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Received: 2012-10-30

Revision Accepted: 2013-01-16

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.7 P.604-614


Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD

Author(s):  Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng

Affiliation(s):  Institute of Pharmaceutical Informatics, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wangsf@zju.edu.cn

Key Words:  Traditional Chinese medicine (TCM) compound recipe, Xiongdankaiming tablet, HPLC-UV/ELSD, HPLC-MS, Quality control The online version of this article contains supplementary materialssupplementary materials

Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng. Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD[J]. Journal of Zhejiang University Science B, 2013, 14(7): 604-614.

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author="Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD
%A Shu-fang Wang
%A Jing Leng
%A Yi-min Xu
%A Mei-ling Feng
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 7
%P 604-614
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200295

T1 - Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD
A1 - Shu-fang Wang
A1 - Jing Leng
A1 - Yi-min Xu
A1 - Mei-ling Feng
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 7
SP - 604
EP - 614
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200295

xiongdankaiming tablet (XDKMT), a well-known compound in traditional Chinese medicine, is widely used for the treatment of acute iridocyclitis and primary open-angle glaucoma. In this paper, accurate and reliable methods were developed for the identification of 20 constituents using high-performance liquid chromatography with photo-diode array and electron spray ionization-mass spectrometry (HPLC-PDA/ESI-MSn), and determination of nine of the constituents (chlorogenic acid, gentiopicroside, isochlorogenic acid B, diosmetin-7-O-β-d-glucopyranoside, apigenin, diosmetin, tauroursodeoxycholic acid, acacetin, and taurochenodeoxycholic acid) was developed using HPLC with ultraviolet absorption detector and evaporative light scattering detector (HPLC-UV/ELSD) for the first time. The best results were obtained on a Zorbax SB-C18 column with gradient elution using water (0.1% formic acid) (A) and methanol (0.1% formic acid) (B) at a flow rate of 0.7 ml/min. Tauroursodeoxycholic acid and taurochenodeoxycholic acid, owing to their low UV absorption, were detected by ELSD. The other seven compounds were analyzed by HPLC-UV with variable wavelengths. The calibration curves of all nine constituents showed good linear regression (R2>0.9996) within the linearity ranges. The limits of detection and quantification were in the ranges of 0.0460–9.90 μg/ml and 0.115–24.8 μg/ml, respectively. The accuracy, in terms of recovery, varied from 95.3% to 104.9% with relative standard deviations (RSDs) less than 4.4%. Precision (with the intra- and inter-day variations less than 4.4%) was also suitable for its intended use. The developed method was successfully applied for the analysis of major components in XDKMT, which provides an appropriate method for the quality control of XDKMT.

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[1]Cao, X.Y., Wang, Z.Z., 2010. Simultaneous determination of four iridoid and secoiridoid glycosides and comparative analysis of Radix gentianae Macrophyllae and their related substitutes by HPLC. Phytochem. Anal., 21(4):348-354.

[2]Cheng, W.M., Li, J., You, T.P., Hu, C.M., 2005. Anti-inflammatory and immunomodulatory activities of the extracts from the inflorescence of Chrysanthemum indicum Linné. J. Ethnopharmacol., 101(1-3):334-337.

[3]Cui, H., Liu, J.J., Fu, S.Y., Liu, H.L., Hou, Y.S., Jin, D., 2006. Experimental study of the permeability of Fel Ursi’s in eye. Chin. J. Ophthalmol., 42(11):1023-1025 (in Chinese).

[4]Gao, Y.M., Song, Q., Chen, H.L., Wei, S.H., Yang, Y.Q., Zhang, L.Y., Wang, J.K., 2007. HPCE determination of gentiopicroside in Radix gentianae. Chin. J. Pharm. Anal., 27(10):1572-1574 (in Chinese).

[5]Gong, R.Y., Zhang, D., Lv, Z.L., Du, L.P., Zhang, L.D., Li, J.R., Qiao, X.L., 2007. Extration of sodium taurochenodeoxycholate and comparison of its pharmacological effect with sodium chenodeoxycholate. Fine Chem., 24(10):975-978 (in Chinese).

[6]Hong, L., Li, Y.J., Qu, B., 2008. Observation of the influence of Xiongdankaiming tablet on relieving the visual fatigue symptoms of young people. Chin. J. Modern Drug Appl., 5(2):62-63 (in Chinese).

