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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.365~370

10.1631/jzus.2007.B0365


Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma


Author(s):  NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing

Affiliation(s):  School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   nihui@jmu.edu.cn, gqhe@zju.edu.cn

Key Words:  Phaffia rhodozyma, Astaxanthin, Nitrogen source, Optimization


NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing. Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma[J]. Journal of Zhejiang University Science B, 2007, 8(5): 365~370.

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author="NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing",
journal="Journal of Zhejiang University Science B",
volume="8",
number="5",
pages="365~370",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0365"
}

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%T Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma
%A NI Hui
%A CHEN Qi-he
%A RUAN Hui
%A YANG Yuan-fan
%A LI Li-jun
%A WU Guang-bin
%A HU Yang
%A HE Guo-qing
%J Journal of Zhejiang University SCIENCE B
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%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0365

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T1 - Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma
A1 - NI Hui
A1 - CHEN Qi-he
A1 - RUAN Hui
A1 - YANG Yuan-fan
A1 - LI Li-jun
A1 - WU Guang-bin
A1 - HU Yang
A1 - HE Guo-qing
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 365
EP - 370
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0365


Abstract: 
Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using (NH4)2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L (NH4)2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

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