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Abstract: Tea polyphenols have been shown to have anticancer activity in many studies. In the present study, we investigated effects of theaflavin-3-3′;-digallate (TF₃), one of the major theaflavin monomers in black tea, in combination with ascorbic acid (AA), a reducing agent, and (−;)-epigallocatechin-3-gallate (EGCG), the main polyphenol presented in green tea, in combination with AA on cellular viability and cell cycles of the human lung adenocarcinoma SPC-A-1 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the 50% inhibition concentrations (IC₅₀) of TF₃, EGCG, and AA on SPC-A-1 cells were 4.78, 4.90, and 30.62 μmol/L, respectively. The inhibitory rates of TF₃ combined with AA (TF₃+AA) and EGCG combined with AA (EGCG+AA) at a molar ratio of 1:6 on SPC-A-1 cells were 54.4% and 45.5%, respectively. Flow cytometry analysis showed that TF₃+AA and EGCG+AA obviously increased the cell population in the G₀/G₁ phase of the SPC-A-1 cell cycle from 53.9% to 62.8% and 60.0%, respectively. TF₃-treated cells exhibited 65.3% of the G₀/G₁ phase at the concentration of its IC₅₀. Therefore, TF₃+AA and EGCG+AA had synergistic inhibition effects on the proliferation of SPC-A-1 cells, and significantly held SPC-A-1 cells in G₀/G₁ phase. The results suggest that the combination of TF₃ with AA or EGCG with AA enhances their anticancer activity.

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