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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.56-59

http://doi.org/10.1631/jzus.2007.B0056


Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers


Author(s):  ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong

Affiliation(s):  Institute of Pharmaceutical Engineering, Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   jie_0822@yahoo.com.cn

Key Words:  Fluoxetine, Chiral stationary phase, Enantiomeric separation, High performance liquid chromatography (HPLC)


ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong. Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers[J]. Journal of Zhejiang University Science B, 2007, 8(1): 56-59.

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author="ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong",
journal="Journal of Zhejiang University Science B",
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pages="56-59",
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publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0056"
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%T Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers
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%A YANG Yi-wen
%A WEI Feng
%A WU Ping-dong
%J Journal of Zhejiang University SCIENCE B
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0056

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T1 - Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers
A1 - ZHOU Jie
A1 - YANG Yi-wen
A1 - WEI Feng
A1 - WU Ping-dong
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 56
EP - 59
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0056


Abstract: 
Separation of fluoxetine enantiomers on five chiral stationary phases (chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond І 2000 DM and kromasil CHI-TBB) was investigated. The optimal mobile phase compositions of fluoxetine separation on each column were hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), hexane/isopropanol/diethyl amine (99/1/0.1, v/v/v), hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), methanol/0.2% triethylamine acetic acid (TEAA) (25/75, v/v; pH 3.8) and hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), respectively. Experimental results demonstrated that baseline separation (RS>1.5) of fluoxetine enantiomers was obtained on chiralcel OD-H, chiralpak AD-H, and cyclobond І 2000 DM while the best separation was obtained on the last one. The eluate orders of fluoxetine enantiomers on the columns were determined. The first eluate by chiralcel OJ-H and kromasil CHI-TBB is the S-enantiomer, while by chiralpak AD-H and cyclobond I 2000 DM is the R-enantiomer.

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