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Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.7 P.1157~1163

http://doi.org/10.1631/jzus.2007.A1157


Effects of Hg and Cu on the activities of soil acid phosphatase


Author(s):  XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping

Affiliation(s):  Institute of Environmental Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   xudongmei@zju.edu.cn

Key Words:  Acid phosphatase (ACPase), Activity, Heavy metal, Kinetic property, Soil


XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping. Effects of Hg and Cu on the activities of soil acid phosphatase[J]. Journal of Zhejiang University Science A, 2007, 8(7): 1157~1163.

@article{title="Effects of Hg and Cu on the activities of soil acid phosphatase",
author="XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping",
journal="Journal of Zhejiang University Science A",
volume="8",
number="7",
pages="1157~1163",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.A1157"
}

%0 Journal Article
%T Effects of Hg and Cu on the activities of soil acid phosphatase
%A XU Dong-mei
%A CHEN Bo
%A LIU Wen-li
%A LIU Guang-shen
%A LIU Wei-ping
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 7
%P 1157~1163
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A1157

TY - JOUR
T1 - Effects of Hg and Cu on the activities of soil acid phosphatase
A1 - XU Dong-mei
A1 - CHEN Bo
A1 - LIU Wen-li
A1 - LIU Guang-shen
A1 - LIU Wei-ping
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 7
SP - 1157
EP - 1163
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A1157


Abstract: 
Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, soil 2 took the second place, and soil 3 was the least contaminated. Except soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Banerjee, M.R., Burton, D.L., Depoe, S., 1997. Impact of sewage sludge application on soil biological characteristics. Agriculture Ecosystems and Environment, 66(3):241-249.

[2] Brookes, P.C., 1995. The use of microbial parameters in monitoring soil pollution by heavy metals. Biology and Fertility of Soils, 19(4):269-279.

[3] Cenci, G., Morozzi, G., Caldini, G., 1985. Injury by heavy metala in escherichiacoli. Bull. Environ. Contam. Toxicol., 34(1):188-195.

[4] Chen, F., Cao, H., Pu, L.J., Peng, B.Z., 2002. Effects of Zn2+ on microbial carbon and respiration in soil. Chinese Journal of Inorganic Chemistry, 18(4):404-408 (in Chinese).

[5] Chen, H.M., Zheng, C.R., Wang, S.Q., Tu, C., 2000. Combined pollution and pollution index of heavy metals in Red Soil. Pedosphere, 10(2):117-124.

[6] Deng, S.P., Tabatabai, M.A., 1995. Cellulase activity of soils: effect of trace elements. Soil Biology & Biochemistry, 27(7):977-979.

[7] Doelman, P., Haanstra, L., 1989. Short- and long-term effects of heavy metals on phosphatase activity: an ecological dose-response model approach. Biology and Fertility of Soils, 8(3):235-241.

[8] Eivazi, F., Tabatabai, M.A., 1990. Factors affecting glucosidase and galactosidase activities in soils. Soil Biology & Biochemistry, 22(7):891-897.

[9] Frankenberger, W.T.Jr., Tabatabai, M.A., 1981. Amidase activity in soils: IV. Effects of trace elements and pesticides. Soil Science Society of America Journal, 45(6):1120-1124.

[10] Frankenberger, W.T.Jr., Tabatabai, M.A., 1991a. Factors affecting L-asparaginase activity in soils. Biology and Fertility of Soils, 11(1):1-5.

[11] Frankenberger, W.T.Jr., Tabatabai, M.A., 1991b. Factors affecting L-glutaminase activity in soils. Soil Biology & Biochemistry, 23(9):875-879.

[12] Geiger, G., Brandl, H., Furrer, G., Schulin, R., 1998a. The effect of copper on the activity of cellulase and β-glucosidase in the presence of montmorillonite or Al-montmorillonite. Soil Biology & Biochemistry, 30(12):1537-1544.

[13] Geiger, G., Livingston, M.P., Funk, F., Schulin, R., 1998b. β-glucosidase activity in the presence of copper and goethite. European Journal of Soil Science, 49(1):17-23.

[14] Giller, K.E., Witter, E., McGrath, S.P., 1998. Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils: A review. Soil Biology & Biochemistry, 30(10-11):1389-1414.

[15] Renella, G., Ortigoza, A.L.R., Landi, L., Nannipieri, P., 2003. Additive effects of copper and zinc on cadmium toxicity on phosphatase activites and ATP content of soil as estimated by the ecological dose (ED50). Soil Biology & Biochemistry, 35(9):1203-1210.

[16] He, W.X., Zhu, M.E., 1997. Study on relation between urease and fertility of soils in Shaanxi II. Soil urease kinetic characteristics. Acta Pedologica Sinica, 34(1):42-52 (in Chinese).

[17] He, W.X., Wei, G.H., Wu, Y.J., Zhu, M.E., 2001. Soil enzyme activity effected by Hg. China Environmental Sciences, 21(3):279-283 (in Chinese).

[18] He, W.X., Huang, Y.F., Zhu, M.E., Zhang, Y.P., 2002. Relationship between urease activity and fertility of soils in Shaanxi Province. Acta Pedologica Sinica, 39(3):412-420 (in Chinese).

[19] Kandeler, E., Kampichler, C., Horak, O., 1997. The influence of heavy metals on the functional diversity of soil microbial communities. Biology and Fertility of Soils, 23(3):299-306.

[20] Lin, Y.S., 1995. Study on soil buffering capacity to zinc. Acta Scientiae Circomstantian, 15(3):289-294 (in Chinese).

[21] Marzadori, C., Ciavatta, C., Montccchio, D., Gcssa, C., 1996. Effects of lead pollution on different soil enzymes. Biology and Fertility of Soils, 22(1-2):53-58.

[22] Tu, C., 1993. Study on difference in ecological and environmental effects of copper in different soils. China Environmental Sciences, 13(5):361-366 (in Chinese).

[23] Tu, C., Zheng, C.R., Chen, H.M., 2000. Effect of heavy metal pollution on potassium behavior in typic udic ferrisol. Pedosphere, 10(1):21-30.

[24] Zhu, X.C., Qing, C.L., Pi, G.J., 1996. Study on soil mercury fractions and their influencing factors. Acta Pedologica Sinica, 33(1):94-100 (in Chinese).

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