Full Text:   <1123>

Summary:  <455>

CLC number: S836

On-line Access: 2015-06-08

Received: 2014-11-30

Revision Accepted: 2015-03-18

Crosschecked: 2015-05-13

Cited: 10

Clicked: 1982

Citations:  Bibtex RefMan EndNote GB/T7714

 ORCID:

Xiu-qi Wang

http://orcid.org/0000-0003-2033-9485

-   Go to

Article info.
Open peer comments

Journal of Zhejiang University SCIENCE B 2015 Vol.16 No.6 P.511-523

http://doi.org/10.1631/jzus.B1400340


Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)


Author(s):  Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan

Affiliation(s):  College of Animal Science, South China Agricultural University / Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture / Guangdong Provincial Key Laboratory of Agro-Animal Genomics / South China Collaborative Innovatio Center for Poultry Disease Control and Product Safety, Guangzhou 510642, China; more

Corresponding email(s):   xqwang@scau.edu.cn, yanhc@scau.edu.cn

Key Words:  Embryonic growth, Nutrient transporters, Gene expression, Small intestine, Pigeons


Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan. Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)[J]. Journal of Zhejiang University Science B, 2015, 16(6): 511-523.

@article{title="Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)",
author="Ming-xia Chen, Xiang-guang Li, Jun-xian Yang, Chun-qi Gao, Bin Wang, Xiu-qi Wang, Hui-chao Yan",
journal="Journal of Zhejiang University Science B",
volume="16",
number="6",
pages="511-523",
year="2015",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1400340"
}

%0 Journal Article
%T Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)
%A Ming-xia Chen
%A Xiang-guang Li
%A Jun-xian Yang
%A Chun-qi Gao
%A Bin Wang
%A Xiu-qi Wang
%A Hui-chao Yan
%J Journal of Zhejiang University SCIENCE B
%V 16
%N 6
%P 511-523
%@ 1673-1581
%D 2015
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1400340

TY - JOUR
T1 - Growth of embryo and gene expression of nutrient transporters in the small intestine of the domestic pigeon (Columba livia)
A1 - Ming-xia Chen
A1 - Xiang-guang Li
A1 - Jun-xian Yang
A1 - Chun-qi Gao
A1 - Bin Wang
A1 - Xiu-qi Wang
A1 - Hui-chao Yan
J0 - Journal of Zhejiang University Science B
VL - 16
IS - 6
SP - 511
EP - 523
%@ 1673-1581
Y1 - 2015
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1400340


Abstract: 
The objective of this study was to investigate the relationship between gene expression of nutrient (amino acid, peptide, sodium and proton) transporters in the small intestine and embryonic growth in domestic pigeons (Columba livia). One hundred and twenty-five fertilized eggs were randomly assigned into five groups and were incubated under optimal conditions (temperature of 38.1 °C and relative humidity of 55%). Twenty embryos/birds from each group were sacrificed by cervical dislocation on embryonic day (E) 9, 11, 13, 15 and day of hatch (DOH). The eggs, embryos (without yolk sac), and organs (head, brain, heart, liver, lungs, kidney, gizzard, small intestine, legs, and thorax) were dissected, cleaned, and weighed. small intestine samples were collected for RNA isolation. The mRNA abundance of intestinal nutrient transporters was evaluated by real-time reverse transcription-polymerase chain reaction (RT-PCR). We classified these ten organs into four types according to the changes in relative weight during embryonic development. In addition, the gene expression of nutrient transporters was differentially regulated by embryonic day. The mRNA abundances of b0,+AT, EAAT3, y+LAT2, PepT1, LAT4, NHE2, and NHE3 increased linearly with age, whereas mRNA abundances of CAT1, CAT2, LAT1, EAAT2, SNAT1, and SNAT2 were increased to higher levels on E9 or E11 and then decreased to lower levels until DOH. The results of correlation analysis showed that the gene expressions of b0,+AT, EAAT3, PepT1, LAT4, NHE2, NHE3, and y+LAT2 had positive correlations with body weight (0.71<correlation coefficient (CC)<0.82, P<0.0001), while CAT1, CAT2, EAAT2, SNAT1, and SNAT2 had negative correlations with body weight (−0.86<CC<−0.64, P<0.0001). The gene expressions of b0,+AT, EAAT3, LAT4, PepT1, NHE2, NHE3, and y+LAT2 showed positive correlations with intestinal weight (0.80<CC<0.91, P<0.0001), while CAT1, CAT2, and EAAT2 showed negative correlations with intestinal weight (−0.84<CC<−0.67, P<0.0001). It was concluded that the differences between growth trajectories of organs and gene expression of nutrient transporters in small intestine were due to their functional and physiological properties, which provided a comprehensive study of amino acid and peptide transporter mRNA in the small intestine during embryonic growth of pigeons.

