CLC number: Q24
On-line Access: 2024-08-27
Received: 2023-10-17
Revision Accepted: 2024-05-08
Crosschecked: 2010-09-03
Cited: 4
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Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin. Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts[J]. Journal of Zhejiang University Science B, 2010, 11(10): 801-805.
@article{title="Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts",
author="Yuriy G. Shckorbatov, Vladimir N. Pasiuga, Elena I. Goncharuk, Tatiana Ph. Petrenko, Valentin A. Grabina, Nicolay N. Kolchigin, Dmitry D. Ivanchenko, Victor N. Bykov, Oleksandr M. Dumin",
journal="Journal of Zhejiang University Science B",
volume="11",
number="10",
pages="801-805",
year="2010",
publisher="Zhejiang University Press & Springer",
doi="10.1631/jzus.B1000051"
}
%0 Journal Article
%T Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts
%A Yuriy G. Shckorbatov
%A Vladimir N. Pasiuga
%A Elena I. Goncharuk
%A Tatiana Ph. Petrenko
%A Valentin A. Grabina
%A Nicolay N. Kolchigin
%A Dmitry D. Ivanchenko
%A Victor N. Bykov
%A Oleksandr M. Dumin
%J Journal of Zhejiang University SCIENCE B
%V 11
%N 10
%P 801-805
%@ 1673-1581
%D 2010
%I Zhejiang University Press & Springer
%DOI 10.1631/jzus.B1000051
TY - JOUR
T1 - Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts
A1 - Yuriy G. Shckorbatov
A1 - Vladimir N. Pasiuga
A1 - Elena I. Goncharuk
A1 - Tatiana Ph. Petrenko
A1 - Valentin A. Grabina
A1 - Nicolay N. Kolchigin
A1 - Dmitry D. Ivanchenko
A1 - Victor N. Bykov
A1 - Oleksandr M. Dumin
J0 - Journal of Zhejiang University Science B
VL - 11
IS - 10
SP - 801
EP - 805
%@ 1673-1581
Y1 - 2010
PB - Zhejiang University Press & Springer
ER -
DOI - 10.1631/jzus.B1000051
Abstract: To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm2, exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm2 produced no effect, and irradiation at the intensities of 10 and 100 µW/cm2 induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one.
[1]Garaj-Vrhovac, V., 1999. Micronucleus assay and lymphocyte mitotic activity in risk assessment of occupational exposure to microwave radiation. Chemosphere, 39(13):2301-2312.
[2]Garaj-Vrhovac, V., Horvat, D., Koren, Z., 1990. The effect of microwave radiation on the cell genome. Mutat. Res. Lett., 243(2):87-93.
[3]Garaj-Vrhovac, V., Horvat, D., Koren, Z., 1991. The relationship between colony-forming ability, chromosome aberrations and incidence of micronuclei in V79 Chinese hamster cells exposed to microwave radiation. Mutat. Res. Lett., 263(3):143-149.
[4]Garaj-Vrhovac, V., Fucic, A., Horvat, D., 1992. The correlation between the frequency of micronuclei and specific chromosome aberrations in human lymphocytes exposed to microwaves. Mutat. Res. Lett., 281(3):181-186.
[5]Görlitz, B.D., Müller, M., Ebert, S., Hecker, H., Kuster, N., Dasenbrock, C., 2005. Effects of 1-week and 6-week exposure to GSM/DCS radiofrequency radiation on micronucleus formation in B6C3F1 mice. Radiat. Res., 164(4):431-439.
[6]Hodgson, E., 2004. A Textbook of Modern Toxicology, 3rd Ed. John Wiley & Sons, New Jersey, p.14-16.
[7]Kiselev, R.I., Zalyubovskaya, N.P., 1973. Influence of electromagnetic waves of millimeter range on cell and some structural elements of cell. Uspekhi Fizicheskih Nauk (Success in Physical Sciences), 110(3):464-466 (in Russian).
[8]Lewin, B., 2004. Genes VIII. Upper Saddle River. Pearson Education Inc., New Jersey, p.1002.
[9]Luijsterburg, M.S., Dinant, C., Lans, H., Stap, J., Wiernasz, E., Lagerwerf, S., Warmerdam, D.O., Lindh, M., Brink, M.C., Jurek, W.J.W., et al., 2009. Heterochromatin protein 1 is recruited to various types of DNA damage. J. Cell Biol., 185(4):577-586.
[10]Michaelson, S.M., 1980. Microwave biological effects: an overview. Proc. IEEE, 68(1):40-49.
[11]Sanderson, A., Stewart, J., 1961. Nuclear sexing with acetoorcein. BMJ, 2(5259):1065-1067.
[12]Shckorbatov, Y.G., 1999. He-Ne laser light induced changes in the state of chromatin in human cells. Naturwissenschaften, 86(9):452-453.
[13]Shckorbatov, Y.G., Shakhbazov, V.G., Bogoslavsky, A.M., Rudenko, A.O., 1995. On age-related changes of cell membrane permeability in human buccal epithelium cells. Mech. Ageing Dev., 83(2):87-90.
[14]Shckorbatov, Y.G., Shakhbazov, V.G., Grigoryeva, N.N., Grabina, V.A., 1998. Microwave irradiation influences on the state of human cell nuclei. Bioelectromagnetics, 19(7):414-419.
[15]Shckorbatov, Y.G., Pasiuga, V.N., Kolchigin, N.N., Grabina, V.A., Batrakov, D.O., Kalashnikov, V.V., Ivanchenko, D.D., Bykov, V.N., 2009. The influence of differently polarized microwave radiation on chromatin in human cells. Int. J. Radiat. Biol., 85(4):322-329.
[16]Szmigielski, S., Luszak, M., Wiranowska, M., 1975. Kariometric observations of WISH cell cultures irradiated with 36 GHz microwaves. Folia Histochem. Cytochem., 13(3-4):151-159.
[17]Tillemans, A., 2008. Kontroverse um Radarstrahlung. wissenschaft.de. Available from http://www.wissenschaft. de/wissenschaft/hintergrund/297264.html [Accessed on Nov. 15, 2008] (in German).
[18]Trosic, I., Busljeta, I., Kasuba, V., Rozgaj, R., 2002. Micronucleus induction after whole-body microwave irradiation of rats. Mutat. Res., 521(1-2):73-79.
[19]Yao, K., Wu, W., Wang, K., Ni, S., Ye, P., Ye, Y., Ye, J., Sun, L., 2008. Electromagnetic noise inhibits radiofrequency radiation-induced DNA damage and reactive oxygen species increase in human lens epithelial cells. Mol. Vis., 19:964-969.
[20]Zeni, O., Schiavoni, A.S., Sannino, A., Antolini, A., Forigo, D., Bersani, F., Scarfi, M.R., 2003. Lack of genotoxic effects (micronucleus induction) in human lymphocytes exposed in vitro to 900 MHz electromagnetic fields. Radiat. Res., 160(2):152-158.
[21]Zotti-Martelli, L., Peccatori, M., Maggini, V., Ballardin, M., Barale, R., 2005. Individual responsiveness to induction of micronuclei in human lymphocytes after exposure in vitro to 1800-MHz microwave radiation. Mutat. Res., 582(1-2):42-52.
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