[7]Kim, K.J., Kim, Y.H., Yu, H.H., Jeong, S.I., Cha, J.D., Kil, B.S., You, Y.O., 2003. Antibacterial activity and chemical composition of essential oil of Chrysanthemum boreale. Planta Med., 69(3):274-277.

[8]Kondo, Y., Takano, F., Hojo, H., 1994. Suppression of chemically and immunologically induced hepatic injuries by gentiopicroside in mice. Planta Med., 60(5):414-416.

[9]Kumarasamy, Y., Nahar, L., Sarker, S.D., 2003. Bioactivity of gentiopicroside from the aerial parts of Centaurium erythraea. Fitoterapia, 74(1-2):151-154.

[10]Li, H., Wang, S.W., Zhang, B.L., Xie, Y.H., Yang, Q., Cao, W., Wang, J.B., 2011. Simultaneous quantitative determination of 9 active components in traditional Chinese medicinal preparation ShuangDan oral liquid by RP-HPLC coupled with photodiode array detection. J. Pharm. Biomed. Anal., 56(4):820-824.

[11]Li, S.P., Zhao, J., Yang, B., 2011. Strategies for quality control of Chinese medicines. J. Pharm. Biomed. Anal., 55(4):802-809.

[12]Liu, B., Li, X., Wang, H., 2005. Clinical observation of Xiongdankaiming tablet on prevention and treatment of the postoperative complications caused by phacoemulsification intraocular lens implantation. Lishizhen Med. Mat. Med. Res., 4(16):341 (in Chinese).

[13]Miyazawa, M., Hisama, M., 2003. Antimutagenic activity of flavonoids from Chrysanthemum morifolium. Biosci. Biotech. Biochem., 67(10):2091-2099.

[14]Qin, S., Wen, X.S., 2011. Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC. J. Chin. Mat. Med., 36(11):1474-1477 (in Chinese).

[15]Wang, J.Y., Zhang, T.T., Xu, Q.X., Sun, H., Zhou, Q., Wu, Y.M., 2011. Study on betaine by electrospray ionization mass spectrometry. Sugar Crops China, 3(3):23-25 (in Chinese).

[16]Wang, L.M., 2010. Determination of ridoids in Radix gentianae from various habitats. Chin. Tradit. Patent Med., 32(11):1941-1945 (in Chinese).

[17]Wang, S.F., Xu, Y.M., Jiang, W., Zhang, Y.F., 2013. Isolation and identification of constituents with anti-inflammatory activities from Gentiana triflora Pall. Planta Med., 79(8):680-686.

[18]Xie, P.S., Liang, G.H., Mao, W.Z., 1981. Analysis and quality investigation of bear bile. Chin. J. Pharm. Anal., 1(3):137 (in Chinese).

[19]Yin, J., Zhang, Z.W., Yu, W.J., Liao, J.Y., Luo, X.G., Shen, Y.J., 2010. Stachydrine, a major constituent of the Chinese herb Leonurus heterophyllus sweet, ameliorates human umbilical vein endothelial cells injury induced by anoxia-reoxygenation. Am. J. Chin. Med., 38(1):157-171.

[20]Zhang, S., Dong, S.Q., Chi, L.Z., He, P.G., Wang, Q.J., Fang, Y.Z., 2008. Simultaneous determination of flavonoids in Chrysanthemum by capillary zone electrophoresis with running buffer modifiers. Talanta, 76(4):780-784.

[21]Zhao, M., Qi, Y., 2007. Experimental studies of Xiongdankaiming tablet on lowering the intraocular pressure. J. Changchun Univ. Tradit. Chin. Med., 1(23):27-28 (in Chinese).

[22]Zhao, M., Qi, Y., Han, Y., 1999. Animal experiments on analgesic and anti-inflammatory effects of Xiongdankaiming tablet. J. Norman Bethune Univ. Med. Sci., 3(25):44 (in Chinese).

[23]Zhao, Y., Zan, L.X., Sun, W.J., 2006. HPLC-ELSD determination of tauroursodeoxycholic acid and taurochenodeoxycholic acid in bear biliary drainage powder. Chin. J. Pharm. Anal., 26(1):127-129 (in Chinese).

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