Full Text:   <1198>

CLC number: Q813.1+1; R734.2

On-line Access: 2010-06-02

Received: 2009-11-11

Revision Accepted: 2010-03-28

Crosschecked: 2010-04-28

Cited: 16

Clicked: 4351

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2010 Vol.11 No.6 P.458-464

http://doi.org/10.1631/jzus.B0900355


Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells


Author(s):  Wei Li, Jian-xiang Wu, You-ying Tu

Affiliation(s):  Department of Tea Science, Zhejiang University, Hangzhou 310029, China, Department of Tea Culture, Zhejiang Shuren University, Hangzhou 310015, China, Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   youytu@zju.edu.cn

Key Words:  Theaflavin-3-3′, -digallate (TF3), (−, )-epigallocatechin-3-gallate (EGCG), Ascorbic acid (AA), Synergism, SPC-A-1 cells, Cell cycle


Wei Li, Jian-xiang Wu, You-ying Tu. Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells[J]. Journal of Zhejiang University Science B, 2010, 11(6): 458-464.

@article{title="Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells",
author="Wei Li, Jian-xiang Wu, You-ying Tu",
journal="Journal of Zhejiang University Science B",
volume="11",
number="6",
pages="458-464",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0900355"
}

%0 Journal Article
%T Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells
%A Wei Li
%A Jian-xiang Wu
%A You-ying Tu
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 6
%P 458-464
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0900355

TY - JOUR
T1 - Synergistic effects of tea polyphenols and ascorbic acid on human lung adenocarcinoma SPC-A-1 cells
A1 - Wei Li
A1 - Jian-xiang Wu
A1 - You-ying Tu
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 6
SP - 458
EP - 464
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0900355


Abstract: 
Tea polyphenols have been shown to have anticancer activity in many studies. In the present study, we investigated effects of theaflavin-3-3′;-digallate (TF3), one of the major theaflavin monomers in black tea, in combination with ascorbic acid (AA), a reducing agent, and (−;)-epigallocatechin-3-gallate (EGCG), the main polyphenol presented in green tea, in combination with AA on cellular viability and cell cycles of the human lung adenocarcinoma SPC-A-1 cells. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay showed that the 50% inhibition concentrations (IC50) of TF3, EGCG, and AA on SPC-A-1 cells were 4.78, 4.90, and 30.62 μmol/L, respectively. The inhibitory rates of TF3 combined with AA (TF3+AA) and EGCG combined with AA (EGCG+AA) at a molar ratio of 1:6 on SPC-A-1 cells were 54.4% and 45.5%, respectively. Flow cytometry analysis showed that TF3+AA and EGCG+AA obviously increased the cell population in the G0/G1 phase of the SPC-A-1 cell cycle from 53.9% to 62.8% and 60.0%, respectively. TF3-treated cells exhibited 65.3% of the G0/G1 phase at the concentration of its IC50. Therefore, TF3+AA and EGCG+AA had synergistic inhibition effects on the proliferation of SPC-A-1 cells, and significantly held SPC-A-1 cells in G0/G1 phase. The results suggest that the combination of TF3 with AA or EGCG with AA enhances their anticancer activity.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Agarwal, R., Mukhtar, H., 1996. Cancer chemoprevention by polyphenols in green tea and artichoke. Advances in Experimental Medicine and Biology, 401:35-50.

[2]Chou, T.C., Talalay, P., 1984. Quantitative analysis of dose-effect relationships: the combined effects of multiple drugs or enzyme inhibitors. Advances in Enzyme Regulation, 22:27-55.

[3]Chung, J.Y., Huang, C.S., Meng, X.F., Yang, C.S., 1999. Inhibition of activator protein 1 activity and cell growth by purified green tea and black tea polyphenols in H-ras-transformed cells: structure-activity relationship and mechanisms involved. Cancer Research, 59(18):4610-4617.

[4]Conney, A.H., Lu, Y.P., Lou, Y.R., Xie, J.P., Huang, M.T., 1999. Inhibitory effect of green and black tea on tumor growth. Proceedings of the Society for Experimental Biology and Medicine, 220(4):229-233.

[5]Denizot, F., Lang, R., 1986. Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods, 89(2):271-277.

[6]Hatano, T., Kusuda, M., Hori, M., Shiota, S., Tsuchiya, T., Yoshida, T., 2003. Theasinensin A, a tea polyphenol formed from (−)-epigallocatechin gallate, suppresses antibiotic resistance of methicillin-resistant Staphylococcus aureus. Planta Medica, 69(11):984-989.

[7]Hong, J.G., Smith, T.J., Ho, C.T., August, D.A., Yang, C.S., 2001. Effects of purified green and black tea polyphenols on cyclooxygenase and lipoxygenase-dependent metabolism of arachidonic acid in human colon mucosa and colon tumor tissues. Biochemical Pharmacology, 62(9):1175-1183.

[8]Kaack, K., Austed, T., 1998. Interaction of vitamin C and flavonoids in elderberry (Sambucus nigra L.) during juice processing. Plant Foods for Human Nutrition, 52(3):187-198.

