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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.7 P.511-524


Conditional gene manipulation: Cre-ating a new biological era

Author(s):  Jian Zhang, Jing Zhao, Wen-jie Jiang, Xi-wei Shan, Xiao-mei Yang, Jian-gang Gao

Affiliation(s):  School of Life Science, Shandong University, Jinan 250100, China

Corresponding email(s):   yxm411@sdu.edu.cn, jggao@sdu.edu.cn

Key Words:  Site-specific recombinase, Gene targeting, Gene trapping, Inducible systems, Φ, C31 system

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Jian Zhang, Jing Zhao, Wen-jie Jiang, Xi-wei Shan, Xiao-mei Yang, Jian-gang Gao. Conditional gene manipulation: Cre-ating a new biological era[J]. Journal of Zhejiang University Science B, 2012, 13(7): 511-524.

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author="Jian Zhang, Jing Zhao, Wen-jie Jiang, Xi-wei Shan, Xiao-mei Yang, Jian-gang Gao",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Conditional gene manipulation: Cre-ating a new biological era
%A Jian Zhang
%A Jing Zhao
%A Wen-jie Jiang
%A Xi-wei Shan
%A Xiao-mei Yang
%A Jian-gang Gao
%J Journal of Zhejiang University SCIENCE B
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%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200042

T1 - Conditional gene manipulation: Cre-ating a new biological era
A1 - Jian Zhang
A1 - Jing Zhao
A1 - Wen-jie Jiang
A1 - Xi-wei Shan
A1 - Xiao-mei Yang
A1 - Jian-gang Gao
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 7
SP - 511
EP - 524
%@ 1673-1581
Y1 - 2012
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200042

To solve the problem of embryonic lethality in conventional gene knockouts, site-specific recombinase (SSR) systems (Cre-loxP, Flp-FRT, and Φ;C31) have been used for tissue-specific gene knockout. With the combination of an SSR system and inducible gene expression systems (tetracycline and tamoxifen), stage-specific knockout and transgenic expression can be achieved. The application of this “SSR+inducible” conditional tool to genomic manipulation can be extended in various ways. Alternatives to conditional gene targeting, such as conditional gene trapping, multipurpose conditional alleles, and conditional gene silencing, have been developed. SSR systems can also be used to construct precise disease models with point mutations and chromosomal abnormalities. With these exciting achievements, we are moving towards a new era in which the whole genome can be manipulated as we wish.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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