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On-line Access: 2016-12-05

Received: 2016-03-14

Revision Accepted: 2016-07-12

Crosschecked: 2016-11-17

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Young Soo Kim


Yongho Jeon


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Journal of Zhejiang University SCIENCE B 2016 Vol.17 No.12 P.931-940


Effects of rhizobacteria Paenibacillus polymyxa APEC136 and Bacillus subtilis APEC170 on biocontrol of postharvest pathogens of apple fruits

Author(s):  Young Soo Kim, Kotnala Balaraju, Yongho Jeon

Affiliation(s):  Department of Bioresource Sciences, Andong National University, Andong 36729, Korea; more

Corresponding email(s):   yongbac@andong.ac.kr

Key Words:  Anthracnose, Antagonistic activity, Biological control, White rot, Apple

Young Soo Kim, Kotnala Balaraju, Yongho Jeon. Effects of rhizobacteria Paenibacillus polymyxa APEC136 and Bacillus subtilis APEC170 on biocontrol of postharvest pathogens of apple fruits[J]. Journal of Zhejiang University Science B, 2016, 17(12): 931-940.

@article{title="Effects of rhizobacteria Paenibacillus polymyxa APEC136 and Bacillus subtilis APEC170 on biocontrol of postharvest pathogens of apple fruits",
author="Young Soo Kim, Kotnala Balaraju, Yongho Jeon",
journal="Journal of Zhejiang University Science B",
publisher="Zhejiang University Press & Springer",

%0 Journal Article
%T Effects of rhizobacteria Paenibacillus polymyxa APEC136 and Bacillus subtilis APEC170 on biocontrol of postharvest pathogens of apple fruits
%A Young Soo Kim
%A Kotnala Balaraju
%A Yongho Jeon
%J Journal of Zhejiang University SCIENCE B
%V 17
%N 12
%P 931-940
%@ 1673-1581
%D 2016
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1600117

T1 - Effects of rhizobacteria Paenibacillus polymyxa APEC136 and Bacillus subtilis APEC170 on biocontrol of postharvest pathogens of apple fruits
A1 - Young Soo Kim
A1 - Kotnala Balaraju
A1 - Yongho Jeon
J0 - Journal of Zhejiang University Science B
VL - 17
IS - 12
SP - 931
EP - 940
%@ 1673-1581
Y1 - 2016
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1600117

In this study, plant growth-promoting rhizobacteria (PGPR) were evaluated as potential biocontrol agents against postharvest pathogens of apple fruits. In vitro bioassays revealed that, out of 30 isolates screened, isolates APEC136 and APEC170 had the most significant inhibitory effects against the mycelial growth of several fungal pathogens. Analysis of 16S ribosomal RNA (rRNA) sequences identified the two effective isolates as Paenibacillus polymyxa and Bacillus subtilis, respectively. The two strains showed greater growth in brain-heart infusion broth than in other growth media. Treatment of harvested apples with suspensions of either strain reduced the symptoms of anthracnose disease caused by two fungal pathogens, Colletotrichum gloeosporioides and Colletotrichum acutatum, and white rot disease caused by Botryosphaeria dothidea. Increased productions of amylase and protease by APEC136, and increased productions of chitinase, amylase, and protease by APEC170 might have been responsible for inhibiting mycelial growth. The isolates caused a greater reduction in the growth of white rot than of anthracnose. These results indicate that the isolates APEC136 and APEC170 are promising agents for the biocontrol of anthracnose and white rot diseases in apples after harvest, and suggest that these isolates may be useful in controlling these diseases under field conditions.


方法:选择APEC136和APEC170两菌株为对象,通过16S rRNA序列分析进行菌种鉴定;研究不同的培养基、温度和pH值等条件对APEC136和APEC170生长影响;测定其对几丁质酶,淀粉酶,纤维素酶和蛋白酶的产量和活性的影响;分析其对胶孢炭疽菌(Colletotrichum gloeosporioides)、尖孢炭疽(Colletotrichum acutatum)和葡萄座腔菌(Botryosphaeria dothidea)的菌丝生长抑制作用,观察苹果采后炭疽病和白腐病症状的变化。
结论:16S rRNA序列分析结果发现两个拮抗菌株分别为多粘类芽孢杆菌和枯草芽孢杆菌。在脑心浸液肉汤(BHI)培养基中,这两个菌株生长最快。体外生物测定表明,在筛选的30株菌株中,APEC136和APEC170对胶孢炭疽菌、尖孢炭疽和葡萄座腔菌的菌丝生长的抑制作用最显著。APEC136和APEC170均减轻了苹果采后由病原真菌引起的炭疽病和白腐病症状。另外,APEC136增加了淀粉酶和蛋白酶产量,APEC170增加了几丁质酶、淀粉酶和蛋白酶产量,这些可能是其抑制菌丝生长的原因。综上所述,菌株APEC136和APEC170是有效的苹果采后炭疽病和白腐病的生物防治剂,可用于野外条件下的疾病控制。


Darkslateblue:Affiliate; Royal Blue:Author; Turquoise:Article


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