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CLC number: R587.1

On-line Access: 2015-11-04

Received: 2015-04-02

Revision Accepted: 2015-07-22

Crosschecked: 2015-10-09

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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.11 P.948-956

http://doi.org/10.1631/jzus.B1500076


Impact of type 2 diabetes on the plasma levels of vascular endothelial growth factor and its soluble receptors type 1 and type 2 in patients with peripheral arterial disease


Author(s):  Radosław Wieczór, Grażyna Gadomska, Barbara Ruszkowska-Ciastek, Katarzyna Stankowska, Jacek Budzyński, Jacek Fabisiak, Karol Suppan, Grzegorz Pulkowski, Danuta Rość

Affiliation(s):  Department of Pathophysiology, Faculty of Pharmacy, Nicolaus Copernicus University in Toruń, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Bydgoszcz PL 85-094, Poland; more

Corresponding email(s):   wieczorcmumk@tlen.pl

Key Words:  Angiogenesis, Peripheral arterial disease, Soluble receptors, Type 2 diabetes mellitus, Vascular endothelial growth factor


Radosław Wieczór, Grażyna Gadomska, Barbara Ruszkowska-Ciastek, Katarzyna Stankowska, Jacek Budzyński, Jacek Fabisiak, Karol Suppan, Grzegorz Pulkowski, Danuta Rość. Impact of type 2 diabetes on the plasma levels of vascular endothelial growth factor and its soluble receptors type 1 and type 2 in patients with peripheral arterial disease[J]. Journal of Zhejiang University Science B, 2015, 16(11): 948-956.

@article{title="Impact of type 2 diabetes on the plasma levels of vascular endothelial growth factor and its soluble receptors type 1 and type 2 in patients with peripheral arterial disease",
author="Radosław Wieczór, Grażyna Gadomska, Barbara Ruszkowska-Ciastek, Katarzyna Stankowska, Jacek Budzyński, Jacek Fabisiak, Karol Suppan, Grzegorz Pulkowski, Danuta Rość",
journal="Journal of Zhejiang University Science B",
volume="16",
number="11",
pages="948-956",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1500076"
}

%0 Journal Article
%T Impact of type 2 diabetes on the plasma levels of vascular endothelial growth factor and its soluble receptors type 1 and type 2 in patients with peripheral arterial disease
%A Radosław Wieczór
%A Grażyna Gadomska
%A Barbara Ruszkowska-Ciastek
%A Katarzyna Stankowska
%A Jacek Budzyński
%A Jacek Fabisiak
%A Karol Suppan
%A Grzegorz Pulkowski
%A Danuta Rość
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 11
%P 948-956
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1500076

TY - JOUR
T1 - Impact of type 2 diabetes on the plasma levels of vascular endothelial growth factor and its soluble receptors type 1 and type 2 in patients with peripheral arterial disease
A1 - Radosław Wieczór
A1 - Grażyna Gadomska
A1 - Barbara Ruszkowska-Ciastek
A1 - Katarzyna Stankowska
A1 - Jacek Budzyński
A1 - Jacek Fabisiak
A1 - Karol Suppan
A1 - Grzegorz Pulkowski
A1 - Danuta Rość
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 11
SP - 948
EP - 956
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1500076


Abstract: 
Objective: Type 2 diabetes coexistent with lower extremity artery disease (peripheral arterial disease (PAD)) can be observed in numerous patients. The mechanism compensating for ischemia and contributing to healing is angiogenesis—the process of forming new blood vessels. The purpose of this study was to assess the likely impact of type 2 diabetes on the plasma levels of proangiogenic factor (vascular endothelial growth factor A (VEGF-A)) and angiogenesis inhibitors (soluble VEGF receptors type 1 and type 2 (sVEGFR-1 and sVEGFR-2)) in patients with PAD. Methods: Among 46 patients with PAD under pharmacological therapy (non-invasive), we identified, based on medical history, a subgroup with coexistent type 2 diabetes (PAD-DM2+, n=15) and without diabetes (PAD-DM2−, n=31). The control group consisted of 30 healthy subjects. Plasma levels of VEGF-A, sVEGFR-1, and sVEGFR-2 were measured using the enzyme-linked immunosorbent assay (ELISA) method. Results: The subgroups of PAD-DM2+ and PAD-DM2− revealed significantly higher concentrations of VEGF-A (P=0.000 007 and P=0.000 000 1, respectively) and significantly lower sVEGFR-2 levels (P=0.02 and P=0.000 01, respectively), when compared with the control group. Patients with PAD and coexistent diabetes tended to have a lower level of VEGF-A and higher levels of sVEGFR-1 and sVEGFR-2 comparable with non-diabetic patients. Conclusions: The coexistence of type 2 diabetes and PAD is demonstrated by a tendency to a lower plasma level of proangiogenic factor (VEGF-A) and higher levels of angiogenesis inhibitors (sVEGFR-1 and sVEGFR-2) at the same time. Regardless of the coexistence of type 2 diabetes, hypoxia appears to be a crucial factor stimulating the processes of angiogenesis in PAD patients comparable with healthy individuals, whereas hyperglycemia may have a negative impact on angiogenesis in lower limbs.

2型糖尿病对外周动脉疾病患者血浆内的血管内皮生长因子及其可溶性受体浓度的影响

目的:研究2型糖尿病对外周动脉疾病患者血浆内的血管内皮生长因子(VEGF-A)及其水溶性受体(sVEGFR-1和sVEGFR-2)浓度的影响。
创新点:首次研究了2型糖尿病对外周动脉疾病患者血浆内sVEGFR-1和sVEGFR-2浓度的影响。
方法:选取46个外周动脉疾病患者,根据有无2型糖尿病分为糖尿病组(15例)和无糖尿病组(31例),另选30个健康志愿者为正常对照组。采用酶联免疫吸附法(ELISA)检测他们血浆中VEGF-A及sVEGFR-1和sVEGFR-2的浓度,然后通过对比各组浓度研究2型糖尿病的影响。
结论:与正常对照组相比,外周动脉疾病患者具有较高的VEGF-A浓度(2型糖尿病组P=0.000 007,非糖尿病组P=0.000 000 1)以及较低的sVEGFR-2浓度(2型糖尿病组P=0.02,非糖尿病组P=0.000 01)。同时,2型糖尿病组比非糖尿病组具有较低的VEGF-A浓度及较高的sVEGFR-1和sVEGFR-2浓度。研究结果表明:无论2型糖尿病是否共存,缺氧是导致血管生成的一个关键的刺激因素;同时,高血糖状态对下肢的血管生成有抑制作用。

关键词:血管生成;外周动脉疾病;可溶性受体;2型糖尿病;血管内皮生长因子

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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