Full Text:   <2688>

CLC number: S85

On-line Access: 

Received: 2006-07-10

Revision Accepted: 2006-09-19

Crosschecked: 0000-00-00

Cited: 12

Clicked: 5388

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.922-928


Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk

Author(s):  LI Xiao-liang, SHUAI Jiang-bing, FANG Wei-huan

Affiliation(s):  Institute of Preventive Veterinary Medicine and Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   whfang@zju.edu.cn

Key Words:  Aeromonas hydrophila, Immunoglobulins, Hen egg yolk, Polyploid gibel carps, Carassius auratus Gibelio

LI Xiao-liang, SHUAI Jiang-bing, FANG Wei-huan. Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk[J]. Journal of Zhejiang University Science B, 2006, 7(11): 922-928.

@article{title="Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk",
author="LI Xiao-liang, SHUAI Jiang-bing, FANG Wei-huan",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk
%A LI Xiao-liang
%A SHUAI Jiang-bing
%A FANG Wei-huan
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 922-928
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0922

T1 - Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk
A1 - LI Xiao-liang
A1 - SHUAI Jiang-bing
A1 - FANG Wei-huan
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 922
EP - 928
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0922

Specific immunoglobulin (IgY) from egg yolk against Aeromonas hydrophila was produced by immunization of White Leghorn hens with formalin-killed whole cells of A. hydrophila. ELISA test using A. hydrophila as the coating antigen revealed that the specific antibody titer started to increase in the egg yolk at the 13th day post-immunization (P/N=2.18), reached the peak at the 56th day (P/N=13.82), and remained at high level until day 133 (P/N=7.03). The antibody was purified by saturated ammonium sulphate with a recovery rate of 63.5%. The specific IgY inhibited the growth of A. hydrophila at a concentration of 1.0 mg/ml during the 18 h incubation. Pre-treatment of polyploid gibel carps Carassius auratus Gibelio with specific IgY had a protection rate of 60% (6/10) against challenge with A. hydrophila, while none of the fishes in the control groups receiving sterile phosphate buffered saline (PBS) or non-specific IgY survived the challenge. Treatment of fishes with the specific IgY 4 h after the challenge also had lower mortality (70%, 7/10), a 30% reduction against the control PBS or non-specific IgY groups (10/10). These results indicate that specific IgY antibodies could be obtained easily from hens immunized with an inactivated A. hydrophila and could provide a novel alternative approach to control of diseases in fishes caused by this organism.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


[1] Akita, E.M., Nakai, S., 1993. Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with an enterotoxigenic E. coli strain. J. Immunol. Methods, 160(2):207-214.

[2] Asha, A., Nayak, D.K., Shankar, K.M., Mohan, C.V., 2004. Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish. Fish Shellfish Immunol., 16(3):429-436.

[3] Austin, B., Austin, D.A., Dalsgaard, I., Gudmundsdottir, B.K., Hoie, S., Thornton, J.M., Larsen, J.L., O'Hici, B., Powell, R., 1998. Characterization of atypical Aeromonas salmonicida by different methods. Syst. Appl. Microbiol., 21(1):50-64.

[4] Camenisch, G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann, P., Wenger, R.H., Gassmann, M., 1999. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1alpha. FASEB J., 13(1):81-88.

[5] Carlander, D., Kollberg, H., Wejaker, P.E., Larsson, A., 2000. Peroral immunotherapy with yolk antibodies for the prevention and treatment of enteric infections. Immunol. Res., 21(1):1-6.

[6] Chen, H.Q., Lu, C.P., 1991. Study on pathogen of bacterial hemorrhagic septicemia of rice eel. Chinese Journal of Zoonoses, 7(4):21-23 (in Chinese).

[7] Chowdhury, M.A., Yamanaka, H., Miyoshi, S., Shinoda, S., 1990. Ecology of mesophilic Aeromonas spp. in aquatic environments of a temperate region and relationship with some biotic and abiotic environmental parameters. Zentralbl. Hyg. Umweltmed., 190(4):344-356.

[8] Ebina, T., 1996. Prophylaxis of rotavirus gastroenteritis using immunoglobulin. Arch. Virol. Suppl., 12:217-223.

[9] Elwitigala, J.P., Higgs, D.S., Namnyak, S., White, J.W., Yaneza, A., 2005. Septic arthritis due to Aeromonas hydrophila: case report and review of the literature. Int. J. Clin. Pract., 59(s147):121-124.

[10] Gonzalez, C.J., Santos, J.A., Garcia-Lopez, M.L., Gonzalez, N., Otero, A., 2001. Mesophilic aeromonads in wild and aquacultured freshwater fish. J. Food Prot., 64(5):687-691.

[11] Hatta, H., Tsuda, K., Ozeki, M., Kim, M., Yamamoto, T., Otake, S., Hirasawa, M., Katz, J., Childers, N.K., Michalek, S.M., 1997. Passive immunization against dental plaque formation in humans: effect of a mouth rinse containing egg yolk antibodies (IgY) specific to Streptococcus mutans. Caries Res., 31(4):268-274.

[12] Hennig-Pauka, I., Stelljes, I., Waldmann, K.H., 2003. Studies on the effect of specific egg antibodies against Escherichia coli infections in piglets. Dtsch. Tierarztl. Wochenschr., 110(2):49-54.

[13] Ikemori, Y., Kuroki, M., Peralta, R.C., Yokoyama, H., Kodama, Y., 1992. Protection of neonatal calves against fatal enteric colibacillosis by administration of egg yolk powder from hens immunized with K99-piliated enterotoxigenic Escherichia coli. Am. J. Vet. Res., 53(11):2005-2008.

