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CLC number: R384.4; R757.3

On-line Access: 2012-06-29

Received: 2012-06-01

Revision Accepted: 2012-08-29

Crosschecked: 2012-09-04

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Journal of Zhejiang University SCIENCE B 2012 Vol.13 No.10 P.763-768


Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites

Author(s):  Ya-e Zhao, Zheng-hang Wang, Yang Xu, Ji-ru Xu, Wen-yan Liu, Meng Wei, Chu-ying Wang

Affiliation(s):  Department of Immunology and Pathogen Biology, Xian Jiaotong University College of Medicine, Xian 710061, China

Corresponding email(s):   zhaoyae@mail.xjtu.edu.cn, xujiru@mail.xjtu.edu.cn

Key Words:  Demodex, Chitin synthase genes, Cloning and sequencing, Phylogenetic study, Function analysis

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Ya-e Zhao, Zheng-hang Wang, Yang Xu, Ji-ru Xu, Wen-yan Liu, Meng Wei, Chu-ying Wang. Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites[J]. Journal of Zhejiang University Science B, 2012, 13(10): 763-768.

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journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

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%T Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites
%A Ya-e Zhao
%A Zheng-hang Wang
%A Yang Xu
%A Ji-ru Xu
%A Wen-yan Liu
%A Meng Wei
%A Chu-ying Wang
%J Journal of Zhejiang University SCIENCE B
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%D 2012
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200155

T1 - Cloning and sequence analysis of chitin synthase gene fragments of Demodex mites
A1 - Ya-e Zhao
A1 - Zheng-hang Wang
A1 - Yang Xu
A1 - Ji-ru Xu
A1 - Wen-yan Liu
A1 - Meng Wei
A1 - Chu-ying Wang
J0 - Journal of Zhejiang University Science B
VL - 13
IS - 10
SP - 763
EP - 768
%@ 1673-1581
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PB - Zhejiang University Press & Springer
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DOI - 10.1631/jzus.B1200155

To our knowledge, few reports on Demodex studied at the molecular level are available at present. In this study our group, for the first time, cloned, sequenced and analyzed the chitin synthase (CHS) gene fragments of Demodex folliculorum, Demodex brevis, and Demodex canis (three isolates from each species) from Xi’an China, by designing specific primers based on the only partial sequence of the CHS gene of D. canis from Japan, retrieved from GenBank. Results show that amplification was successful only in three D. canis isolates and one D. brevis isolate out of the nine Demodex isolates. The obtained fragments were sequenced to be 339 bp for D. canis and 338 bp for D. brevis. The CHS gene sequence similarities between the three Xi’an D. canis isolates and one Japanese D. canis isolate ranged from 99.7% to 100.0%, and those between four D. canis isolates and one D. brevis isolate were 99.1%–99.4%. Phylogenetic trees based on maximum parsimony (MP) and maximum likelihood (ML) methods shared the same clusters, according with the traditional classification. Two open reading frames (ORFs) were identified in each CHS gene sequenced, and their corresponding amino acid sequences were located at the catalytic domain. The relatively conserved sequences could be deduced to be a CHS class A gene, which is associated with chitin synthesis in the integument of Demodex mites.

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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