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CLC number: Q813.1+1; R734.2

On-line Access: 2010-06-02

Received: 2009-11-11

Revision Accepted: 2010-03-28

Crosschecked: 2010-04-28

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Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.6 P.458-464


Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells

Author(s):  Wei Li, Jian-xiang Wu, You-ying Tu

Affiliation(s):  Department of Tea Science, Zhejiang University, Hangzhou 310029, China, Department of Tea Culture, Zhejiang Shuren University, Hangzhou 310015, China, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   youytu@zju.edu.cn

Key Words:  Theaflavin-3-3′, -digallate (TF3), (−, )-epigallocatechin-3-gallate (EGCG), Ascorbic acid (AA), Synergism, SPC-A-1 cells, Cell cycle

Wei Li, Jian-xiang Wu, You-ying Tu. Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells[J]. Journal of Zhejiang University Science B, 2010, 11(6): 458-464.

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author="Wei Li, Jian-xiang Wu, You-ying Tu",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells
%A Wei Li
%A Jian-xiang Wu
%A You-ying Tu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 6
%P 458-464
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900355

T1 - Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells
A1 - Wei Li
A1 - Jian-xiang Wu
A1 - You-ying Tu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 6
SP - 458
EP - 464
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900355

Tea polyphenols have been shown to have anticancer activity in many studies. In the present study, we investigated effects of theaflavin-3-3′;-digallate (TF3), one of the major theaflavin monomers in black tea, in combination with ascorbic acid (AA), a reducing agent, and (−;)-epigallocatechin-3-gallate (EGCG), the main polyphenol presented in green tea, in combination with AA on cellular viability and cell cycles of the human lung adenocarcinoma SPC-A-1 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the 50% inhibition concentrations (IC50) of TF3, EGCG, and AA on SPC-A-1 cells were 4.78, 4.90, and 30.62 μmol/L, respectively. The inhibitory rates of TF3 combined with AA (TF3+AA) and EGCG combined with AA (EGCG+AA) at a molar ratio of 1:6 on SPC-A-1 cells were 54.4% and 45.5%, respectively. Flow cytometry analysis showed that TF3+AA and EGCG+AA obviously increased the cell population in the G0/G1 phase of the SPC-A-1 cell cycle from 53.9% to 62.8% and 60.0%, respectively. TF3-treated cells exhibited 65.3% of the G0/G1 phase at the concentration of its IC50. Therefore, TF3+AA and EGCG+AA had synergistic inhibition effects on the proliferation of SPC-A-1 cells, and significantly held SPC-A-1 cells in G0/G1 phase. The results suggest that the combination of TF3 with AA or EGCG with AA enhances their anticancer activity.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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