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CLC number: R917

On-line Access: 2011-03-09

Received: 2010-04-14

Revision Accepted: 2010-05-31

Crosschecked: 2011-01-26

Cited: 6

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.3 P.193-200

http://doi.org/10.1631/jzus.B1000135


Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule


Author(s):  Shu-fang Wang, Hai-yan Fang, Hai-bin Qu

Affiliation(s):  Pharmaceutical Informatics Institute, Zhejiang University, Hangzhou 310058, China

Corresponding email(s):   quhb@zju.edu.cn

Key Words:  Micellar electrokinetic capillary chromatography, Traditional Chinese medicine, Central composite design, Yangwei granule


Shu-fang Wang, Hai-yan Fang, Hai-bin Qu. Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule[J]. Journal of Zhejiang University Science B, 2011, 12(3): 193-200.

@article{title="Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule",
author="Shu-fang Wang, Hai-yan Fang, Hai-bin Qu",
journal="Journal of Zhejiang University Science B",
volume="12",
number="3",
pages="193-200",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000135"
}

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%T Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule
%A Shu-fang Wang
%A Hai-yan Fang
%A Hai-bin Qu
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 3
%P 193-200
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000135

TY - JOUR
T1 - Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule
A1 - Shu-fang Wang
A1 - Hai-yan Fang
A1 - Hai-bin Qu
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 3
SP - 193
EP - 200
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000135


Abstract: 
central composite design (CCD), together with multiple linear regression, was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography (MEKC) for the determination of albiflorin, paeoniflorin, liquiritin, and glycyrrhizic acid in the traditional Chinese medicine (TCM) prescription, yangwei granule. Concentrations of sodium deoxycholate (SDC) and borate, and proportions of ammonia, acetonitrile, and methanol were optimized. The total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation efficiency. The optimum electrophoretic buffer contained 80 mmol/L SDC, 20 mmol/L borate, 5% (v/v) methanol, 0.5% (v/v) ammonia, and 5% (v/v) acetonitrile. The correlation coefficients (R2) between the peak areas and the corresponding concentrations of analytes were greater than 0.9956. The limits of detection (LODs) (S/N=3) of the analytes were 0.97–4.00 μg/ml. The results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.

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Reference

[1]Ben Hameda, A., Gajdoova, D., Havel, J., 2006. Analysis of Salvia officinalis plant extracts by capillary electrophoresis. J. Sep. Sci., 29(8):1188-1192.

[2]Feng, W., Wang, W.Q., Zhao, P.Y., 2008. Dynamics study of active ingredients and nutrients in licorice. China J. Chin. Mat. Med., 33(10):1206-1207 (in Chinese).

[3]Gu, M.C., Qian, Y.F., Lv, G.Y., 2006. Advance in Study on Radix Paeoniae Alba. Bull. Sci. Technol., 22(3):337-342 (in Chinese).

[4]Hanrahan, G., Montes, R., Gomez, F.A., 2008. Chemometric experimental design based optimization techniques in capillary electrophoresis: a critical review of modern applications. Anal. Bioanal. Chem., 390(1):169-179.

[5]Lin, Z.Y., 2006. Studies on the Methodology of Optimizing Analytical Condition of Capillary Electrophoresis. MS Thesis, Zhejiang University, Hangzhou, China, p.1-9 (in Chinese).

[6]Liu, H., Wen, Y., Luan, F., Gao, Y., 2009. Application of experimental design and radial basis function neural network to the separation and determination of active components in traditional Chinese medicines by capillary electrophoresis. Anal. Chim. Acta, 638(1):88-93.

[7]Liu, J.J., Li, S.P., Wang, Y.T., 2006. Optimization for quantitative determination of four flavonoids in Epimedium by capillary zone electrophoresis coupled with diode array detection using central composite design. J. Chromatogr. A, 1103(2):344-349.

[8]Liu, L.L., Gu, Z.R., Si, D.D., 2008. Optimization for quantitative determination of rebaudioside a and stevioside in stevia by capillary zone electrophoresis using central composite design. J. Instr. Anal., 8(27):870-873 (in Chinese).

[9]Servais, A.C., Fillet, M., Chiap, P., Dewé, W., Hubert, P., Crommen, J., 2004. Enantiomeric separation of basic compounds using heptakis(2,3-di-O-methyl-6-O-sulfo)-β-cyclodextrin in combination with potassium camphorsulfonate in nonaqueous capillary electrophoresis: optimization by means of an experimental design. Electrophoresis, 25(16):2701-2710.

[10]Shaban, E., Dagmar, G., Josef, H., 2006. A new capillary zone electrophoresis method for the analysis of Ginkgo biloba extracts and Ginkgo biloba pharmaceutical products. J. Sep. Sci., 29(8):1174-1179.

[11]Wang, J., Shao, Q., Qu, H.B., Chen, Y.Y., Wu, X.Z., 2006. HPLC determination of four active components in sugar-free Yang Wei Granules. Chin. J. Pharm. Anal., 26(12):1804-1806 (in Chinese).

[12]Wu, H.K., Sheu, S.J., 1996. Capillary electrophoretic determination of the constituents of Paeoniae Radix. J. Chromatogr. A, 753(1):139-146.

[13]Yu, K., Lin, Z.Y., Cheng, Y.Y., 2006a. An approach to optimizing the separation of Chinese medicines by capillary electrophoresis using particle swarm optimization. Chin. J. Anal. Chem, 34(7):963-966 (in Chinese).

[14]Yu, K., Wang, Y.W., Cheng, Y.Y., 2006b. Determination of paeonol and paeoniflorin in Chinese medicine Cortex Moutan and ‘Shuangdan’ granule by micellar electrokinetic capillary chromatography. J. Pharm. Biomed. Anal., 40(5):1257-1262.

[15]Yu, K., Lin, Z.Y., Cheng, Y.Y., 2006c. Optimization of the buffer system of micellar electrokinetic capillary chromatography for the separation of the active components in Chinese medicine ‘Shuangdan’ granule by genetic algorithm. Anal. Chim. Acta, 562(1):66-72.

[16]Yu, K., Gong, Y., Lin, Z., Cheng, Y., 2007. Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Scutellaria baicalensis Georgi using capillary electrophoresis. J. Pharm. Biomed. Anal., 43(2):540-548.

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