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Journal of Zhejiang University SCIENCE B 2007 Vol.8 No.11 P.831-838


Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5

Author(s):  ZHU Xu-fen, ZHOU Ying, FENG Jun-li

Affiliation(s):  College of Life Science, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   zhuxufen@mail.hz.zj.cn

Key Words:  Sphingomonas sp., Chitinase, Chitosanase

ZHU Xu-fen, ZHOU Ying, FENG Jun-li. Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5[J]. Journal of Zhejiang University Science B, 2007, 8(11): 831-838.

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author="ZHU Xu-fen, ZHOU Ying, FENG Jun-li",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5
%A ZHU Xu-fen
%A ZHOU Ying
%A FENG Jun-li
%J Journal of Zhejiang University SCIENCE B
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%N 11
%P 831-838
%@ 1673-1581
%D 2007
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.2007.B0831

T1 - Analysis of both chitinase and chitosanase produced by Sphingomonas sp. CJ-5
A1 - ZHU Xu-fen
A1 - ZHOU Ying
A1 - FENG Jun-li
J0 - Journal of Zhejiang University Science B
VL - 8
IS - 11
SP - 831
EP - 838
%@ 1673-1581
Y1 - 2007
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.2007.B0831

A novel chitinolytic and chitosanolytic bacterium, Sphingomonas sp. CJ-5, has been isolated and characterized. It secretes both chitinase and chitosanase into surrounding medium in response to chitin or chitosan induction. To characterize the enzymes, both chitinase and chitosanase were purified by ammonium sulfate precipitation, Sephadex G-200 gel filtration and DEAE-Sepharose Fast Flow. SDS-PAGE analysis demonstrated molecular masses of chitinase and chitosanase were 230 kDa and 45 kDa respectively. The optimum hydrolysis conditions for chitinase were about pH 7.0 and 36 °C, and these for chitosanase were pH 6.5 and 56 °C, respectively. Both enzymes were quite stable up to 45 °C for one hour at pH 5~8. These results show that CJ-5 may have potential for industrial application particularly in recycling of chitin wastes.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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