CLC number: Q943.2
On-line Access: 2017-08-08
Received: 2016-05-17
Revision Accepted: 2016-09-10
Crosschecked: 2017-07-25
Cited: 0
Clicked: 4984
Lu-yao Wang, Ying Zhang, Xue-qing Fu, Ting-ting Zhang, Jia-wei Ma, Li-da Zhang, Hong-mei Qian, Ke-xuan Tang, Shan Li, Jing-ya Zhao. Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua[J]. Journal of Zhejiang University Science B, 2017, 18(8): 662-673.
@article{title="Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua",
author="Lu-yao Wang, Ying Zhang, Xue-qing Fu, Ting-ting Zhang, Jia-wei Ma, Li-da Zhang, Hong-mei Qian, Ke-xuan Tang, Shan Li, Jing-ya Zhao",
journal="Journal of Zhejiang University Science B",
volume="18",
number="8",
pages="662-673",
year="2017",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1600223"
}
%0 Journal Article
%T Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua
%A Lu-yao Wang
%A Ying Zhang
%A Xue-qing Fu
%A Ting-ting Zhang
%A Jia-wei Ma
%A Li-da Zhang
%A Hong-mei Qian
%A Ke-xuan Tang
%A Shan Li
%A Jing-ya Zhao
%J Journal of Zhejiang University SCIENCE B
%V 18
%N 8
%P 662-673
%@ 1673-1581
%D 2017
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600223
TY - JOUR
T1 - Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AaICS1) gene from Artemisia annua
A1 - Lu-yao Wang
A1 - Ying Zhang
A1 - Xue-qing Fu
A1 - Ting-ting Zhang
A1 - Jia-wei Ma
A1 - Li-da Zhang
A1 - Hong-mei Qian
A1 - Ke-xuan Tang
A1 - Shan Li
A1 - Jing-ya Zhao
J0 - Journal of Zhejiang University Science B
VL - 18
IS - 8
SP - 662
EP - 673
%@ 1673-1581
Y1 - 2017
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600223
Abstract: isochorismate synthase (ICS) is a crucial enzyme in the salicylic acid (SA) synthesis pathway. The full-length complementary DNA (cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene, named AaICS1, contained a 1710-bp open reading frame, which encoded a protein with 570 amino acids. Bioinformatics and comparative study revealed that the polypeptide protein of AaICS1 had high homology with ICSs from other plant species. Southern blot analysis suggested that AaICS1 might be a single-copy gene. Analysis of the 1470-bp promoter of AaICS1 identified distinct cis-acting regulatory elements, including TC-rich repeats, MYB binding site (MBS), and TCA-elements. An analysis of AaICS1 transcript levels in multifarious tissues of A. annua using quantitative real-time polymerase chain reaction (qRT-PCR) showed that old leaves had the highest transcription levels. AaICS1 was up-regulated under wounding, drought, salinity, and SA treatments. This was corroborated by the presence of the predicted cis-acting elements in the promoter region of AaICS1. Overexpressing transgenic plants and RNA interference transgenic lines of AaICS1 were generated and their expression was compared. High-performance liquid chromatography (HPLC) results from leaf tissue of transgenic A. annua showed an increase in artemisinin content in the overexpressing plants. These results confirm that AaICS1 is involved in the isochorismate pathway.
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