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Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.7 P.1157~1163


Effects of Hg and Cu on the activities of soil acid phosphatase

Author(s):  XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping

Affiliation(s):  Institute of Environmental Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   xudongmei@zju.edu.cn

Key Words:  Acid phosphatase (ACPase), Activity, Heavy metal, Kinetic property, Soil

XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping. Effects of Hg and Cu on the activities of soil acid phosphatase[J]. Journal of Zhejiang University Science A, 2007, 8(7): 1157~1163.

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author="XU Dong-mei, CHEN Bo, LIU Wen-li, LIU Guang-shen, LIU Wei-ping",
journal="Journal of Zhejiang University Science A",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of Hg and Cu on the activities of soil acid phosphatase
%A XU Dong-mei
%A LIU Wen-li
%A LIU Guang-shen
%A LIU Wei-ping
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 7
%P 1157~1163
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A1157

T1 - Effects of Hg and Cu on the activities of soil acid phosphatase
A1 - XU Dong-mei
A1 - CHEN Bo
A1 - LIU Wen-li
A1 - LIU Guang-shen
A1 - LIU Wei-ping
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 7
SP - 1157
EP - 1163
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A1157

Comparative study on the activity and kinectic properties of acid phosphatase (ACPase) of three soils amended with Hg and Cu at constant temperature and humidity was carried out. The results indicated that the inhibition on ACPase of the three sample soils by Hg and Cu varied with the content of soil organic matter and pH, where, soil 1 was the most seriously contaminated due to its lowest content of organic matter and the lowest pH among three samples, soil 2 took the second place, and soil 3 was the least contaminated. Except soil 3, the activity of soil ACPase tended to increase along with the contact time under the same type and the same concentration of heavy metal. In particular the Vmax values of ACPase in all three samples decreased with increasing Hg and Cu concentration, whereas the Km values were affected weakly. According to the change of Vmax and Km values, Cu and Hg had the same inhibition effect on soil ACPase. Both of them may be a type of compound of non-competitive and anti-competitive inhibition. Statistic analyses indicated that activities of soil ACPase and Vmax values could serve as bioindicator to partially denote the heavy metal Hg and Cu contamination degree.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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