Full Text:   <1034>

Summary:  <452>

CLC number: S828

On-line Access: 2015-06-08

Received: 2014-09-26

Revision Accepted: 2015-03-12

Crosschecked: 2015-05-13

Cited: 4

Clicked: 2070

Citations:  Bibtex RefMan EndNote GB/T7714


Ze-meng Feng


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Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.524-532


Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet

Author(s):  Sheng-ping Wang, Yun-ling Gao, Gang Liu, Dun Deng, Rong-jun Chen, Yu-zhe Zhang, Li-li Li, Qing-qi Wen, Yong-qing Hou, Ze-meng Feng, Zhao-hui Guo

Affiliation(s):  Research Center of Healthy Breeding Livestock & Poultry, Hunan Engineering & Research Center of Animal & Poultry Science, Key Lab Agro-ecology Processing Subtropical Region, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China; more

Corresponding email(s):   zemengfeng2006@163.com, funfarm@126.com

Key Words:  Adropin, Energy homeostasis-associated (Enho) gene, Gene coloning, Piglets, RT-PCR

Sheng-ping Wang, Yun-ling Gao, Gang Liu, Dun Deng, Rong-jun Chen, Yu-zhe Zhang, Li-li Li, Qing-qi Wen, Yong-qing Hou, Ze-meng Feng, Zhao-hui Guo. Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet[J]. Journal of Zhejiang University Science B, 2015, 16(6): 524-532.

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author="Sheng-ping Wang, Yun-ling Gao, Gang Liu, Dun Deng, Rong-jun Chen, Yu-zhe Zhang, Li-li Li, Qing-qi Wen, Yong-qing Hou, Ze-meng Feng, Zhao-hui Guo",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet
%A Sheng-ping Wang
%A Yun-ling Gao
%A Gang Liu
%A Dun Deng
%A Rong-jun Chen
%A Yu-zhe Zhang
%A Li-li Li
%A Qing-qi Wen
%A Yong-qing Hou
%A Ze-meng Feng
%A Zhao-hui Guo
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 524-532
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400260

T1 - Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet
A1 - Sheng-ping Wang
A1 - Yun-ling Gao
A1 - Gang Liu
A1 - Dun Deng
A1 - Rong-jun Chen
A1 - Yu-zhe Zhang
A1 - Li-li Li
A1 - Qing-qi Wen
A1 - Yong-qing Hou
A1 - Ze-meng Feng
A1 - Zhao-hui Guo
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 524
EP - 532
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400260

The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.




Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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