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Journal of Zhejiang University SCIENCE B 2009 Vol.10 No.5 P.368~374

10.1631/jzus.B0820285


Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease


Author(s):  Zhi-qiang YING, Dan-dan ZHONG, Geng XU, Miao-yan CHEN, Qing-yu CHEN

Affiliation(s):  Department of Cardiology, the Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China; more

Corresponding email(s):   xugengdoctor@sina.com

Key Words:  Adiponectin, Adipokines, Endothelial progenitor cells (EPCs), Coronary artery disease (CAD)


Zhi-qiang YING, Dan-dan ZHONG, Geng XU, Miao-yan CHEN, Qing-yu CHEN. Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease[J]. Journal of Zhejiang University Science B, 2009, 10(5): 368~374.

@article{title="Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease",
author="Zhi-qiang YING, Dan-dan ZHONG, Geng XU, Miao-yan CHEN, Qing-yu CHEN",
journal="Journal of Zhejiang University Science B",
volume="10",
number="5",
pages="368~374",
year="2009",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B0820285"
}

%0 Journal Article
%T Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease
%A Zhi-qiang YING
%A Dan-dan ZHONG
%A Geng XU
%A Miao-yan CHEN
%A Qing-yu CHEN
%J Journal of Zhejiang University SCIENCE B
%V 10
%N 5
%P 368~374
%@ 1673-1581
%D 2009
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B0820285

TY - JOUR
T1 - Adiponectin levels are associated with the number and activity of circulating endothelial progenitor cells in patients with coronary artery disease
A1 - Zhi-qiang YING
A1 - Dan-dan ZHONG
A1 - Geng XU
A1 - Miao-yan CHEN
A1 - Qing-yu CHEN
J0 - Journal of Zhejiang University Science B
VL - 10
IS - 5
SP - 368
EP - 374
%@ 1673-1581
Y1 - 2009
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B0820285


Abstract: 
Objective: To study the relationship between plasma adiponectin concentration and the functional activities of circulating endothelial progenitor cells (EPCs) in patients with coronary artery disease (CAD). Methods: Circulating EPCs were enumerated as AC133+/KDR+ cells via flow cytometry and identified by co-staining with DiI-acLDL and fluorescein isothiocyanate (FITC)-conjugated lectin under a fluorescent microscope. The migratory capacity of EPCs was measured by modified Boyden chamber assay. Adhesion capacity was performed to count adherent cells after replating EPCs on six-well culture dishes coated with fibronectin. Results: The number of circulating EPCs (AC133+/KDR+ cells) decreased significantly in CAD patients, compared with control subjects [(74.2±12.3) vs (83.5±12.9) cells/ml blood, P<0.01]. In addition, the number of EPCs also decreased in CAD patients after ex vivo cultivation [(54.4±8.6) vs (71.9±11.6) EPCs/field, P<0.01]. Both circulating EPCs and differentiated EPCs were positively correlated with plasma adiponectin concentration. The functional activities of EPCs from CAD patients, such as migratory and adherent capacities, were also impaired, compared with control subjects, and positively correlated with plasma adiponectin concentration. Conclusion: The study demonstrates that the impairment of the number and functional activities of EPCs in CAD patients is correlated with their lower plasma adiponectin concentrations.

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