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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.10 P.731-739


Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice

Author(s):  Xin-chun ZHANG, Xin YU, Hui-juan ZHANG, Feng-ming SONG

Affiliation(s):  State Key Laboratory for Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   fmsong@zju.edu.cn

Key Words:  Rice, Oryza sativa L., Magnaporthe oryzae, Defense response, OsBIABP1, AMP-binding protein

Xin-chun ZHANG, Xin YU, Hui-juan ZHANG, Feng-ming SONG. Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice[J]. Journal of Zhejiang University Science B, 2009, 10(10): 731-739.

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author="Xin-chun ZHANG, Xin YU, Hui-juan ZHANG, Feng-ming SONG",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice
%A Xin-chun ZHANG
%A Xin YU
%A Hui-juan ZHANG
%A Feng-ming SONG
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 10
%P 731-739
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0920042

T1 - Molecular characterization of a defense-related AMP-binding protein gene, OsBIABP1, from rice
A1 - Xin-chun ZHANG
A1 - Xin YU
A1 - Hui-juan ZHANG
A1 - Feng-ming SONG
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 10
SP - 731
EP - 739
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0920042

We cloned and characterized a rice gene OsBIABP1 encoding an AMP-binding protein. The full-length cDNA of OsBIABP1 is 1912-bp long and is predicted to encode a 558-aa protein. OsBIABP1 contains a typical AMP-binding signature motif and shows high similarity to members of AMP-binding protein family. OsBIABP1 is expressed in stems, leaves and flowers of rice plants, but is not expressed, or expressed at a very low level, in rice roots. The expression of OsBIABP1 was induced by some defense-related signal molecules, e.g., salicylic acid (SA), benzothiadiazole, jasmonic acid (JA), and 1-amino cyclopropane-1-carboxylic acid, which mediate SA- and JA/ethylene (ET)-dependent defense signaling pathways, respectively. Furthermore, the expression of OsBIABP1 is activated by the infection of Magnaporthe oryzae, and the induced expression is quicker and stronger during early stages of pathogenesis in incompatible interaction than that in compatible interaction between rice and M. oryzae. Our results suggest that OsBIABP1 may be a defense-related AMP-binding protein that is involved in the regulation of defense response through SA and/or JA/ET signaling pathways.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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