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CLC number: R187+.2; X592

On-line Access: 2012-04-06

Received: 2011-09-08

Revision Accepted: 2011-11-30

Crosschecked: 2012-03-13

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.4 P.254-260

http://doi.org/10.1631/jzus.B1100289


Decontamination of Bacillus subtilis var. niger spores on selected surfaces by chlorine dioxide gas


Author(s):  Yan-ju Li, Neng Zhu, Hai-quan Jia, Jin-hui Wu, Ying Yi, Jian-cheng Qi

Affiliation(s):  School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China; more

Corresponding email(s):   liyj@npec.org.cn, qijch@npec.org.cn

Key Words:  Chlorine dioxide gas, Bacillus subtilis var. niger spores, Decontamination


Yan-ju Li, Neng Zhu, Hai-quan Jia, Jin-hui Wu, Ying Yi, Jian-cheng Qi. Decontamination of Bacillus subtilis var. niger spores on selected surfaces by chlorine dioxide gas[J]. Journal of Zhejiang University Science B, 2012, 13(4): 254-260.

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author="Yan-ju Li, Neng Zhu, Hai-quan Jia, Jin-hui Wu, Ying Yi, Jian-cheng Qi",
journal="Journal of Zhejiang University Science B",
volume="13",
number="4",
pages="254-260",
year="2012",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1100289"
}

%0 Journal Article
%T Decontamination of Bacillus subtilis var. niger spores on selected surfaces by chlorine dioxide gas
%A Yan-ju Li
%A Neng Zhu
%A Hai-quan Jia
%A Jin-hui Wu
%A Ying Yi
%A Jian-cheng Qi
%J Journal of Zhejiang University SCIENCE B
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%N 4
%P 254-260
%@ 1673-1581
%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1100289

TY - JOUR
T1 - Decontamination of Bacillus subtilis var. niger spores on selected surfaces by chlorine dioxide gas
A1 - Yan-ju Li
A1 - Neng Zhu
A1 - Hai-quan Jia
A1 - Jin-hui Wu
A1 - Ying Yi
A1 - Jian-cheng Qi
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 4
SP - 254
EP - 260
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1100289


Abstract: 
Objective: Chlorine dioxide (CD) gas has been used as a fumigant in the disinfection of biosafety laboratories. In this study, some experiments were conducted to assess the inactivation of spores inoculated on six materials [stainless steel (SS), painted steel (PS), polyvinyl chlorid (PVC), polyurethane (PU), glass (GS), and cotton cloth (CC)] by CD gas. The main aims of the study were to determine the sporicidal efficacy of CD gas and the effect of prehumidification before decontamination on sporicidal efficacy. Methods: Material coupons (1.2 cm diameter of SS, PS, and PU; 1.0 cm×1.0 cm for PVC, GS, and CC) were contaminated with 10 μl of Bacillus subtilis var. niger (ATCC 9372) spore suspension in mixed organic burden and then dried in a biosafety cabinet for 12 h. The spores were recovered by soaking the coupons in 5 ml of extraction liquid for 1 h and then vortexing the liquid for 1 min. Results: The log reductions in spore numbers on inoculated test materials exposed to CD gas [0.080% (volume ratio, v/v) for 3 h] were in the range of from 1.80 to 6.64. Statistically significant differences were found in decontamination efficacies on test material coupons of SS, PS, PU, and CC between with and without a 1-h prehumidification treatment. With the extraction method, there were no statistically significant differences in the recovery ratios between the porous and non-porous materials. Conclusions: The results reported from this study could provide information for developing decontamination technology based on CD gas for targeting surface microbial contamination.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

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