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Journal of Zhejiang University SCIENCE B 2005 Vol.6 No.7 P.693~698

http://doi.org/10.1631/jzus.2005.B0693


Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma


Author(s):  PAN Jian-wei, ZHAN Ren-ya, TONG Ying, ZHOU Yong-qing, ZHANG Ming

Affiliation(s):  Department of Neurosurgery, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China; more

Corresponding email(s):   swordman_pan@hotmail.com

Key Words:  Endothelial nitric oxide synthase, Vascular endothelial growth factor, Density of microvessels, Primary astrocytoma


PAN Jian-wei, ZHAN Ren-ya, TONG Ying, ZHOU Yong-qing, ZHANG Ming. Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma[J]. Journal of Zhejiang University Science B, 2005, 6(7): 693~698.

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author="PAN Jian-wei, ZHAN Ren-ya, TONG Ying, ZHOU Yong-qing, ZHANG Ming",
journal="Journal of Zhejiang University Science B",
volume="6",
number="7",
pages="693~698",
year="2005",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2005.B0693"
}

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%T Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma
%A PAN Jian-wei
%A ZHAN Ren-ya
%A TONG Ying
%A ZHOU Yong-qing
%A ZHANG Ming
%J Journal of Zhejiang University SCIENCE B
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%N 7
%P 693~698
%@ 1673-1581
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2005.B0693

TY - JOUR
T1 - Expression of endothelial nitric oxide synthase and vascular endothelial growth factor in association with neovascularization in human primary astrocytoma
A1 - PAN Jian-wei
A1 - ZHAN Ren-ya
A1 - TONG Ying
A1 - ZHOU Yong-qing
A1 - ZHANG Ming
J0 - Journal of Zhejiang University Science B
VL - 6
IS - 7
SP - 693
EP - 698
%@ 1673-1581
Y1 - 2005
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2005.B0693


Abstract: 
Objective: To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) and angiogenesis in primary astrocytoma. Methods: Thirty-seven primary astrocytomas and 4 astrocytic hyperplasia samples were collected and divided into three groups according to histological grade. The expression of eNOS, VEGF and factor VIII related antigen (FVIIIRAg) were assayed by immunohistochemistry. Microvascular density was assessed by FVIIIRAg immunoreactivity. The intensity of immunoreactivity was graded according to the percentage of positive tumor cells. Results: No eNOS and VEGF were expressed in the astrocytes and vascular endothelium in astrocytic hyperplasia. The expression of eNOS or VEGF was light in low-grade astrocytoma and strong in glioblastoma. eNOS expression in astrocytoma was very positively correlated with VEGF. eNOS and VEGF expression in anaplastic astrocytoma was median in contrast to the low grade astrocytoma and glioblastoma. Lower microvascular density was found in low grade astrocytoma than that in higher grade malignant ones. The expressions of eNOS and VEGF were correlated with microvascular density and tumor malignancy. Conclusion: This finding suggests that eNOS and VEGF may have cooperative effect in tumor angiogenesis and play an important role in the pathogenesis of primary astrocytoma.

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Reference

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