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Journal of Zhejiang University SCIENCE B 2006 Vol.7 No.11 P.929~932

10.1631/jzus.2006.B0929


IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1


Author(s):  RUAN Wen-jing, LIN Jie, XU En-ping, XU Fang-ying, MA Yu, DENG Hong, HUANG Qiong, LV Bing-jian, HU Hu, CUI Jing, DI Mei-juan, DONG Jian-kang, LAI Mao-de

Affiliation(s):  Department of Pathology, School of Medicine, Zhejiang University, Hangzhou 310031, China; more

Corresponding email(s):   lmp@zju.edu.cn

Key Words:  IGFBP7 (Insulin-like growth factor binding-protein-7), Colorectal cancer, Tumor suppressor protein, Methylation


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RUAN Wen-jing, LIN Jie, XU En-ping, XU Fang-ying, MA Yu, DENG Hong, HUANG Qiong, LV Bing-jian, HU Hu, CUI Jing, DI Mei-juan, DONG Jian-kang, LAI Mao-de. IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1[J]. Journal of Zhejiang University Science B, 2006, 7(11): 929~932.

@article{title="IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1",
author="RUAN Wen-jing, LIN Jie, XU En-ping, XU Fang-ying, MA Yu, DENG Hong, HUANG Qiong, LV Bing-jian, HU Hu, CUI Jing, DI Mei-juan, DONG Jian-kang, LAI Mao-de",
journal="Journal of Zhejiang University Science B",
volume="7",
number="11",
pages="929~932",
year="2006",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2006.B0929"
}

%0 Journal Article
%T IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1
%A RUAN Wen-jing
%A LIN Jie
%A XU En-ping
%A XU Fang-ying
%A MA Yu
%A DENG Hong
%A HUANG Qiong
%A LV Bing-jian
%A HU Hu
%A CUI Jing
%A DI Mei-juan
%A DONG Jian-kang
%A LAI Mao-de
%J Journal of Zhejiang University SCIENCE B
%V 7
%N 11
%P 929~932
%@ 1673-1581
%D 2006
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2006.B0929

TY - JOUR
T1 - IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis with its expression associated with DNA hypomethylation of exon 1
A1 - RUAN Wen-jing
A1 - LIN Jie
A1 - XU En-ping
A1 - XU Fang-ying
A1 - MA Yu
A1 - DENG Hong
A1 - HUANG Qiong
A1 - LV Bing-jian
A1 - HU Hu
A1 - CUI Jing
A1 - DI Mei-juan
A1 - DONG Jian-kang
A1 - LAI Mao-de
J0 - Journal of Zhejiang University Science B
VL - 7
IS - 11
SP - 929
EP - 932
%@ 1673-1581
Y1 - 2006
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2006.B0929


Abstract: 
Insulin-like growth factor binding-protein-7 (IGFBP7) was obtained from our previous colonic adenocarcinoma (CRC) and normal mucosa suppression subtraction hybridization (SSH) cDNA libraries. By RT-PCR and immunohistochemistry, we found that IGFBP7 was overexpressed in CRC tissue compared to normal tissue. However, our in vitro experiments performed in 10 CRC cell lines showed that IGFBP7 expressed only in SW480 and Caco2 cell lines, which implied an underlying reversible regulatory mechanism. Using methylation-specific PCR (MSP) and bisulfite sodium PCR (BSP), we found that its expression was associated with DNA hypomethylation of exon1. This was further supported by the in vitro study which showed restored IGFBP7 expression after demethylation agent 5-aza-2'-deoxycytidine treatment. Correlation analysis between IGFBP7 expression and prognosis indicated that overexpression of IGFBP7 in CRC tissue correlated with favourable survival. Investigation of the functional role of IGFBP7 through transfection studies showed that IGFBP7 protein could inhibit growth rate, decrease colony formation activity, and induce apoptosis in RKO and SW620 cells, suggesting it a potential tumor suppressor protein in colorectal carcinogenesis. In conclusion, our study clearly demonstrated that IGFBP7 plays a potential tumor suppressor role against colorectal carcinogenesis and its expression is associated with DNA hypomethylation of exon 1.

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