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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.2 P.133~136

http://doi.org/10.1631/jzus.2004.0133


Enhancing rice resistance to fungal pathogensby transformation with cell wall degrading enzyme genes from Trichoderma atroviride


Author(s):  LIU Mei, SUN Zong-xiu, ZHU Jie, XU Tong, HARMAN Gary E., LORITO Matteo

Affiliation(s):  College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   xutong@zju.edu.cn

Key Words:  Agrobacterium-mediated transformation, Rice sheath blight, Rice blast, Trichoderma atroviride, Chitinase, Glucanase


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LIU Mei, SUN Zong-xiu, ZHU Jie, XU Tong, HARMAN Gary E., LORITO Matteo. Enhancing rice resistance to fungal pathogensby transformation with cell wall degrading enzyme genes from Trichoderma atroviride[J]. Journal of Zhejiang University Science A, 2004, 5(2): 133~136.

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author="LIU Mei, SUN Zong-xiu, ZHU Jie, XU Tong, HARMAN Gary E., LORITO Matteo",
journal="Journal of Zhejiang University Science A",
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doi="10.1631/jzus.2004.0133"
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%A LIU Mei
%A SUN Zong-xiu
%A ZHU Jie
%A XU Tong
%A HARMAN Gary E.
%A LORITO Matteo
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T1 - Enhancing rice resistance to fungal pathogensby transformation with cell wall degrading enzyme genes from Trichoderma atroviride
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A1 - SUN Zong-xiu
A1 - ZHU Jie
A1 - XU Tong
A1 - HARMAN Gary E.
A1 - LORITO Matteo
J0 - Journal of Zhejiang University Science A
VL - 5
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0133


Abstract: 
Three genes encoding for fungal cell wall degrading enzymes (CWDEs), ech42, nag70 and gluc78 from the biocontrol fungus Trichoderma atroviride were inserted into the binary vector pCAMBIA1305.2 singly and in all possible combinations and transformed to rice plants. More than 1800 independently regenerated plantlets in seven different populations (for each of the three genes and each of the four gene combinations) were obtained. The ech42 gene encoding for an endochitinase increased resistance to sheath blight caused by Rhizoctonia solani, while the exochitinase-encoding gene, nag70, had lesser effect. The expression level of endochitinase but exochitinase was correlated with disease resistance. Nevertheless, exochitinase enhanced the effect of endochitinase on disease resistance when the two genes co-expressed in transgenics. Resistance to Magnaporthe grisea was found in all kinds of regenerated plants including that with single gluc78. A few lines expressing either ech42 or nag70 gene were immune to the disease. Transgenic plants are being tested to further evaluate disease resistance at field level. This is the first report of multiple of expression of genes encoding CWDEs from Trichoderma atroviride that result in resistance to blast and sheath blight in rice.

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Reference

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[2] Bolar, J.P., Norelli, J.L., Harman, G.E., Brown, S.K., Aldwinckle, H.S., 2001. Synergistic activity of endochitinase and exochitinase fromTrichoderma atroviride(T. harzianum) against the pathogenic fungus (Venturia inaequalis) in transgenic apple plants.Transgenic Res,1067:1-11.

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[5] Lorito, M., Del Sorbo, G., Scala, F., 2002. Molecular Approaches for Increasing Plant Resistance to Biotic and Abiotic Stresses.In: Breeding for Ornamentals: Classical and Molecular Approaches. A. Vainstein ed., Kluwer Academic Publishers, the Netherlands, p.197-218.

[6] Lorito, M., Hayes, C.K., Di Pietro, A., Woo, S.L., Harman, G.E., 1994. Purification, characterization and synergistic activity of a glucan 1,3-β-glucosidase and an N-acetyl-β-glucosaminidase fromTrichoderma harzianum. Phytopathology, 84:398-405.

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