Full Text:   <1653>

CLC number: O657.7

On-line Access: 

Received: 2005-11-01

Revision Accepted: 2006-02-14

Crosschecked: 0000-00-00

Cited: 2

Clicked: 4419

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.1 P.56~59

http://doi.org/10.1631/jzus.2007.B0056


Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers


Author(s):  ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong

Affiliation(s):  Institute of Pharmaceutical Engineering, Department of Chemical Engineering, Zhejiang University, Hangzhou 310027, China; more

Corresponding email(s):   jie_0822@yahoo.com.cn

Key Words:  Fluoxetine, Chiral stationary phase, Enantiomeric separation, High performance liquid chromatography (HPLC)


ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong. Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers[J]. Journal of Zhejiang University Science B, 2007, 8(1): 56~59.

@article{title="Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers",
author="ZHOU Jie, YANG Yi-wen, WEI Feng, WU Ping-dong",
journal="Journal of Zhejiang University Science B",
volume="8",
number="1",
pages="56~59",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0056"
}

%0 Journal Article
%T Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers
%A ZHOU Jie
%A YANG Yi-wen
%A WEI Feng
%A WU Ping-dong
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 1
%P 56~59
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0056

TY - JOUR
T1 - Comparison of the performance of chiral stationary phase for separation of fluoxetine enantiomers
A1 - ZHOU Jie
A1 - YANG Yi-wen
A1 - WEI Feng
A1 - WU Ping-dong
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 1
SP - 56
EP - 59
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0056


Abstract: 
Separation of fluoxetine enantiomers on five chiral stationary phases (chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond І 2000 DM and kromasil CHI-TBB) was investigated. The optimal mobile phase compositions of fluoxetine separation on each column were hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), hexane/isopropanol/diethyl amine (99/1/0.1, v/v/v), hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), methanol/0.2% triethylamine acetic acid (TEAA) (25/75, v/v; pH 3.8) and hexane/isopropanol/diethyl amine (98/2/0.2, v/v/v), respectively. Experimental results demonstrated that baseline separation (RS>1.5) of fluoxetine enantiomers was obtained on chiralcel OD-H, chiralpak AD-H, and cyclobond І 2000 DM while the best separation was obtained on the last one. The eluate orders of fluoxetine enantiomers on the columns were determined. The first eluate by chiralcel OJ-H and kromasil CHI-TBB is the S-enantiomer, while by chiralpak AD-H and cyclobond I 2000 DM is the R-enantiomer.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Agranat, I., Caner, H., 1999. Intellectual property and chirality of drugs. Drug Discov. Today, 4(7):313-321.

[2] Bakhtiar, R., Tse, F., 2000. High-throughput chiral liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 14(13):1128-1135.

[3] Berthod, A., Jin, H.L., Beesley, T.E., Duncan, J.D., Armstrong, D.W., 1990. Cyclodextrin chiral stationary phases for liquid chromatography separations of drug stereoisomers. J. Pharm. Biomed. Anal., 8(2):123-130.

[4] Eap, C.B., Gaillard, N., Powell, K., Baumann, P., 1996. Simultaneous determination of plasma levels of fluvoxamine and of the enantiomers of fluoxetine and norfluoxetine by gas chromatography-mass spectrometry. J. Chromatogr. B, 682:265-272.

[5] Gatti, G., Bonomi, I., Marchiselli, R., Fattore, C., Spina, E., Scordo, G., Pacifici, R., Perucca, E., 2003. Improved enantioselective assay for the determination of fluoxetine and norfluoxetine enantiomers in human plasmaby liquid chromatography. J. Chromatogr. B, 784(2):375-383.

[6] Guo, X., Fukushima, T., Li, F., Imai, K., 2002. Determination of fluoxetine enantiomers in rat plasma by pre-column fluorescence derivatization and column-switehing high-performance liquid chromatography. Analyst, 127(4):480-484.

[7] Guo, X.J., Xu, Y., Li, F.M., 2004. Enantiomeric separation of derivatized fluoxetine on chiralcel OD column. Chin. J. Anal. Chem., 32(10):1353-1355.

[8] Kaddoumi, A., Nakashima, M.N., Nakashima, K., 2001. Fluorometric determination of DL-fenfluramine, DL-norfenfluramine and phentermine in plasma by achiral and chiral high-performance liquid chromatography. J. Chromatogr. B, 763:79-90.

[9] Olsen, B.A., Wirth, D.D., Larew, J.S., 1998. Determination of fluoxetine hydrochloride enantiomeric excess using high-performance liquid chromatography with chiral stationary phases. J. Pharm. Biomed. Anal., 17(4-5):623-630.

[10] Piperaki, S., Poulou, M.P., 1993. Use of cyclodextrin as chiral selectors for direct resolution of the enantiomers of fluoxetine and its metabolite norfluoxetine by HPLC. Chirality, 5(4):258-266.

[11] Piperaki, S., Penn, S.G., Goodall, D.M., 1995. Systematic approach to treatment of enantiomeric separations in capillary electrophoresis and liquid chromatography II: a study of the enantiomeric separation of fluoxetine and norfluoxetin. J. Chromatogr. A, 700(1-2):59-67.

[12] Potts, B.D., Parli, C.J., 1992. Analysis of the enantiomers of fluoxetine and norfluoxetine in plasma and tissue using chiral derivatization and normal-phase liquid chromatography. J. Liq. Chromatogr., 15(4):665-681.

[13] Shen, Z., Wang, S., Bakhtiar, R., 2002. Enantiomeric separation and quantification of fluoxetine in human plasma by liquid chromatography/tandem mass spectrometry using liquid-liquid extraction in 96-well plate format. Rapid Commun. Mass Spectrom., 16(5):332-338.

[14] Torok-Both, G.A., Baker, G.B., Coutts, R.T., 1992. Simultaneous determination of fluoxetine and norfluoxetine enantiomers in biological samples by gas chromatography with electron-capture detection. J. Chromatogr., 579:99-106.

[15] Wang, Z.X., Yun, Z.H., 1997. Separation of fluoxetine enantiomers by HPLC with urea derivative as chiral stationary phase. Chin. J. Anal. Chem., 25(4):464-467.

[16] Wang, T.J., Li, J., Wang, Y., Guo, X.J., Zhang, C.H., Li, F.M., 2004. Enantiomeric separation of derivatized fluoxetine by HPLC on amylose stationary phase. Chin. J. Pharm. Anal., 24(3):318-320.

[17] Yee, L., Wong, S., Skrinska, V., 2000. Chiral high-performance liquid chromatography analysis of fluoxetine and norfluoxetine in rabbit plasma, urine and vitreous humor using an acetylated β-cyclodextrin column. J. Anal. Toxicol., 24:651-655.

[18] Yu, H.W., Ching, C.B., Fu, P., Ng, S.C., 2002. Enantioseparation of fluoxetine on a new β-cyclodextrin bonded phase column by HPLC. Separation Sci. Technol., 37(6):1401-1415.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE