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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.656~663

10.1631/jzus.2005.B0656


Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase


Author(s):  YANG Hua, ZHENG Shu, MEIJER Laurent, LI Shi-min, LECLERC Sophie, YU Lin-lin, CHENG Jin-quan, ZHANG Su-zhan

Affiliation(s):  Cancer Institute, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   YangH@moffitt.usf.edu, zhengshu@zju.edu.cn

Key Words:  Chinese medicinal herbs, Cdc25 phosphatase inhibition, Cell cycle, Anti-tumor activity


YANG Hua, ZHENG Shu, MEIJER Laurent, LI Shi-min, LECLERC Sophie, YU Lin-lin, CHENG Jin-quan, ZHANG Su-zhan. Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase[J]. Journal of Zhejiang University Science B, 2005, 6(7): 656~663.

@article{title="Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase",
author="YANG Hua, ZHENG Shu, MEIJER Laurent, LI Shi-min, LECLERC Sophie, YU Lin-lin, CHENG Jin-quan, ZHANG Su-zhan",
journal="Journal of Zhejiang University Science B",
volume="6",
number="7",
pages="656~663",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0656"
}

%0 Journal Article
%T Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase
%A YANG Hua
%A ZHENG Shu
%A MEIJER Laurent
%A LI Shi-min
%A LECLERC Sophie
%A YU Lin-lin
%A CHENG Jin-quan
%A ZHANG Su-zhan
%J Journal of Zhejiang University SCIENCE B
%V 6
%N 7
%P 656~663
%@ 1673-1581
%D 2005
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0656

TY - JOUR
T1 - Screening the active constituents of Chinese medicinal herbs as potent inhibitors of Cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2 kinase
A1 - YANG Hua
A1 - ZHENG Shu
A1 - MEIJER Laurent
A1 - LI Shi-min
A1 - LECLERC Sophie
A1 - YU Lin-lin
A1 - CHENG Jin-quan
A1 - ZHANG Su-zhan
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 656
EP - 663
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0656


Abstract: 
Objective: To screen and evaluate the active constituents of chinese medicinal herbs as potent inhibitors of Cdc25 phosphatase. Methods: The affinity chromatography purified glutashione-S-transferase/Cdc25A phosphatase fusion protein and Cdc2/cyclin B from the extracts of starfish M phase oocytes are used as the cell cycle-specific targets for screening the antimitotic constituents. We tested 9 extracts isolated from the chinese medicinal herbs and vegetables including the agents currently used in cancer treatment by measuring the inhibition of Cdc25A phosphatase and Cdc2 kinase activity. The antitumor activity of the extracts was also evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay and flow cytometry. Results: Cdc25A inhibitory activity and antitumor activity are detected in the extracts isolated from three chinese medicinal herbs Agrimona pilosa; Herba solani lyrati; Galla chinesis. Conclusion: We found three extracts isolated from chinese medicinal herbs have potential inhibitory activity of Cdc25 phosphatase using a highly specific mechanism-based screen assay for antimitotic drug discovery.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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