家鸽胚胎的生长与其小肠营养素转运载体基因表达

目的:探索家鸽胚胎期器官生长发育及其小肠营养素转运载体基因表达规律,为家鸽胚胎期的营养调控提供理论基础。
创新点:第一次较全面地研究了家鸽胚胎期小肠氨基酸转运载体基因表达规律。
方法:利用实时荧光定量逆转录聚合酶链反应(real-timeRT-PCR)技术,同时结合了多内标校正方法对小肠营养素转运载体基因表达模式进行分析。
结论:在家鸽胚胎期发育的过程中,不同的器官生长模式不完全一致。营养素转运载体b0,+ATEAAT3LAT4PepT1NHE2NHE3y+LAT2基因的表达与小肠重量呈正相关关系,CAT1CAT2EAAT2基因的表达与小肠重量呈负相关关系。

关键词:胚胎生长;营养素转运载体;基因表达;小肠;家鸽

Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article

Reference

[1]Al-Murrani, W.K., 1978. Maternal effects on embryonic and postembryonic growth in poultry. Br. Poult. Sci., 19(3):277-281.

[2]Azzam, M.A., Mortola, J.P., 2007. Organ growth in chicken embryos during hypoxia: implications on organ “sparing” and “catch-up growth”. Respir. Physiol. Neurobiol., 159(2):155-162.

[3]Bröer, S., 2008. Amino acid transport across mammalian intestinal and renal epithelia. Physiol. Rev., 88(1):249-286.

[4]Chaudhry, F.A., Reimer, R.J., Edwards, R.H., 2002. The glutamine commute: take the N line and transfer to the A. J. Cell Biol., 157(3):349-355.

[5]Closs, E.I., Boissel, J.P., Habermeier, A., et al., 2006. Structure and function of cationic amino acid transporters (CATs). J. Membr. Biol., 213(2):67-77.

[6]Daniel, H., 2004. Molecular and integrative physiology of intestinal peptide transport. Annu. Rev. Physiol., 66:361-384.

[7]de Oliveira, J.E., Druyan, S., Uni, Z., et al., 2009. Prehatch intestinal maturation of turkey embryos demonstrated through gene expression patterns. Poult. Sci., 88(12):2600-2609.

[8]Dong, X.Y., Wang, Y.M., Dai, L., et al., 2012a. Posthatch development of intestinal morphology and digestive enzyme activities in domestic pigeons (Columba livia). Poult. Sci., 91(8):1886-1892.

[9]Dong, X.Y., Wang, Y.M., Yuan, C., et al., 2012b. The ontogeny of nutrient transporter and digestive enzyme gene expression in domestic pigeon (Columba livia) intestine and yolk sac membrane during pre- and posthatch development. Poult. Sci., 91(8):1974-1982.

[10]Finkler, M.S., van Orman, J.B., Sotherland, P.R., 1998. Experimental manipulation of egg quality in chickens: influence of albumen and yolk on the size and body composition of near-term embryos in a precocial bird. J. Comp. Physiol. B, 168(1):17-24.

