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Received: 2003-06-10

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Journal of Zhejiang University SCIENCE A 2004 Vol.5 No.6 P.738~742

http://doi.org/10.1631/jzus.2004.0738


Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor


Author(s):  YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming

Affiliation(s):  First Affiliated Hospital, College of Medical Science, Zhejiang University, Hangzhou 310003, China

Corresponding email(s):   caohc70@yahoo.com.cn

Key Words:  Platelet activating factor, Reversed phase high-performance liquid chromatographic technique, Coronary heart disease, Cerebral infarction


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YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming. Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor[J]. Journal of Zhejiang University Science A, 2004, 5(6): 738~742.

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author="YANG Yun-mei, CAO Hong-cui, XU Zhe-rong, CHEN Xiao-ming",
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year="2004",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2004.0738"
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%T Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor
%A YANG Yun-mei
%A CAO Hong-cui
%A XU Zhe-rong
%A CHEN Xiao-ming
%J Journal of Zhejiang University SCIENCE A
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2004.0738

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T1 - Creation of reversed phase high-performance liquid chromatographic technique to assay platelet-activating factor
A1 - YANG Yun-mei
A1 - CAO Hong-cui
A1 - XU Zhe-rong
A1 - CHEN Xiao-ming
J0 - Journal of Zhejiang University Science A
VL - 5
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.2004.0738


Abstract: 
Objective: To establish a new assay for platelet-activating factor (PAF), to compare it with bio-assay; and to discuss its significance in some elderly people diseases such as cerebral infarction and coronary heart disease. Methods: To measure PAF levels in 100 controls, 23 elderly patients with cerebral infarction and 65 cases with coronary heart disease by reversed phase high-performance liquid chromatographic technique (rHPLC). Results: rHPLC is more convenient, sensitive, specific, and less confusing, compared with bio-assay. The level of plasma PAF in patients with cerebral infarction was higher than that in the controls (P<0.01), and in patients with coronary heart disease. Conclusion: Detection of PAF with rHPLC is more reliable and more accurate. The new assay has important significance in PAF research.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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[7] Silvestro, L., Da Col, R., Scappaticci, E., Libertucci, D., Biancone, L., Camussi, G., 1993. Development of a high performance liquid chromatographic-mass spectrometric technique, with an ionspray interface, for the determination of platelet-activating factor (PAF) and lyso-PAF in biological samples.Journal of chromatography,647(2):261-269.

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[9] Xu, W.R., Gu, K.L., Cao, H.C., Sun X.C., Cao, C.C., 1997. Detection of plasma lyso-platelet-activating factor and its application in Childhood asthma.Chinese Journal of Clinical Laboratory Science,15(5):265-268 (in Chinese).

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