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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.6 P.668-675


Interaction of catechins with aluminum in vitro

Author(s):  TANG De-song, SHEN Sheng-rong, CHEN Xun, ZHANG Yu-yan, XU Chong-yang

Affiliation(s):  Department of Tea Sciences, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   shrshen@zju.edu.cn

Key Words:  Aluminum, Tea polyphenols, Catechins, Complex, Kow

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TANG De-song, SHEN Sheng-rong, CHEN Xun, ZHANG Yu-yan, XU Chong-yang. Interaction of catechins with aluminum in vitro[J]. Journal of Zhejiang University Science A, 2004, 5(6): 668-675.

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T1 - Interaction of catechins with aluminum in vitro
A1 - TANG De-song
A1 - SHEN Sheng-rong
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A1 - ZHANG Yu-yan
A1 - XU Chong-yang
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DOI - 10.1631/jzus.2004.0668

Tea is one of the most popular beverages, consumed by over two thirds of the world's population; but the aluminum accumulation property of tea plant is becoming the focus of many researches because of aluminum's known adverse effect on human health. Investigation of the interactions of catechins with Al3+ showed that during the interaction of catechins with Al3+, the UV-vis spectrum of catechins was changed. Absorption of EGCG at 274 nm decreased and increased at 322 nm; EC and C's at 278 nm changed little. The ratio of Al3+ to EGCG was 1:1 in pH 5.0 buffer solution; in pH 6.2 buffer solution, the ratio in the Al-EGCG complex was 1:1. Interestingly, while the ratio reached to over 2, after the complex of Al-EGCG started polymerization, the ratio in the polymer was 2:1. In pH 6.2 buffer solution, the complex behavior of C with Al3+ was the same as that of EGCG, with a little difference for EC. When the ratio of Al3+ to EC was <1, the complex in ratio was 1:2, but, the complex polymerized when the ratio of Al3+ to EC was >1. It was found that the ratio of Al3+ to EC in the polymer was 1:1. Polymerization of Al-catechin complexes might reduce aluminum absorption in the intestine. kow value was also employed to study the properties of aluminum species in tea infusion (at gastric and intestine pH condition) and the effect of catechins and tea polyphenols on kow in buffer solution. Results showed that kowvalue rose much higher at the intestine pH than at the gastric pH. tea polyphenols and catechins could greatly reduce aluminum kow value in acetic buffer, indicating that these compounds may reduce aluminum absorption during tea intake.

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[1] Berthon, G., 2002. Aluminum speciation in relation to aluminun bioavailability, metabolism and toxity.Coordination Chemistry Reviews,228:319-341.

[2] Deng, M.E., 2002. Spectrophotometric determination of aluminum with bromopyroga11o1red.Metallurgical Analysis,22(3):60-61.

[3] Emiko, O., Tomoko, S., Masahiko, K., 1999. Complexation of aluminum with ()-epigallocatechin gallate studied by spectrophotometry.Journal of Inorganic Biochemisty,73:31-34.

[4] Flaten, T.P., 2002. Aluminium in tea concentrations, speciation and bioavailability.Coordination Chemistry Reviews,228:385-395.

[5] He, Y.B., Zhao, Y.H., Wang L.S., 1994. Determination ofn-octanol/water partition coefficient of organic compound.Environmental Chemistry,131:95-197.

[6] Huang, J., Yu, G., Zhang, P.Y., 2002. Predicting the lgKowof PCDFs using single-phenyl-ring cl-substitution pattern index.Environmental Science Research,15:1-5.

[7] Hynes, M.J., Coinceanainn, M.., 2001. The kinetics and mechanisms of the reaction of iron(III) with gallic acid, gallic acid methyl ester and catechin.Journal of Biochemistry,85:131-142.

[8] Inoue, M.B., Inoue, M., Fernando, Q., Valcic, S., Timmermann, B. N., 2002. Potentiometric and1H NMR studies of complexation of Al3+with ()-epigallocatechin gallate, a major active constituent of green tea.Journal of Inorganic Biochemistry,88:7-13.

[9] Midori, K., Tamiyoshi, S., Kinuyo, N., Masaaki, T., 2001. Effects of pH and metal ions on antioxidative actives of catechins.Biosci. Biotechnol. Biochem.,65:126-132.

[10] Yokel, R.A., 2002. Aluminum chelation principles and recent advances.Coordination Chemistry Reviews,228:97-113.

[11] Yu, G., Xu, X.B., 1993. Determination of the 1-octanol/ water partition coefficients of nitro-pan.Environmental Chemistry,12:299-303.

[12] Zhong, G.F., 1998, Physiology, the Third Edition. People's Medical Publishing House, Beijing, p.110, 117.

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