[11]Filippi, L., Hironaka, M., Nomakuchi, S., 2012. Individual variation in trophic egg production: evidence for maternal manipulation in response to resource and competition levels. Ethology, 118(5):503-510.

[12]Gilbert, E.R., Li, H., Ernmersonj, D.A., et al., 2007. Developmental regulation of nutrient transporter and enzyme mRNA abundance in the small intestine of broilers. Poult. Sci., 86(8):1739-1753.

[13]Humphrey, B.D., Stephensen, C.B., Calvert, C.C., et al., 2006. Lysine deficiency and feed restriction independently alter cationic amino acid transporter expression in chickens (Gallus gallus domesticus). Comp. Biochem. Physiol. A Mol. Integr. Physiol., 143(2):218-227.

[14]Hirst, B.H., 1993. Dietary regulation of intestinal nutrient carriers. Proc. Nutr. Soc., 52(2):315-324.

[15]Hyde, R., Taylor, P.M., Hundal, H.S., 2003. Amino acid transporters: roles in amino acid sensing and signalling in animal cells. Biochem. J., 373(Pt 1):1-18.

[16]Ipek, A., Sahan, U., Baycan, S.C., et al., 2014. The effects of different eggshell temperatures on embryonic development, hatchability, chick quality, and first-week broiler. Poult. Sci., 93(2):464-472.

[17]Kanai, Y., Segawa, H., Miyamoto, K.I., et al., 1998. Expression cloning and characterization of a transporter for large neutral amino acids activated by the heavy chain of 4F2 antigen (CD98). J. Biol. Chem., 273(37):23629-23632.

[18]Leibach, F.H., Ganapathy, V., 1996. Peptide transporters in the intestine and the kidney. Annu. Rev. Nutr., 16:99-119.

[19]Li, X.G., Yan, H.C., Zeng, P.L., et al., 2011. Comparison of difference and ontogenetic expression of EAATs mRNA in the small intestine of broiler chick embryo. Sci. Agric. Sin., 44(21):4474-4480 (in Chinese).

[20]Li, X.G., Yan, H.C., Zeng, P.L., et al., 2012. The ontogenetic expression of PepT1 and NHEs mRNA in the small intestine of broiler embryos. Acta Agric. Boreali-Sin., 27(5):96-100 (in Chinese).

[21]Li, X.G., Chen, X.L., Wang, X.Q., 2013. Changes in relative organ weights and intestinal transporter gene expression in embryos from White Plymouth Rock and WENS Yellow Feather Chickens. Comp. Biochem. Physiol. A Mol. Integr. Physiol., 164(2):368-375.

[22]Li, X.G., Sui, W.G., Yan, H.C., et al., 2014. The in ovo administration of L-trans pyrrolidine-2,4-dicarboxylic acid regulates small intestinal growth in chicks. Animal, 8(10):1677-1683.

[23]Mastroberardino, L., Spindler, B., Pfeiffer, R., et al., 1998. Amino-acid transport by heterodimers of 4F2hc/CD98 and members of a permease family. Nature, 39(6699):288-291.

[24]McGivan, J.D., Bungard, C.I., 2007. The transport of glutamine into mammalian cells. Front. Biosci., 12:874-882.

[25]Miska, K.B., Fetterer, R.H., Wong, E.A., 2014. The mRNA expression of amino acid transporters, aminopeptidase N, and the di- and tri-peptide transporter PepT1 in the embryo of the domesticated chicken (Gallus gallus) shows developmental regulation. Poult. Sci., 93(9):2262-2270.

[26]Moran, E.T., 2007. Nutrition of the developing embryo and hatchling. Poult. Sci., 86(5):1043-1049.

[27]Mott, C.R., Siegel, P.B., Webb, K.E., et al., 2008. Gene expression of nutrient transporters in the small intestine of chickens from lines divergently selected for high or low juvenile body weight. Poult. Sci., 87(11):2215-2224.

