Full Text:   <3498>

CLC number: Q939

On-line Access: 2024-08-27

Received: 2023-10-17

Revision Accepted: 2024-05-08

Crosschecked: 0000-00-00

Cited: 14

Clicked: 6038

Citations:  Bibtex RefMan EndNote GB/T7714

-   Go to

Article info.
1. Reference List
Open peer comments

Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.5 P.365-370

http://doi.org/10.1631/jzus.2007.B0365


Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma


Author(s):  NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing

Affiliation(s):  School of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China; more

Corresponding email(s):   nihui@jmu.edu.cn, gqhe@zju.edu.cn

Key Words:  Phaffia rhodozyma, Astaxanthin, Nitrogen source, Optimization


NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing. Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma[J]. Journal of Zhejiang University Science B, 2007, 8(5): 365-370.

@article{title="Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma",
author="NI Hui, CHEN Qi-he, RUAN Hui, YANG Yuan-fan, LI Li-jun, WU Guang-bin, HU Yang, HE Guo-qing",
journal="Journal of Zhejiang University Science B",
volume="8",
number="5",
pages="365-370",
year="2007",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.2007.B0365"
}

%0 Journal Article
%T Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma
%A NI Hui
%A CHEN Qi-he
%A RUAN Hui
%A YANG Yuan-fan
%A LI Li-jun
%A WU Guang-bin
%A HU Yang
%A HE Guo-qing
%J Journal of Zhejiang University SCIENCE B
%V 8
%N 5
%P 365-370
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0365

TY - JOUR
T1 - Studies on optimization of nitrogen sources for astaxanthin production by Phaffia rhodozyma
A1 - NI Hui
A1 - CHEN Qi-he
A1 - RUAN Hui
A1 - YANG Yuan-fan
A1 - LI Li-jun
A1 - WU Guang-bin
A1 - HU Yang
A1 - HE Guo-qing
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 5
SP - 365
EP - 370
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0365


Abstract: 
Fermentation of Phaffia rhodozyma is a major method for producing astaxanthin, an important pigment with industrial and pharmaceutical application. To improve astaxanthin productivity, single factor and mixture design experiments were used to investigate the effects of nitrogen source on Phaffia rhodozyma cultivation and astaxanthin production. Results of single factor experiments showed nitrogen source could significantly affect P. rhodozyma cultivation with respect to carbon source utilization, yeast growth and astaxanthin accumulation. Further studies of mixture design experiments using (NH4)2SO4, KNO3 and beef extract as nitrogen sources indicated that the proportion of three nitrogen sources was very important to astaxanthin production. Validation experiments showed that the optimal nitrogen source was composed of 0.28 g/L (NH4)2SO4, 0.49 g/L KNO3 and 1.19 g/L beef extract. The kinetic characteristics of batch cultivation were investigated in a 5-L pH-stat fermentor. The maximum amount of biomass and highest astaxanthin yield in terms of volume and in terms of biomass were 7.71 mg/L and 1.00 mg/g, respectively.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1] Du, X.L., Zhu, M.J., Wu, Z.Q., Liang, S.Z., 2005. Effects of nitrogen sources on astaxanthin production by Phaffia rhodozyma. Sci. Technol. Food Ind., 26(9):73-75, 77 (in Chinese).

[2] Guerin, M., Huntley, M.E., Olaizola, M., 2003. Haematococcus astaxanthin: application for human health and nutrition. Trends Biotechnol., 21(5):210-216.

[3] Hu, Z.C., Zheng, Y.G., Wang, Z., Shen, Y.C., 2005. Effect of sugar-feeding strategies on astaxanthin production by Xanthophyllomyces dendrorhous. World J. Microbiol. Biotechnol., 21(5):771-775.

[4] Jesus, R., Humberto, G., Anne, G., 2001. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology. J. Biothechnol., 88:259-268.

[5] Johnson, E.A., 1991. Astaxanthin from microbiol sources. Crit. Rev. Biotechnol., 11(4):297-326.

[6] Johnson, E.A., 2003. Phaffia rhodozyma: colorful odyssey. Int. Microbiol., 6(3):169-174.

[7] Kusdiyantini, E., Gaudin, P., Goma, G., Blanc, P.J., 1998. Growth kinetics and astaxanthin production of Phaffia rhodozyma on glycerol as a carbon source during batch fermentation. Biotechnol. Lett., 20(10):929-934.

[8] Montgomery, D.C., 1997. Design and Analysis of Experiments, 4th Ed. John Wiley & Sons Inc., New York.

[9] Ni, H., He, G.Q., Yang, Y.F., Wu, G.B., Chen, S.R., Cai, L.S., 2004. Optimization of condition for extracting astaxanthin from Phaffia rhodozyma. Trans. Chin. Soc. Agric. Eng., 20(2):204-208 (in Chinese).

[10] Ni, H., He, G.Q., Ruan, H., Chen, Q.H., Chen, F., 2005a. Application of derivative ratio spectrophotometry for the determination of β-carotene and astaxanthin from the Phaffia rhodozyma extract. J. Zhejiang Univ. Sci., 6B(6):514-522.

[11] Ni, H., Yang, Y.F., Yang, Q.M., Guo, C.H., Cai, H.N., 2005b. Studies on the determination of carotenoids of Phaffia rhodozyma by spectrophotometric method. J. Jimei Univ. (Nat. Sci.), 10(4):318-323 (in Chinese).

[12] Ni, H., He, G.Q., Yang, Y.F., Cai, H.N., 2005c. Studies on effects of metal ions on astaxanthin production by Phaffia rhodozyma. Food Fermentation Ind., 31(12):4-7 (in Chinese).

[13] Paul, D.F., Yutaka, M., Misawa, N., 1997. In vitro characterization of astaxanthin biosynthetic enzymes. J. Bioeng. Chem., 272(10):6128-6135.

[14] Sedmak, J.J., Weerasinghe, D.K., Jolly, S.O., 1990. Extraction and quantification of astaxanthin from Phaffia rhodozyma. Biotechnol. Tech., 4(2):107-112.

[15] Verdoes, J.C., Misawa, N., van Ooyen, A.J.J., 1999. Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of Xanthphyllomyces dendrorhous. Biotechnol. Bioeng., 63(6):750-755.

[16] Wang, Q.Y., Li, Q.S., 1997. Natural Carotenoids: Advancements of Studies, Production and Applications. China Press of Pharmaceutical Science and Technology, Beijing, China (in Chinese).

[17] Zhang, L.X., Zhang, T.F., Li, L.Y., 1997. Methodology of Biochemical. Higher Education Press, Beijing, China (in Chinese).

[18] Zhu, M.J., Liang, S.Z., 2005. Optimizations culture parameter for growth and astaxanthin accumulation by Phaffia rhodozyma. J. Zhengzhou Univ. Light Ind., 20(2):17-19 (in Chinese).

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - 2024 Journal of Zhejiang University-SCIENCE