CLC number: R917
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2011-01-26
Cited: 6
Clicked: 6329
Shu-fang Wang, Hai-yan Fang, Hai-bin Qu. Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule[J]. Journal of Zhejiang University Science B, 2011, 12(3): 193-200.
@article{title="Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule",
author="Shu-fang Wang, Hai-yan Fang, Hai-bin Qu",
journal="Journal of Zhejiang University Science B",
volume="12",
number="3",
pages="193-200",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000135"
}
%0 Journal Article
%T Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule
%A Shu-fang Wang
%A Hai-yan Fang
%A Hai-bin Qu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 3
%P 193-200
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000135
TY - JOUR
T1 - Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule
A1 - Shu-fang Wang
A1 - Hai-yan Fang
A1 - Hai-bin Qu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 3
SP - 193
EP - 200
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000135
Abstract: central composite design (CCD), together with multiple linear regression, was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography (MEKC) for the determination of albiflorin, paeoniflorin, liquiritin, and glycyrrhizic acid in the traditional Chinese medicine (TCM) prescription, yangwei granule. Concentrations of sodium deoxycholate (SDC) and borate, and proportions of ammonia, acetonitrile, and methanol were optimized. The total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation efficiency. The optimum electrophoretic buffer contained 80 mmol/L SDC, 20 mmol/L borate, 5% (v/v) methanol, 0.5% (v/v) ammonia, and 5% (v/v) acetonitrile. The correlation coefficients (R2) between the peak areas and the corresponding concentrations of analytes were greater than 0.9956. The limits of detection (LODs) (S/N=3) of the analytes were 0.97–4.00 μg/ml. The results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.
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