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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.4 P.390~399

http://doi.org/10.1631/jzus.2004.0390


Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice


Author(s):  JIN Wei-zheng, WANG Shao-min, XU Min, DUAN Rui-jun, WU Ping

Affiliation(s):  The State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences,Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   docpwu@cls.zju.edu.cn

Key Words:  Ac/Ds, Enhancer trap, Excision, Gene trap, Reinsertion, Rice (Oryza sativa L.)


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JIN Wei-zheng, WANG Shao-min, XU Min, DUAN Rui-jun, WU Ping. Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice[J]. Journal of Zhejiang University Science A, 2004, 5(4): 390~399.

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author="JIN Wei-zheng, WANG Shao-min, XU Min, DUAN Rui-jun, WU Ping",
journal="Journal of Zhejiang University Science A",
volume="5",
number="4",
pages="390~399",
year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0390"
}

%0 Journal Article
%T Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice
%A JIN Wei-zheng
%A WANG Shao-min
%A XU Min
%A DUAN Rui-jun
%A WU Ping
%J Journal of Zhejiang University SCIENCE A
%V 5
%N 4
%P 390~399
%@ 1869-1951
%D 2004
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0390

TY - JOUR
T1 - Characterization of enhancer trap and gene trap harboring Ac/Ds transposon in transgenic rice
A1 - JIN Wei-zheng
A1 - WANG Shao-min
A1 - XU Min
A1 - DUAN Rui-jun
A1 - WU Ping
J0 - Journal of Zhejiang University Science A
VL - 5
IS - 4
SP - 390
EP - 399
%@ 1869-1951
Y1 - 2004
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2004.0390


Abstract: 
Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-element ac/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision of Ds element was examined by PCR amplification. The excision frequency of Ds element varied from 0% to 40% among 20 F2 populations derived from 11 different Ds parents. Southern blot analysis revealed that more than 70% of excised Ds elements reinserted into rice genome and above 70% of the reinserted Ds elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The GUS positive lines will be useful for identifying gene function in rice.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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