-7151-5912

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Journal of Zhejiang University SCIENCE B 2018 Vol.19 No.6 P.445-457

10.1631/jzus.B1700238


Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content


Author(s):  Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li

Affiliation(s):  Key Laboratory of Protected Horticulture of Liaoning Province / Collaborative Innovation Center of Protected Vegetable Surround Bohai Gulf Region / Horticulture Department, College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China

Corresponding email(s):   2671329915@qq.com, tianlaili@126.com

Key Words:  SlYABBY2b, Gibberellin, Gibberellin-deficient mutants, Ovary locule number, Transgenic plant, Gene expression, Tomato


Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li. Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content[J]. Journal of Zhejiang University Science B, 2018, 19(6): 445-457.

@article{title="Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content",
author="Hui Li, Mei-hua Sun, Ming-fang Qi, Jiao Xing, Tao Xu, Han-ting Liu, Tian-lai Li",
journal="Journal of Zhejiang University Science B",
volume="19",
number="6",
pages="445-457",
year="2018",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1700238"
}

%0 Journal Article
%T Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content
%A Hui Li
%A Mei-hua Sun
%A Ming-fang Qi
%A Jiao Xing
%A Tao Xu
%A Han-ting Liu
%A Tian-lai Li
%J Journal of Zhejiang University SCIENCE B
%V 19
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%P 445-457
%@ 1673-1581
%D 2018
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1700238

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T1 - Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content
A1 - Hui Li
A1 - Mei-hua Sun
A1 - Ming-fang Qi
A1 - Jiao Xing
A1 - Tao Xu
A1 - Han-ting Liu
A1 - Tian-lai Li
J0 - Journal of Zhejiang University Science B
VL - 19
IS - 6
SP - 445
EP - 457
%@ 1673-1581
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PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1700238


Abstract: 
tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellin-deficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.

SlYABBY2b基因对番茄果实心室数和内源赤霉 素含量的影响

目的:探讨赤霉素对SlYABBY2b基因调控番茄心室形成过程中的作用,为进一步研究番茄畸形果发生机理提供了理论基础.
创新点:首次明确了SlYABBY2b基因与赤霉素的关系,且筛选出SlYABBY2b调控赤霉素合成的关键基因GA20ox1GA3ox2GA2ox1GA2ox5.
方法:利用Gateway技术法构建SlYABBY2b基因超表达和沉默载体,并通过农杆菌介导转化法获得转基因植株.用酶联免疫吸附测定(ELISA)试剂盒检测转基因植株中赤霉素的含量,用实时荧光定量分析(qRT-PCR)赤霉素突变体中SlYABBY2b基因表达水平和SlYABBY2b转基因植株中赤霉素相关基因的转录水平.
结论:本实验中赤霉素突变体中SlYABBY2b基因表达量显示,赤霉素短缺导致番茄植株体内SlYABBY2b基因的升高.ELISA实验结果显示,SlYABBY2b基因也能够反馈调控赤霉素的合成.qRT-PCR结果显示,SlYABBY2b基因抑制GA20ox1GA3ox2基因的表达,促进GA2ox1GA2ox5基因的表达.综上所述,研究结果明确了SlYABBY2b基因位于赤霉素信号转导的途径上,反馈调节赤霉素的合成,感受外源赤霉素的信号,从而进一步调控番茄心室的形成.

关键词:SlYABBY2b基因;赤霉素;赤霉素突变体;子房心室;转基因植株;基因表达;番茄

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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[51]Table S2 RT-PCR primers used to amplify gene-specific regions

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