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On-line Access: 2011-06-07

Received: 2010-07-21

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.6 P.468-476

http://doi.org/10.1631/jzus.B1000280


Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite


Author(s):  Xing-hua Zhou, Feng-na Xi, Yi-ming Zhang, Xian-fu Lin

Affiliation(s):  Department of Chemistry, Zhejiang University, Hangzhou 310027, China

Corresponding email(s):   llc123@zju.edu.cn

Key Words:  Reagentless biosensor, Layer-by-layer assembly, Horseradish peroxidase-methylene blue (HRP-MB) biocomposite, Functional multiwall carbon nanotubes, Hydrogen peroxide


Xing-hua Zhou, Feng-na Xi, Yi-ming Zhang, Xian-fu Lin. Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite[J]. Journal of Zhejiang University Science B, 2011, 12(6): 468-476.

@article{title="Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite",
author="Xing-hua Zhou, Feng-na Xi, Yi-ming Zhang, Xian-fu Lin",
journal="Journal of Zhejiang University Science B",
volume="12",
number="6",
pages="468-476",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000280"
}

%0 Journal Article
%T Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite
%A Xing-hua Zhou
%A Feng-na Xi
%A Yi-ming Zhang
%A Xian-fu Lin
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 6
%P 468-476
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000280

TY - JOUR
T1 - Reagentless biosensor based on layer-by-layer assembly of functional multiwall carbon nanotubes and enzyme-mediator biocomposite
A1 - Xing-hua Zhou
A1 - Feng-na Xi
A1 - Yi-ming Zhang
A1 - Xian-fu Lin
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 6
SP - 468
EP - 476
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000280


Abstract: 
A simple and controllable layer-by-layer (LBL) assembly method was proposed for the construction of reagentless biosensors based on electrostatic interaction between functional multiwall carbon nanotubes (MWNTs) and enzyme-mediator biocomposites. The carboxylated MWNTs were wrapped with polycations poly(allylamine hydrochloride) (PAH) and the resulting PAH-MWNTs were well dispersed and positively charged. As a water-soluble dye methylene blue (MB) could mix well with horseradish peroxidase (HRP) to form a biocompatible and negatively-charged HRP-MB biocomposite. A (PAH-MWNTs/HRP-MB)n bionanomultilayer was then prepared by electrostatic LBL assembly of PAH-MWNTs and HRP-MB on a polyelectrolyte precursor film-modified Au electrode. Due to the excellent biocompatibility of HRP-MB biocomposite and the uniform LBL assembly, the immobilized HRP could retain its natural bioactivity and MB could efficiently shuttle electrons between HRP and the electrode. The incorporation of MWNTs in the bionanomultilayer enhanced the surface coverage concentration of the electroactive enzyme and increased the catalytic current response of the electrode. The proposed biosensor displayed a fast response (2 s) to hydrogen peroxide with a low detection limit of 2.0×10−7 mol/L (S/N=3). This work provided a versatile platform in the further development of reagentless biosensors.

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