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Received: 2012-10-30

Revision Accepted: 2013-01-16

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Journal of Zhejiang University SCIENCE B 2013 Vol.14 No.7 P.604-614


Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD

Author(s):  Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng

Affiliation(s):  Institute of Pharmaceutical Informatics, Zhejiang University, Hangzhou 310058, China; more

Corresponding email(s):   wangsf@zju.edu.cn

Key Words:  Traditional Chinese medicine (TCM) compound recipe, Xiongdankaiming tablet, HPLC-UV/ELSD, HPLC-MS, Quality control The online version of this article contains supplementary materialssupplementary materials

Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng. Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD[J]. Journal of Zhejiang University Science B, 2013, 14(7): 604-614.

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author="Shu-fang Wang, Jing Leng, Yi-min Xu, Mei-ling Feng",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD
%A Shu-fang Wang
%A Jing Leng
%A Yi-min Xu
%A Mei-ling Feng
%J Journal of Zhejiang University SCIENCE B
%V 14
%N 7
%P 604-614
%@ 1673-1581
%D 2013
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1200295

T1 - Identification and determination of major constituents in a traditional Chinese medicine compound recipe Xiongdankaiming tablet using HPLC-PDA/ESI-MSn and HPLC-UV/ELSD
A1 - Shu-fang Wang
A1 - Jing Leng
A1 - Yi-min Xu
A1 - Mei-ling Feng
J0 - Journal of Zhejiang University Science B
VL - 14
IS - 7
SP - 604
EP - 614
%@ 1673-1581
Y1 - 2013
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1200295

xiongdankaiming tablet (XDKMT), a well-known compound in traditional Chinese medicine, is widely used for the treatment of acute iridocyclitis and primary open-angle glaucoma. In this paper, accurate and reliable methods were developed for the identification of 20 constituents using high-performance liquid chromatography with photo-diode array and electron spray ionization-mass spectrometry (HPLC-PDA/ESI-MSn), and determination of nine of the constituents (chlorogenic acid, gentiopicroside, isochlorogenic acid B, diosmetin-7-O-?-d-glucopyranoside, apigenin, diosmetin, tauroursodeoxycholic acid, acacetin, and taurochenodeoxycholic acid) was developed using HPLC with ultraviolet absorption detector and evaporative light scattering detector (HPLC-UV/ELSD) for the first time. The best results were obtained on a Zorbax SB-C18 column with gradient elution using water (0.1% formic acid) (A) and methanol (0.1% formic acid) (B) at a ?ow rate of 0.7 ml/min. Tauroursodeoxycholic acid and taurochenodeoxycholic acid, owing to their low UV absorption, were detected by ELSD. The other seven compounds were analyzed by HPLC-UV with variable wavelengths. The calibration curves of all nine constituents showed good linear regression (R2>0.9996) within the linearity ranges. The limits of detection and quantification were in the ranges of 0.04609.90 ?g/ml and 0.11524.8 ?g/ml, respectively. The accuracy, in terms of recovery, varied from 95.3% to 104.9% with relative standard deviations (RSDs) less than 4.4%. Precision (with the intra- and inter-day variations less than 4.4%) was also suitable for its intended use. The developed method was successfully applied for the analysis of major components in XDKMT, which provides an appropriate method for the quality control of XDKMT.

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