[28]Noy, Y., Sklan, D., 1998. Yolk utilisation in the newly hatched poult. Br. Poult. Sci., 39(3):446-451.

[29]Noy, Y., Uni, Z., Sklan, D., 1996. Routes of yolk utilisation in the newly-hatched chick. Br. Poult. Sci., 37(5):987-996.

[30]Orlowski, J., Grinstein, S., 1997. Na+/H+ exchangers of mammalian cells. J. Biol. Chem., 272(36):22373-22376.

[31]Poncet, N., Taylor, P.M., 2013. The role of amino acid transporters in nutrition. Curr. Opin. Clin. Nutr. Metab. Care, 16(1):57-65.

[32]Speier, J.S., Yadgary, L., Uni, Z., et al., 2012. Gene expression of nutrient transporters and digestive enzymes in the yolk sac membrane and small intestine of the developing embryonic chick. Poult. Sci., 91(8):1941-1949.

[33]Stepińska, M., Mroz, E., Jankowski, J., 2012. The effect of dietary selenium source on embryonic development in turkeys. Folia Biol. (Krakow), 60(3-4):235-241.

[34]Sun, X., Zhang, H., Sheikhahmadi, A., et al., 2015. Effects of heat stress on the gene expression of nutrient transporters in the jejunum of broiler chickens (Gallus gallus domesticus). Int. J. Biometeorol., 59(2):127-135.

[35]Tanaka, K., Yamamoto, A., Fujita, T., 2005. Functional expression and adaptive regulation of Na+-dependent neutral amino acid transporter SNAT2/ATA2 in normal human astrocytes under amino acid starved condition. Neurosci. Lett., 378(2):70-75.

[36]Tangara, M., Chen, W., Xu, J., et al., 2010. Effects of in ovo feeding of carbohydrates and arginine on hatchability, body weight, energy metabolism and perinatal growth in duck embryos and neonates. Br. Poult. Sci., 51(5):602-608.

[37]Tazawa, H., Visschedijk, A.H., Wittmann, J., et al., 1983. Gas exchange, blood gases and acid-base status in the chick before, during and after hatching. Resp. Physiol., 53(2):173-185.

[38]Tona, K., Onagbesan, O., de Ketelaere, B., et al., 2004. Effects of age of broiler breeders and egg storage on egg quality, hatchability, chick quality, chick weight, and chick posthatch growth to forty-two days. J. Appl. Poult. Res., 13(1):10-18.

[39]Vandesompele, J., de Preter, K., Pattyn, F., et al., 2002. Accurate normalization of real-time quantitative rt-pcr data by geometric averaging of multiple internal control genes. Genome Biol., 3(7):12.

[40]Xie, P., Zhang, A.T., Wang, C., et al., 2012. Molecular cloning, characterization, and expression analysis of fatty acid translocase (FAT/CD36) in the pigeon (Columba livia domestica). Poult. Sci., 91(7):1670-1679.

[41]Xie, P., Liu, L.L., Wang, C., et al., 2013. Molecular cloning, characterization, and mRNA expression of intestinal fatty acid binding protein (I-FABP) in Columba livia. J. Poult. Sci., 50(1):9-19.

[42]Zeng, P.L., Li, X.G., Wang, X.Q., et al., 2011. The relationship between gene expression of cationic and neutral amino acid transporters in the small intestine of chick embryos and chick breed, development, sex, and egg amino acid concentration. Poult. Sci., 90(11):2548-2556.

Open peer comments: Debate/Discuss/Question/Opinion

<1>

Please provide your name, email address and a comment





Journal of Zhejiang University-SCIENCE, 38 Zheda Road, Hangzhou 310027, China
Tel: +86-571-87952783; E-mail: cjzhang@zju.edu.cn
Copyright © 2000 - Journal of Zhejiang University-SCIENCE