[14] Janda, J.M., Abbott, S.L., 1998. Evolving concepts regarding the genus Aeromonas: an expanding Panorama of species, disease presentations, and unanswered questions. Clin. Infect. Dis., 27(2):332-344.

[15] Kampfer, P., Christmann, C., Swings, J., Huys, G., 1999. In vitro susceptibilities of Aeromonas genomic species to 69 antimicrobial agents. Syst. Appl. Microbiol., 22(4):662-669.

[16] Kariyawasam, S., Wilkie, B.N., Gyles, C.L., 2004. Resistance of broiler chickens to Escherichia coli respiratory tract infection induced by passively transferred egg-yolk antibodies. Vet. Microbiol., 98(3-4):273-284.

[17] Korbsrisate, S., Dumnin, S., Chawengkirttikul, R., Gherunpong, V., Eampokalap, B., Gongviseisoog, C., Janyapoon, K., Lertpocasombat, K., Shimada, T., 2002. Distribution of Aeromonas hydrophila serogroups in different clinical samples and the development of polyclonal antibodies for rapid identification of the genus Aeromonas by direct agglutination. Microbiol. Immunol., 46(12):875-879.

[18] Krüger, C., Pearson, S.K., Kodama, Y., Vacca Smith, A., Bowen, W.H., Hammarstrom, L., 2004. The effects of egg-derived antibodies to glucosyltransferases on dental caries in rats. Caries Res., 38(1):9-14.

[19] Kuroki, M., Ohta, M., Ikemori, Y., Icatlo, F.C.Jr, Kobayashi, C., Yokoyama, H., Kodama, Y., 1997. Field evaluation of chicken egg yolk immunoglobulins specific for bovine rotavirus in neonatal calves. Arch. Virol., 142(4):843-851.

[20] Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259):680-685.

[21] Larsson, A., Carlander, D., 2003. Oral immunotherapy with yolk antibodies to prevent infections in humans and animals. Ups. J. Med. Sci., 108(2):129-140.

[22] Lee, S.B., Mine, Y., Stevenson, R.M., 2000. Effects of hen egg yolk immunoglobulin in passive protection of rainbow trout against Yersinia ruckeri. J. Agric. Food Chem., 48(1):110-115.

[23] Lee, E.N., Sunwoo, H.H., Menninen, K., Sim, J.S., 2002. In vitro studies of chicken egg yolk antibody (IgY) against Salmonella enteritidis and Salmonella typhimurium. Poult. Sci., 81(5):632-641.

[24] Marquardt, R.R., Jin, L.Z., Kim, J.W., Fang, L., Frohlich, A.A., Baidoo, S.K., 1999. Passive protective effect of egg-yolk antibodies against enterotoxigenic Escherichia coli K88+ infection in neonatal and early-weaned piglets. FEMS Immunol. Med. Microbiol., 23(4):283-288.

[25] Mine, Y., Kovacs-Nolan, J., 2002. Chicken egg yolk antibodies as therapeutics in enteric infectious disease. J. Med. Food, 5(3):159-169.

[26] Patterson, R., Youngner, J.S., Weigle, W.O., Dixon, F.J., 1962. Antibody production and transfer to egg yolk in chickens. J. Immunol., 89:272-278.

[27] Rosenberger, J.K., Fries, P.A., Cloud, S.S., 1985. In vitro and in vivo characterization of avian Escherichia coli. III. Immunization. Avian Dis., 29(4):1108-1117.

[28] Sarker, S.A., Casswall, T.H., Juneja, L.R., Hoq, E., Hossain, I., Fuchs, G.J., Hammarstrom, L., 2001. Randomized, placebo-controlled, clinical trial of hyperimmunized chicken egg yolk immunoglobulin in children with rotavirus diarrhea. J. Pediatr. Gastroenterol. Nutr., 32(1):19-25.

[29] Shin, J.H., Yang, M., Nam, S.W., Kim, J.T., Myung, N.H., Bang, W.G., Roe, I.H., 2002. Use of egg yolk-derived immunoglobulin as an alternative to antibiotic treatment for control of Helicobacter pylori infection. Clin. Diagn. Lab. Immunol., 9(5):1061-1066.

[30] Smith, D.J., King, W.F., Godiska, R., 2001. Passive transfer of immunoglobulin Y antibody to Streptococcus mutans glucan binding protein B can confer protection against experimental dental caries. Infect. Immun., 69(5):3135-3142.

[31] Sugita-Konishi, Y., Shibata, K., Yun, S.S., Hara-Kudo, Y., Yamaguchi, K., Kumagai, S., 1996. Immune functions of immunoglobulin Y isolated from egg yolk of hens immunized with various infectious bacteria. Biosci. Biotechnol. Biochem., 60(5):886-888.

[32] Tini, M., Jewell, U.R., Camenisch, G., Chilov, D., Gassmann, M., 2002. Generation and application of chicken egg-yolk antibodies. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 131(3):569-574.

[33] van Nguyen, S., Umeda, K., Yokoyama, H., Tohya, Y., Kodama, Y., 2006. Passive protection of dogs against clinical disease due to Canine parvovirus-2 by specific antibody from chicken egg yolk. Can. J. Vet. Res., 70(1):62-64.

[34] Yokoyama, H., Peralta, R.C., Diaz, R., Sendo, S., Ikemori, Y., Kodama, Y., 1992. Passive protective effect of chicken egg yolk immunoglobulins against experimental enterotoxigenic Escherichia coli infection in neonatal piglets. Infect. Immun., 60(3):998-1007.

Open peer comments: Debate/Discuss/Question/Opinion


Please provide your name, email address and a comment

Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE