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CLC number: X703.1; DQ89

On-line Access: 2008-05-29

Received: 2008-01-02

Revision Accepted: 2008-04-17

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Journal of Zhejiang University SCIENCE A 2008 Vol.9 No.7 P.1004~1010

10.1631/jzus.A0820064


Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis


Author(s):  Jian-fang WANG, Qing-liang ZHAO, Wen-biao JIN, Shi-jie YOU, Jin-na ZHANG

Affiliation(s):  School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China; more

Corresponding email(s):   wjf302@163.com, qlzhao@hit.edu.cn

Key Words:  Excess sludge reduction, Biological phosphorus removal, Phosphate accumulating organisms (PAOs), DAPI (4&prime


Jian-fang WANG, Qing-liang ZHAO, Wen-biao JIN, Shi-jie YOU, Jin-na ZHANG. Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis[J]. Journal of Zhejiang University Science A, 2008, 9(7): 1004~1010.

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author="Jian-fang WANG, Qing-liang ZHAO, Wen-biao JIN, Shi-jie YOU, Jin-na ZHANG",
journal="Journal of Zhejiang University Science A",
volume="9",
number="7",
pages="1004~1010",
year="2008",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.A0820064"
}

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%T Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis
%A Jian-fang WANG
%A Qing-liang ZHAO
%A Wen-biao JIN
%A Shi-jie YOU
%A Jin-na ZHANG
%J Journal of Zhejiang University SCIENCE A
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%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.A0820064

TY - JOUR
T1 - Performance of biological phosphorus removal and characteristics of microbial community in the oxic-settling-anaerobic process by FISH analysis
A1 - Jian-fang WANG
A1 - Qing-liang ZHAO
A1 - Wen-biao JIN
A1 - Shi-jie YOU
A1 - Jin-na ZHANG
J0 - Journal of Zhejiang University Science A
VL - 9
IS - 7
SP - 1004
EP - 1010
%@ 1673-565X
Y1 - 2008
PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.A0820064


Abstract: 
Performance of biological phosphorus removal in the oxic-settling-anaerobic (OSA) process was investigated. Cell staining and fluorescent in situ hybridization (FISH) were used to analyze characteristics and microbial community of sludge. Experimental results showed that phosphorus removal efficiency was near 60% and the amount of biological phosphorus accumulation in aerobic sludge of the OSA system was up to 26.9 mg/g. biological phosphorus removal efficiency was partially inhibited by carbon sources in the continuous OSA system. Contrasted to the OSA system, biological phosphorus removal efficiency was enhanced by 14% and the average total phosphorus (TP) contents of aerobic sludge were increased by 0.36 mg/g when sufficient carbon sources were supplied in batch experiments. Staining methods indicated that about 35% of microorganisms had typical characteristics of phosphorus accumulating organisms (PAOs). FISH analysis demonstrated that PAOMIX-binding bacteria were predominant microbial communities in the OSA system, which accounted for around 28% of total bacteria.

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