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On-line Access: 2011-11-04

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Journal of Zhejiang University SCIENCE B 2011 Vol.12 No.11 P.935-942

10.1631/jzus.B1000417


DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance


Author(s):  Jie-hong Zhao, Ji-shun Zhang, Yi Wang, Ren-gang Wang, Chun Wu, Long-jiang Fan, Xue-liang Ren

Affiliation(s):  Guizhou Institute of Tobacco Science, Guiyang 550081, China, Department of Agronomy, Zhejiang University, Hangzhou 310029, China

Corresponding email(s):   renxuel@126.com

Key Words:  Methylation-sensitive amplified polymorphism, Epigenetic modification, Tobacco mosaic virus (TMV) resistance, Flue-cured tobacco


Jie-hong Zhao, Ji-shun Zhang, Yi Wang, Ren-gang Wang, Chun Wu, Long-jiang Fan, Xue-liang Ren. DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance[J]. Journal of Zhejiang University Science B, 2011, 12(11): 935-942.

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author="Jie-hong Zhao, Ji-shun Zhang, Yi Wang, Ren-gang Wang, Chun Wu, Long-jiang Fan, Xue-liang Ren",
journal="Journal of Zhejiang University Science B",
volume="12",
number="11",
pages="935-942",
year="2011",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000417"
}

%0 Journal Article
%T DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance
%A Jie-hong Zhao
%A Ji-shun Zhang
%A Yi Wang
%A Ren-gang Wang
%A Chun Wu
%A Long-jiang Fan
%A Xue-liang Ren
%J Journal of Zhejiang University SCIENCE B
%V 12
%N 11
%P 935-942
%@ 1673-1581
%D 2011
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000417

TY - JOUR
T1 - DNA methylation polymorphism in flue-cured tobacco and candidate markers for tobacco mosaic virus resistance
A1 - Jie-hong Zhao
A1 - Ji-shun Zhang
A1 - Yi Wang
A1 - Ren-gang Wang
A1 - Chun Wu
A1 - Long-jiang Fan
A1 - Xue-liang Ren
J0 - Journal of Zhejiang University Science B
VL - 12
IS - 11
SP - 935
EP - 942
%@ 1673-1581
Y1 - 2011
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000417


Abstract: 
DNA methylation plays an important role in the epigenetic regulation of gene expression during plant growth, development, and polyploidization. However, there is still no distinct evidence in tobacco regarding the distribution of the methylation pattern and whether it contributes to qualitative characteristics. We studied the levels and patterns of methylation polymorphism at CCGG sites in 48 accessions of allotetraploid flue-cured tobacco, Nicotiana tabacum, using a methylation-sensitive amplified polymorphism (MSAP) technique. The results showed that methylation existed at a high level among tobacco accessions, among which 49.3% sites were methylated and 69.9% allelic sites were polymorphic. A cluster analysis revealed distinct patterns of geography-specific groups. In addition, three polymorphic sites significantly related to tobacco mosaic virus (TMV) resistance were explored. This suggests that tobacco breeders should pay more attention to epigenetic traits.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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