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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.6 P.497~502

http://doi.org/10.1631/jzus.2006.B0497


Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method


Author(s):  LIAO Fei, ZHAO Yun-sheng, ZHAO Li-na, TAO Jia, ZHU Xiao-yun, LIU Lan

Affiliation(s):  Laboratory of Bioinformatics and Molecular Engineering, Chongqing University of Medical Sciences, Chongqing 400016, China; more

Corresponding email(s):   aofeish@vip.sina.com, liaofeish@yahoo.com

Key Words:  Background absorbance, Bland-Altman analysis, Kinetic uricase method, Reaction curve fitting, Serum uric acid assay


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LIAO Fei, ZHAO Yun-sheng, ZHAO Li-na, TAO Jia, ZHU Xiao-yun, LIU Lan. Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method[J]. Journal of Zhejiang University Science B, 2006, 7(6): 497~502.

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author="LIAO Fei, ZHAO Yun-sheng, ZHAO Li-na, TAO Jia, ZHU Xiao-yun, LIU Lan",
journal="Journal of Zhejiang University Science B",
volume="7",
number="6",
pages="497~502",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0497"
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%0 Journal Article
%T Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method
%A LIAO Fei
%A ZHAO Yun-sheng
%A ZHAO Li-na
%A TAO Jia
%A ZHU Xiao-yun
%A LIU Lan
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 6
%P 497~502
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0497

TY - JOUR
T1 - Evaluation of a kinetic uricase method for serum uric acid assay by predicting background absorbance of uricase reaction solution with an integrated method
A1 - LIAO Fei
A1 - ZHAO Yun-sheng
A1 - ZHAO Li-na
A1 - TAO Jia
A1 - ZHU Xiao-yun
A1 - LIU Lan
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 6
SP - 497
EP - 502
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0497


Abstract: 
A patented kinetic uricase method was evaluated for serum uric acid assay. Initial absorbance of the reaction mixture before uricase action (A0) was obtained by correcting the absorbance at 293 nm measured before the addition of uricase solution, and background absorbance (Ab) was predicted by an integrated method. Uric acid concentration in reaction solution was calculated from ∆A, the difference between A0 and Ab, using the absorptivity preset for uric acid. This kinetic uricase method exhibited CV<4.3% and recovery of 100%. Lipids, bilirubin, hemoglobin, ascorbic acid, reduced glutathione and xanthine <0.32 mmol/L in serum had no significant effects. ∆A linearly responded to 1.2 to 37.5 μmol/L uric acid in reaction solution containing 15 μl serum. The slope of linear response was consistent with the absorptivity preset for uric acid while the intercept was consistent with that for serum alone. Uric acid concentrations in clinic sera by different uricase methods positively correlated to each other. By bland-Altman analysis, this kinetic uricase method accorded with that by quantifying the total change of UV absorbance on the completion of uricase reaction. These results demonstrated that this kinetic uricase method is reliable for serum uric acid assay with enhanced resistance to both xanthine and other common errors, wider range of linear response and much lower cost.

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Reference

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