JZUS - Journal of Zhejiang University SCIENCE

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.56-59

http://doi.org/10.1631/jzus.2007.B0056

Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers

Author(s): ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong
Affiliation(s): Institute of Pharmaceutical Engineering, Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more
Corresponding email(s): jie_0822@yahoo.com.cn
Key Words: Fluoxetine, Chiral stationary phase, Enantiomeric separation, High performance liquid chromatography (HPLC)

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Abstract: Separation of fluoxetine enantiomers on five chiral stationary phases (chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond І 2000 DM and kromasil CHI-TBB) was investigated. The optimal mobile phase compositions of fluoxetine separation on each column were hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), hexane/isopropanol/diethyl amine (99/1/0.1, v/v/v), hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), methanol/0.2% triethylamine acetic acid (TEAA) (25/75, v/v; pH 3.8) and hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), respectively. Experimental results demonstrated that baseline separation (R_S>1.5) of fluoxetine enantiomers was obtained on chiralcel OD-H, chiralpak AD-H, and cyclobond І 2000 DM while the best separation was obtained on the last one. The eluate orders of fluoxetine enantiomers on the columns were determined. The first eluate by chiralcel OJ-H and kromasil CHI-TBB is the S-enantiomer, while by chiralpak AD-H and cyclobond I 2000 DM is the R-enantiomer.

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