[9]Kim, K.N., Pie, J.E., Park, J.H., Park, Y.H., Kim, H.W., Kim, M.K., 2006. Retinoic acid and ascorbic acid act synergistically in inhibition human breast cancer cell proliferation. The Journal of Nutritional Biochemistry, 17(7):454-462.

[10]Leung, L.K., Su, Y., Chen, R., Zhang, Z., Huang, Y., Chen, Z.Y., 2001. Theaflavins in black tea and catechins in green tea are equally effective antioxidants. Journal of Nutrition, 131(9):2248-2251.

[11]Lu, J.B., Ho, C.T., Ghai, G., Chen, K.Y., 2000. Differential effects of theaflavin monogallates on cell growth, apoptosis, and Cox-2 gene expression in cancerous versus normal cells. Cancer Research, 60(22):6465-6471.

[12]Lung, H.L., Ip, W.K., Chen, Z.Y., Mak, N.K., Leung, K.N., 2004. Comparative study of the growth-inhibitory and apoptosis-inducing activities of black tea theaflavins and green tea catechin on murine myeloid leukemia cells. International Journal of Molecular Medicine, 13(3):465-471.

[13]Mittal, A., Pate, M.S., Wylie, R.C., Tollefsbol, T.O., Katiyar, S.K., 2004. EGCG down-regulates telomerase in human breast carcinoma MCF-7 cells, leading to suppression of cell viability and induction of apoptosis. International Journal of Oncology, 24(3):703-710.

[14]Moon, Y., Lee, M., Yang, H., 2007. Involvement of early growth response gene 1 in the modulation of microsomal prostaglandin E synthase 1 by epigallocatechin gallate in A549 human pulmonary epithelial cells. Biochemical Pharmacology, 73(1):125-135.

[15]Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65(1-2):55-63.

[16]Pan, M.H., Lin-Shiau, S.Y., Ho, C.T., Lin, J.H., Lin, J.K., 2000. Suppression of lipopolysaccharide-induced nuclear factor-kappa B activity by theaflavin-3,3'-digallate from black tea and other polyphenols through down-regulation of I kappa B kinase activity in macrophages. Biochemical Pharmacology, 59(4):357-367.

[17]Roomi, M.W., Ivanov, V., Kalinovsky, T., Niedzwiecki, A., Rath, M., 2006. Inhibition of matrix metalloproteinase-2 secretion and invasion by human ovarian cancer cell line SK-OV-3 with lysine, proline, arginine, ascorbic acid and green tea extract. Journal of Obstetrics and Gynaecology Research, 32(2):148-154.

[18]Saeki, K., Sano, M., Miyase, T., Nakamura, Y., Hara, Y., Aoyagi, Y., Isemura, M., 1999. Apoptosis-inducing activity of polyphenol compounds derived from tea catechins in human histiolytic lymphoma U937 cells. Bioscience Biotechnology and Biochemistry, 63(3):585-587.

[19]Tu, Y.Y., Xia, H.L., 2004. Immobilized Polyphenol Oxidase Catalyze Purified Tea Polyphenols into High Quality Theaflavins. China Patent 02136982.8 ZL.

[20]Tu, Y.Y., Tang, A.B., Watanabe, N., 2004. The theaflavin monomers inhibit the cancer cells growth in vitro. Acta Biochimica et Biophysica Sinica, 36(7):508-512.

[21]Weisburg, J.H., Weissman, D.B., Sedaghat, T., Babich, H., 2004. In vitro cytotoxicity of epigallocatechin gallate and tea extracts to cancerous and normal cells from the human oral cavity. Basic & Clinical Pharmacology & Toxicology, 95(4):191-200.

[22]Yang, C.S., Lambert, J.D., Ju, J., Lu, G., Sang, S., 2007. Tea and cancer prevention: molecular mechanisms and human relevance. Toxicology and Applied Pharmacology, 224(3):265-273.

[23]Yang, C.S., Wang, X., Lu, G., Picinich, S.C., 2009. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nature Reviews Cancer, 9(6):429-439.

[24]Yang, G.Y., Liao, J., Kim, K., Yurkow, E.J., Yang, C.S., 1998. Inhibition of growth and induction of apoptosis in human cancer cell lines by tea polyphenols. Carcinogenesis, 19(4):611-616.

[25]Yang, Z., Jie, G., Dong, F., Xu, Y., Watanabe, N., Tu, Y., 2008. Radical-scavenging abilities and antioxidant properties of theaflavins and their gallate esters in H2O2-mediated oxidative damage system in the HPF-1 cells. Toxicology in Vitro, 22(5):1250-1256.

[26]Yang, Z.Y., Tu, Y.Y., Xia, H.L., Jie, G.L., Chen, X.M., He, P.M., 2007. Suppression of free-radicals and protection against H2O2-induced oxidative damage in HPF-1 cell by oxidized phenolic compounds present in black tea. Food Chemistry, 105(4):1349-1356.

[27]Zheng, Q.S., Zhang, Y.T., Zheng, R.L., 2002. Ascorbic acid induces redifferentiation and growth inhibition in human hepatoma cells by increasing endogenous hydrogen peroxide. Pharimazie, 57(11):753-757.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE