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Journal of Zhejiang University SCIENCE A 2007 Vol.8 No.9 P.1469~1474

http://doi.org/10.1631/jzus.2007.A1469


Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs


Author(s):  WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng

Affiliation(s):  Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   wudl@zju.edu.cn, pzheng@zju.edu.cn

Key Words:  Denitrifying bacteria, Arthrobacter sp. strain CW-1, Dimethyl phthalate (DMP), Phthalic acid (PA), DMP degradation pathway


WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng. Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs[J]. Journal of Zhejiang University Science A, 2007, 8(9): 1469~1474.

@article{title="Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs",
author="WU Dong-lei, ZHENG Ping, MAHMOOD Qaisar, YANG Xiao-sheng",
journal="Journal of Zhejiang University Science A",
volume="8",
number="9",
pages="1469~1474",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.A1469"
}

%0 Journal Article
%T Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs
%A WU Dong-lei
%A ZHENG Ping
%A MAHMOOD Qaisar
%A YANG Xiao-sheng
%J Journal of Zhejiang University SCIENCE A
%V 8
%N 9
%P 1469~1474
%@ 1673-565X
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.A1469

TY - JOUR
T1 - Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs
A1 - WU Dong-lei
A1 - ZHENG Ping
A1 - MAHMOOD Qaisar
A1 - YANG Xiao-sheng
J0 - Journal of Zhejiang University Science A
VL - 8
IS - 9
SP - 1469
EP - 1474
%@ 1673-565X
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.A1469


Abstract: 
Isolation of new bacterial strains and recognition of their metabolic activities are highly desirable for sustainability of natural ecosystems. Biodegradation of dimethyl phthalate (DMP) under anoxic conditions has been shown to occur as a series of sequential steps using strain CW-1 isolated from digested sludge of Sibao Wastewater Treatment Plant in Hangzhou, China. The microbial colony on LB medium was yellowish, 3~5 mm in diameter, convex in the center, and embedded in mucous externally. The individual cells of strain CW-1 are irregular rods, measuring (0.6~0.7)×(0.9~1.0) μm, V-shaped, with clubbed ends, Gram positive and without any filaments. 16S rDNA (1438 bp) sequence analysis showed that the strain was related to Arthrobacter sp. CW-1 and can degrade PAEs utilizing nitrate as electron acceptor, but cannot mineralize DMP completely. The degradation pathway was recommended as: dimethyl phthalate (DMP)→monomethyl phthalate (MMP)→phthalic acid (PA). DMP biodegradation was a first order reaction with degradation rate constant of 0.3033 d−1 and half-life 2.25 d. The DMP conversion to PA by CW-1 could be described by using sequential kinetic model.

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